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Sökning: WFRF:(Liu Ning Qing) > Kungliga Tekniska Högskolan

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Zhigang, Liu, et al. (författare)
  • An MBSE Tool to Support Architecture Design for Spacecraft Electrical Power System
  • 2018
  • Konferensbidrag (refereegranskat)abstract
    • Since difficulty and complexity of spaceflight missions are increasing, current electric systems in the spacecraft become increasingly complicated challenging the spacecraft electrical power system (EPS) development. By using a traditional approach, architectures of EPS are managed by document collections including various reports, documents and data chats. Due to lack of unified visualization of system architectures in such document collections, consistency and traceability among documents, data and related system developers are difficult to be managed, particular in supporting verification of system architectures. This paper presents a model-based systems engineering (MBSE) solution emphasis on domain-specific modeling (DSM), architecture visualization, model transformation and Modelica. The aim of this MBSE tool is to support management of specifications and architecture models, realize architecture-driven system verification & validation of EPS by using Modelica language and visualization of architectural hierarchy inspired by related techniques of Google Earth. Finally, through a case study of EPS, we evaluate the feasibility and effectiveness of our solutions.
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