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Imaging Immune Surv...
Imaging Immune Surveillance of Individual Natural Killer Cells Confined in Microwell Arrays
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- Guldevall, Karolin (author)
- KTH,Cellens fysik
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- Vanherberghen, Bruno (author)
- KTH,Cellens fysik
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- Frisk, Thomas (author)
- Karolinska Institutet
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- Hurtig, Johan (author)
- Department of Chemsitry, University of Washington, Seattle, USA
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- Christakou, Athanasia (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Manneberg, Otto (author)
- Department of Environmental Health, Harvard School of Public Health, Boston, USA
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- Lindström, Sara (author)
- KTH,Nanobioteknologi
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- Andersson-Svahn, Helene (author)
- KTH,Nanobioteknologi
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- Wiklund, Martin (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Önfelt, Björn (author)
- Karolinska Institutet,KTH,Cellens fysik
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(creator_code:org_t)
- 2010-11-12
- 2010
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 5:11, s. e15453-
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https://doi.org/10.1...
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Abstract
Subject headings
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- New markers are constantly emerging that identify smaller and smaller subpopulations of immune cells. However, there is a growing awareness that even within very small populations, there is a marked functional heterogeneity and that measurements at the population level only gives an average estimate of the behaviour of that pool of cells. New techniques to analyze single immune cells over time are needed to overcome this limitation. For that purpose, we have designed and evaluated microwell array systems made from two materials, polydimethylsiloxane (PDMS) and silicon, for high-resolution imaging of individual natural killer (NK) cell responses. Both materials were suitable for short-term studies (<4 hours) but only silicon wells allowed long-term studies (several days). Time-lapse imaging of NK cell cytotoxicity in these microwell arrays revealed that roughly 30% of the target cells died much more rapidly than the rest upon NK cell encounter. This unexpected heterogeneity may reflect either separate mechanisms of killing or different killing efficiency by individual NK cells. Furthermore, we show that high-resolution imaging of inhibitory synapse formation, defined by clustering of MHC class I at the interface between NK and target cells, is possible in these microwells. We conclude that live cell imaging of NK-target cell interactions in multi-well microstructures are possible. The technique enables novel types of assays and allow data collection at a level of resolution not previously obtained. Furthermore, due to the large number of wells that can be simultaneously imaged, new statistical information is obtained that will lead to a better understanding of the function and regulation of the immune system at the single cell level.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- t-cells
- single cells
- imunological synapse
- microfluidic device
- limph-node
- on-chip
- activation
- platform
- segregation
- cytometry
- Bioengineering
- Bioteknik
Publication and Content Type
- ref (subject category)
- art (subject category)
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Guldevall, Karol ...
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Vanherberghen, B ...
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Frisk, Thomas
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Hurtig, Johan
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Christakou, Atha ...
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Manneberg, Otto
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Lindström, Sara
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Andersson-Svahn, ...
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Wiklund, Martin
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Önfelt, Björn
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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PLOS ONE
- By the university
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Royal Institute of Technology
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Karolinska Institutet