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Sökning: WFRF:(Lindström Sara) > Konferensbidrag

  • Resultat 1-7 av 7
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  • Lindström, Sara, et al. (författare)
  • A microwell chip for parallel culture and analysis of stem cells
  • 2009
  • Ingår i: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 1309-1311
  • Konferensbidrag (refereegranskat)abstract
    • With recent findings on the role of reprogramming factors on stem cells, in vitro screening assays for studying (de)-differentiation is of great interest. We developed a miniaturized stem cell screening chip that is easily accessible and provides means of rapidly studying thousands of individual stem/progenitor cell samples, using low reagent volumes. Results presented here include weeklong culturing and differentiation assays of mouse embryonic stem cells, mouse adult neural stem cells, and human embryonic stem cells. The possibility to maintain the cells as stem/progenitor cells over time was shown, as was isolation and clonal analysis.
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  • Lindström, Sara, et al. (författare)
  • High throughput single cell clone analysis
  • 2006
  • Ingår i: Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference. - : Japan Academic Association Inc. ; , s. 410-412
  • Konferensbidrag (refereegranskat)abstract
    • A novel microplate has been developed for high throughput single cell/clone analysis. Rapid single cell seeding using a conventional FACS into micro wells allows several thousands of single cells to be cultivated, short-term (72 h) or long-term (10-14 days), and analyzed individually. The platform requires a remarkably low number of cells, a major advantage when screening limited amounts of patient cell samples. Analysis of single cell heterogeneity and colony formation related to drug sensitivity can be accomplished in a high throughput manner.
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  • Lindström, Sara, et al. (författare)
  • PCR amplification and genetic analysis in a microwell cell cultivation chip
  • 2008
  • Ingår i: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. - : Chemical and Biological Microsystems Society. ; , s. 576-578
  • Konferensbidrag (refereegranskat)abstract
    • We present a method for long-term single cell/clone cultivation followed by cell lysis, DNA amplification and detection of PCR product in a chip containing 672 individual microwells. By performing all steps on-chip in microwells, the proliferation and cell morphology of every single cell or clone can be linked to its genetic information. In this study two mammalian cell lines (mutated A431 vs. wild type U-2 OS) were used as a model system for mutation screening in the p53 gene. The presented method could improve the sensitivity in mutation frequency analysis of heterogeneous tumor samples.
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  • Persson, Erik, et al. (författare)
  • Some challenges in connection with the construction of a Massive Open Online Course on ethical and societal aspects of AI
  • 2021
  • Ingår i: Hållbart lärarskap : universitetsundervisning i förändring – Proceedings från Humanistiska och Teologiska fakulteternas pedagogiska inspirationskonferens 2020 - universitetsundervisning i förändring – Proceedings från Humanistiska och Teologiska fakulteternas pedagogiska inspirationskonferens 2020. - 9789172674530 - 9789172674547 ; , s. 99-110
  • Konferensbidrag (refereegranskat)abstract
    • The Swedish government is at present investing heavily in making Sweden competitive in AI (Artificial Intelligence). As a part of that effort, Lund University has been commissioned with providing a series of MOOCs (Massive Open Online Courses) on different topics regarding AI, including several topics dealing with Humans and AI. As a comprehensive university, Lund University has an advantage in being able to facilitate collaboration across academic fields to meet multidisciplinary knowledge needs in the wider society for the future of sustainable AI design, implementation, and use. The courses aim at a very wide audience but are particularly suited for those who did not major in AI related subjects at the university but who nonetheless have to deal with AI in their work. This resulted in a suite of courses of which one deals with societal challenges and ethical issues in AI. The course covers algorithmic bias, surveillance, democracy, responsibility, how to maintain control of intelligent machines, and conceptual issues regarding machine intelligence. In this chapter we present some challenges in connection with the making of this course, and how these challenges were overcome.
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  • Resultat 1-7 av 7

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