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Sökning: WFRF:(Lundberg Kristofer) > Uppsala universitet

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1.
  • Nejdl, Lukas, et al. (författare)
  • Label-free bead-based metallothionein electrochemical immunosensor
  • 2015
  • Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 36:16, s. 1894-1904
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 L/min). In stationary arrangement it is possible to detect MT concentrations up to 30ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 g/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.
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2.
  • Westerling, Lars, 1943- (författare)
  • Interaction of Cylindrical Penetrators with Ceramic and Electromagnetic Armour
  • 2013
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Armoured vehicles have traditionally used steel armour as protection against penetrators such as projectiles and shaped charge jets. The latter produce a thin stretching metal jet, usually of copper, with a tip velocity of about 7-8 km/s. In order to obtain more weight-efficient solutions, there is a search for lighter materials and other protection techniques. In this thesis, ceramic and electromagnetic armours are studied. Ceramic materials are lighter than steel, and their high compressive strength makes them useful as armour materials. Electromagnetic armour consists of two metal plates connected to an electric power supply capable of delivering a strong current pulse. A conductive penetrator passing through both plates is destroyed by the effects of the resulting current.Tests of the ceramic armour materials alumina and boron carbide were performed with reverse impact technique, which signifies that a target assembly (ceramic confined in a metal cylinder) was launched by a gun towards a projectile placed in front of the gun barrel. By this technique yaw was eliminated, but the geometric scale had to be very small. Therefore, we studied scaling laws for ceramic armour through a series of tests with direct impact technique and projectile diameters from 2 to 10 mm. The small scale has the advantage that flash X-ray photography can be used to photograph the projectile inside the ceramic target. The phenomenon of interface defeat or dwell was also demonstrated. It signifies that the ceramic, at least for a short time, can withstand the impact pressure so that the projectile just flows out onto the target surface. A transition velocity, above which dwell does not occur, was determined. Simulations were performed with the continuum-dynamic code Autodyn and by use of a model for the brittle ceramic materials by Johnson and Holmquist. The simulations reasonably well represented the penetration behaviour above the transition velocity. They also did below, if under this condition the ceramic model was forced to remain undamaged.The performance of electromagnetic armour was tested against a shaped charge jet. The jet was registered with shadowgraph flash X-ray photography between the plates and after passing through the plates. The current through the jet and the voltage over the plates were also registered. The current caused heating leading to melting and even vaporization. The magnetic Lorentz force compressed the jet radially, and as this effect increases with decreasing jet radius, instability may arise. Explosions in the compressed regions resulted in a fragmented jet with disk-shaped fragments which are less effective penetrators than the elongated fragments obtained in the absence of current. We also performed a theoretical study, in which the penetrator was subjected to small elastic strains only and the current was constant. The magnetic field was determined by FFT, and the stresses due to the Lorentz force were calculated with a semi-analytical method. The velocity skin effect was demonstrated.
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