| 1. |
- Pereira, Rui, 1986, et al.
(författare)
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Metabolic Engineering of Yeast
- 2021
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Ingår i: Metabolic Engineering: Concepts and Applications: Volume 13a and 13b. - : Wiley. ; , s. 689-733
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This chapter focuses on a few examples that can serve as illustrations of how powerful yeast metabolic engineering stands today. Yeast, especially S. cerevisiae, plays an essential role in bioethanol production. Rapid ethanol production by yeast cells makes the fermentation process less susceptible to contamination. Higher alcohols are attractive due to some advantages compared with bioethanol, such as higher energy density, better blending into gasoline, higher octane value, lower hygroscopicity, and less corrosivity. The ethanol production process in the industry is mainly achieved through simultaneous saccharification and fermentation. Production of insulin, by volume the largest pharmaceutical protein produced, has paved the way for a wide use of S. cerevisiae for production of recombinant proteins. Virus like particles are proteins of virus capsid, which are produced by recombinant DNA technology and are important for the development of viral vaccines as they can self-assemble and display similar immunogenic properties as native viruses.
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| 2. |
- Li, Zhen, et al.
(författare)
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Photocatalytic Hydrogen Production
- 2022
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Ingår i: Photo‐ and Electro‐Catalytic Processes. - : John Wiley & Sons. ; , s. 415-483
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Liu, Quanli, 1988, et al.
(författare)
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Modular Pathway Rewiring of Yeast for Amino Acid Production
- 2018
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Ingår i: Methods in Enzymology. - : Elsevier. - 1557-7988 .- 0076-6879. ; , s. 417-439
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Amino acids find various applications in biotechnology in view of their importance in the food, feed, pharmaceutical, and personal care industries as nutrients, additives, and drugs, respectively. For the large-scale production of amino acids, microbial cell factories are widely used and the development of amino acid-producing strains has mainly focused on prokaryotes Corynebacterium glutamicum and Escherichia coli. However, the eukaryote Saccharomyces cerevisiae is becoming an even more appealing microbial host for production of amino acids and derivatives because of its superior molecular and physiological features, such as amenable to genetic engineering and high tolerance to harsh conditions. To transform S. cerevisiae into an industrial amino acid production platform, the highly coordinated and multiple layers regulation in its amino acid metabolism should be relieved and reconstituted to optimize the metabolic flux toward synthesis of target products. This chapter describes principles, strategies, and applications of modular pathway rewiring in yeast using the engineering of L-ornithine metabolism as a paradigm. Additionally, detailed protocols for in vitro module construction and CRISPR/Cas-mediated pathway assembly are provided.
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