Sökning: WFRF:(Lindström Tobias)
> Ahlm Clas >
Detection and Isola...
Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013
-
- Bergqvist, Joakim (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Forsman, Oscar (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Larsson, Pär (författare)
- Umeå universitet,Institutionen för medicinsk biovetenskap
-
visa fler...
-
- Näslund, Jonas (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
Lilja, Tobias (författare)
-
- Engdahl, Cecilia (författare)
- Umeå universitet,Kemiska institutionen
-
Lindström, Anders (författare)
-
- Gylfe, Åsa (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Ahlm, Clas (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Arcum
-
- Evander, Magnus (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Arktiskt centrum vid Umeå universitet (Arcum),Arcum
-
- Bucht, Göran (författare)
- [ 1 ] CBRN Def & Secur, Swedish Def Res Agcy, SE-90182 Umea, Sweden
-
visa färre...
-
(creator_code:org_t)
- Mary Ann Liebert Inc, 2015
- 2015
- Engelska.
-
Ingår i: Vector Borne and Zoonotic Diseases. - : Mary Ann Liebert Inc. - 1530-3667 .- 1557-7759. ; 15:2, s. 133-140
- Relaterad länk:
-
https://umu.diva-por... (primary) (Raw object)
-
visa fler...
-
http://umu.diva-port...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Mosquito-borne alphaviruses have the potential to cause large outbreaks throughout the world. Here we investigated the causative agent of an unexpected Sindbis virus (SINV) outbreak during August-September, 2013, in a previously nonendemic region of Sweden. Mosquitoes were collected using carbon dioxide-baited CDC traps at locations close to human cases. The mosquitoes were initially screened as large pools by SINV-specific quantitative RT-PCR, and the SINV-positive mosquitoes were species determined by single-nucleotide polymorphism (SNP) analysis, followed by sequencing the barcoding region of the cytochrome oxidase I gene. The proportion of the collected mosquitoes was determined by a metabarcoding strategy. By using novel strategies for PCR screening and genetic typing, a new SINV strain, Lovanger, was isolated from a pool of 1600 mosquitoes composed of Culex, Culiseta, and Aedes mosquitoes as determined by metabarcoding. The SINV-positive mosquito Culiseta morsitans was identified by SNP analysis and sequencing. After whole-genome sequencing and phylogenetic analysis, the SINV Lovanger isolate was shown to be most closely similar to recent Finnish SINV isolates. In conclusion, within a few weeks, we were able to detect and isolate a novel SINV strain and identify the mosquito vector during a sudden SINV outbreak.
Nyckelord
- Barcoding
- Mosquito
- Isolation
- Virus
- Sindbis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Bergqvist, Joaki ...
-
Forsman, Oscar
-
Larsson, Pär
-
Näslund, Jonas
-
Lilja, Tobias
-
Engdahl, Cecilia
-
visa fler...
-
Lindström, Ander ...
-
Gylfe, Åsa
-
Ahlm, Clas
-
Evander, Magnus
-
Bucht, Göran
-
visa färre...
- Artiklar i publikationen
-
Vector Borne and ...
- Av lärosätet
-
Umeå universitet