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Träfflista för sökning "WFRF:(Magnusson G) srt2:(2005-2009);pers:(Magnusson Karl Erik)"

Sökning: WFRF:(Magnusson G) > (2005-2009) > Magnusson Karl Erik

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1.
  • Bolshakova, A.V., et al. (författare)
  • Comparative analysis of subcellular fractionation methods for revealing a-actinin 1 and a-actinin 4 in A431 cells
  • 2009
  • Ingår i: Cell and Tissue Biology. - : SP MAIK Nauka/Interperiodica. - 1990-519X .- 1990-5203. ; 3:2, s. 188-197
  • Tidskriftsartikel (refereegranskat)abstract
    • a-Actinin 1 and a-actinin 4 are actin-binding proteins with shared structural functions that are responsible for the regulation of several processes in the cell. Based on previous data on the different distribution of these proteins in the nucleus and cytoplasm, we have studied in detail the presence of a-actinin 1 and a-actinin 4 in subcellular fractions in the A431 cells spread on fibronectin. The detection of a-actinins in some particular fractions has been shown to depend on the method of lysis, as well as whether the preliminary low-temperature freezing of cells was used. The application of various fractionation methods has allowed us to conclude that a-actinin 4 is present in all cytoplasmic and nuclear subfractions, whereas, in addition to in the cytoplasm, a-actinin 1 can also be revealed in the nuclear envelope fraction.
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2.
  • Bolshakova, A.V., et al. (författare)
  • The comparative analysis of subcellular fractionation methods for revealing of a-actinin 1 and a-actinin 4 in A431 cells
  • 2009
  • Ingår i: Tsitologiya. - St Petersburg, Russian Federation : Sankt-Peterburgskaya Izdatel'skaya Firma Nauka. - 0041-3771. ; 51:2, s. 122-129
  • Tidskriftsartikel (refereegranskat)abstract
    • a-Actinin 1 and a-actinin 4 belong to a family of actin-binding proteins with shared structural function and regulation of several processes in a cell. Based on previous data on different distribution of these proteins in the nucleus and cytoplasm, we have explored in detail the distribution of a-actinin 1 and a-actinin 4 in subcellular fractions in A431 cells spread on fibronectin. Several methods of subcellular fractionation were used. Complex approach allowed resuming that revealing of a-actinin isoforms in fractions depended on the composition of lysis buffer and preliminary low-temperature freezing of the cells. We have drawn a conclusion that a-actinin 4 can be found in all cytoplasmic and nuclear subfractions, while a-actinin 1 is characterized by cytoplasmic and membrane localization with specificity of its distribution tightly to the nuclear membrane.
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  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
Typ av innehåll
refereegranskat (2)
Författare/redaktör
Magnusson, Karl-... (2)Ta bort avgränsningen
Petukhova, O.A. (2)
Bolshakova, A.V. (2)
Pinaev, G.P. (2)
Lärosäte
Linköpings universitet (2)
Språk
Engelska (2)
Forskningsämne (UKÄ/SCB)
Naturvetenskap (1)
Medicin och hälsovetenskap (1)
År
2009 (2)

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