SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Ljunggren Osten)
 

Sökning: WFRF:(Ljunggren Osten) > Larsson Tobias E > Gene expression ana...

Gene expression analysis of kidneys from transgenic mice expressing fibroblast growth factor-23.

Marsell, Richard (författare)
Uppsala universitet,Ortopedi
Krajisnik, Tijana (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
Göransson, Hanna (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
visa fler...
Ohlsson, Claes, 1965 (författare)
Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine
Ljunggren, Osten (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
Larsson, Tobias E. (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
Jonsson, Kennet B (författare)
Uppsala universitet,Ortopedi
visa färre...
 (creator_code:org_t)
2007-10-15
2008
Engelska.
Ingår i: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. - : Oxford University Press (OUP). - 1460-2385 .- 0931-0509. ; 23:3, s. 827-33
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • BACKGROUND: Fibroblast growth factor-23 (FGF23), a circulating protein produced in bone, causes decreased renal inorganic phosphate (Pi) reabsorption by reducing the expression of the sodium phosphate cotransporter type 2a (Npt2a). We have previously generated transgenic mice expressing human wild-type (WT) FGF23 under the control of the alpha1 (I) collagen promoter. METHODS: In this study, we performed a large-scale gene expression study of kidneys from FGF23 transgenic mice and WT littermates. Microarray expression data of key transcripts were verified by real-time RT-PCR analysis. RESULTS: Several genes that play a role in Pi regulation revealed decreased expression levels in the transgenic mice, such as Npt2a and Pdzk1, a scaffolding protein known to interact with Npt2a. Importantly, Klotho, a suggested FGF23 receptor cofactor, was the most significantly decreased transcript and alpha2-Na(+)/K(+)-ATPase (Atp1a2), a gene isoform of alpha1-Na(+)/K(+)-ATPase (Atp1a1) which has recently been shown to interact with Klotho and regulate calcium metabolism, was the most increased transcript. In contrast, other genes proposed to regulate Pi levels, such as secreted frizzled-related protein-4 (sFrp4) and Na(+)/H(+) exchanger regulatory factor-1 (Nherf1) revealed no changes. CONCLUSIONS: FGF23 transgenic mice display differentially expressed transcript levels of several genes essential in renal Pi regulation. These findings may lead to further understanding of how FGF23 mediates its actions on renal Pi regulation.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Nyckelord

Animals
Calcium
metabolism
Fibroblast Growth Factors
genetics
metabolism
Gene Expression Profiling
Homeostasis
physiology
Intracellular Signaling Peptides and Proteins
genetics
metabolism
Kidney
metabolism
pathology
Mice
Mice
Transgenic
Oligonucleotide Array Sequence Analysis
Phosphates
metabolism
Phosphoproteins
genetics
metabolism
RNA
Messenger
Sodium-Hydrogen Antiporter
genetics
metabolism
Sodium-Phosphate Cotransporter Proteins
Type IIa
genetics
metabolism
Sodium-Potassium-Exchanging ATPase
genetics
metabolism
MEDICINE
Orthopaedics

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy