| 1. |
- Björnsson, H., et al.
(författare)
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Velocities in the plume of the 2010 Eyjafjallajökull eruption
- 2013
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Ingår i: Journal of Geophysical Research. - : American Geophysical Union (AGU). - 0148-0227 .- 2156-2202 .- 2169-897X. ; 118:20, s. 11698-11711
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Tidskriftsartikel (refereegranskat)abstract
- The eruption of the Icelandic volcano Eyjafjallajökull in the spring of 2010 lasted for 39 days with an explosive phase (14-18 April), an effusive phase (18 April to 4 May) and a phase with renewed explosive activity (5-17 May). Images every 5 s from a camera mounted 34 km from the volcano are available for most of the eruption. Applying the maximum cross-correlation method (MCC) on these images, the velocity structure of the eruption cloud has been mapped in detail for four time intervals covering the three phases of the eruption. The results show that on average there are updrafts in one part of the cloud and lateral motion or downdrafts in another. Even within the updraft part, there are alternating motions of strong updrafts, weak updrafts, and downward motion. These results show a highly variable plume driven by intermittent explosions. The results are discussed in the context of integral plume models and in terms of elementary parcel theory. Key Points Velocities in a volcanic cloud based on analysis of image data from the eruption Velocities in the eruption cloud are inhomogeneous and updrafts intermittent Intermittent updrafts are important for the dynamics and the lofting of ash.
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| 2. |
- Blau, Helen M., et al.
(författare)
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Elastic substrates and methods of use in cell manipulation and culture
- 2010
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Patent (populärvet., debatt m.m.)abstract
- Methods are provided for the ex vivo manipulation of cells, stem cells and other reproductive cells, by manipulating the cells in a container or device comprising an elastic substrate, wherein the substrate has an elasticity that mimics the elasticity of a native microenvironment of the cell.
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| 3. |
- Cepeda, D., et al.
(författare)
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CDK-mediated activation of the SCFFBXO28 ubiquitin ligase promotes MYC-driven transcription and tumourigenesis and predicts poor survival in breast cancer
- 2013
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Ingår i: EMBO Molecular Medicine. - : EMBO. - 1757-4676 .- 1757-4684. ; 5:7, s. 999-1018
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Tidskriftsartikel (refereegranskat)abstract
- SCF (Skp1/Cul1/F-box) ubiquitin ligases act as master regulators of cellular homeostasis by targeting key proteins for ubiquitylation. Here, we identified a hitherto uncharacterized F-box protein, FBXO28 that controls MYC-dependent transcription by non-proteolytic ubiquitylation. SCFFBXO28 activity and stability are regulated during the cell cycle by CDK1/2-mediated phosphorylation of FBXO28, which is required for its efficient ubiquitylation of MYC and downsteam enhancement of the MYC pathway. Depletion of FBXO28 or overexpression of an F-box mutant unable to support MYC ubiquitylation results in an impairment of MYC-driven transcription, transformation and tumourigenesis. Finally, in human breast cancer, high FBXO28 expression and phosphorylation are strong and independent predictors of poor outcome. In conclusion, our data suggest that SCFFBXO28 plays an important role in transmitting CDK activity to MYC function during the cell cycle, emphasizing the CDK-FBXO28-MYC axis as a potential molecular drug target in MYC-driven cancers, including breast cancer. FBXO28 is identified as part of a SCF complex acting as a regulator of tumor cell proliferation and an important modifier of MYC function. FBXO28 may be a new prognostic factor in breast cancer and a new potential drug target in MYC- driven tumors.
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| 4. |
- Colombo, Massimo G., et al.
(författare)
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Introduction : Small Business and Networked Innovation: Organizational and Managerial Challenges
- 2012
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Ingår i: Journal of small business management (Print). - 0047-2778 .- 1540-627X. ; 50:2, s. 181-190
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this article is to provide an introduction to the special issue. We briefly consider the organizational and managerial challenges that small and medium-sized enterprises encounter in networked innovation, thereby building a background to the articles included in the special issue. We then present the main findings of these articles and highlight their novel contributions.
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| 5. |
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| 6. |
- Gilbert, Penney M., et al.
(författare)
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A single cell bioengineering approach to elucidate mechanisms of adult stem cell self-renewal
- 2012
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Ingår i: Integrative Biology. - : Oxford University Press (OUP). - 1757-9694 .- 1757-9708. ; 4:4, s. 360-367
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Tidskriftsartikel (refereegranskat)abstract
- The goal of regenerative medicine is to restore form and function to damaged and aging tissues. Adult stem cells, present in tissues such as skeletal muscle, comprise a reservoir of cells with a remarkable capacity to proliferate and repair tissue damage. Muscle stem cells, known as satellite cells, reside in a quiescent state in an anatomically distinct compartment, or niche, ensheathed between the membrane of the myofiber and the basal lamina. Recently, procedures for isolating satellite cells were developed and experiments testing their function upon transplantation into muscles revealed an extraordinary potential to contribute to muscle fibers and access and replenish the satellite cell compartment. However, these properties are rapidly lost once satellite cells are plated in culture. Accordingly, elucidating the role of extrinsic factors in controlling muscle stem cell fate, in particular self-renewal, is critical. Through careful design of bioengineered culture platforms, analysis of specific proteins presented to stem cells is possible. Critical to the success of the approach is single cell analysis, as more rapidly proliferating progenitors may mask the behavior of stem cells that proliferate slowly. Bioengineering approaches provide a potent means of gaining insight into the role of extrinsic factors in the stem cell microenvironment on stem cell function and the mechanisms that control their diverse fates. Ultimately, the multidisciplinary approach presented here will lead to novel therapeutic strategies for degenerative diseases.
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| 7. |
- Gilbert, P. M., et al.
(författare)
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Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
- 2010
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 329:5995, s. 1078-1081
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Tidskriftsartikel (refereegranskat)abstract
- Stem cells that naturally reside in adult tissues, such as muscle stem cells (MuSCs), exhibit robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, we show that substrate elasticity is a potent regulator of MuSC fate in culture. Unlike MuSCs on rigid plastic dishes (similar to 10(6) kilopascals), MuSCs cultured on soft hydrogel substrates that mimic the elasticity of muscle (12 kilopascals) self-renew in vitro and contribute extensively to muscle regeneration when subsequently transplanted into mice and assayed histologically and quantitatively by noninvasive bioluminescence imaging. Our studies provide novel evidence that by recapitulating physiological tissue rigidity, propagation of adult muscle stem cells is possible, enabling future cell-based therapies for muscle-wasting diseases.
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| 8. |
- Holgersson, G., et al.
(författare)
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Molecular profiling using tissue microarrays as a tool to identify predictive biomarkers in laryngeal cancer treated with radiotherapy
- 2010
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Ingår i: Cancer Genomics & Proteomics. - 1109-6535 .- 1790-6245. ; 7:1, s. 1-7
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To explore the usefulness of the expression of five potential cancer biomarkers in predicting outcome in patients with laryngeal cancer. Materials and Methods: In the present study, the Swedish National Cancer Registry databases were used to identify patients with laryngeal cancer diagnosed during the years 1978-2004 in the Uppsala-Örebro region and treated with radiotherapy. The expression of Ki-67, MutS homolog 2, (MSH2), p53, B-cell CLL/lymphoma 2 (Bcl-2) and cyclin D1 in the cancer cells was assessed immunohistochemically using tissue microarrays (TMAs) and its predictve value on survival and relapse was analyzed using Cox regression models. Results: A total of 39 patients were included in the present study. Nuclear MSH2 staining was statistically significantly correlated to Ki-67 expression (p=0.022). However, univariate and multivariate Cox analyses showed no statistically significant association between the expression of the investigated biomarkers and overall survival or relapse. Conclusion: The present exploratory study does not show any significant predictive value of the biomarkers examined with respect to survival or relapse. However, with larger patient cohorts, we believe that protein profiling using TMAs and immunohistochemistry is a feasible strategy for prognostic and predictive biomarker screening in laryngeal cancer.
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| 9. |
- Lindfeldt, Erik G., et al.
(författare)
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Strategies for a road transport system based on renewable resources : The case of an import-independent Sweden in 2025
- 2010
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Ingår i: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 87:6, s. 1836-1845
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Tidskriftsartikel (refereegranskat)abstract
- When discussing how society can decrease greenhouse gas emissions, the transport sector is often seen as posing one of the most difficult problems. In addition, the transport sector faces problems related to security of supply. The aim of this paper is to present possible strategies for a road transport system based on renewable energy sources and to illustrate how such a system could be designed to avoid dependency on imports, using Sweden as an example. The demand-side strategies considered include measures for decreasing the demand for transport, as well as various technical and non-technical means of improving vehicle fuel economy. On the supply side, biofuels and synthetic fuels produced from renewable electricity are discussed. Calculations are performed to ascertain the possible impact of these measures on the future Swedish road transport sector. The results underline the importance of powerful demand-side measures and show that although biofuels can certainly contribute significantly to an import-independent road transport sector, they are far from enough even in a biomass-rich country like Sweden. Instead, according to this study, fuels based on renewable electricity will have to cover more than half of the road transport sector's energy demand.
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| 10. |
- Magnusson, Klas E. G., et al.
(författare)
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A batch algorithm using iterative application of the Viterbi algorithm to track cells and construct cell lineages
- 2012
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Ingår i: Proceedings - International Symposium on Biomedical Imaging. - : Institute of Electrical and Electronics Engineers. - 9781457718588 ; , s. 382-385
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Konferensbidrag (refereegranskat)abstract
- Advances in microscope hardware in the last couple of decades have made it possible to acquire large data sets with image sequences of living cells grown in cell culture. This has led to a demand for automated ways of analyzing the acquired images. This article presents a new algorithm for tracking cells and constructing cell lineages in such image sequences. The algorithm uses information from the entire sequence to make local decisions about cell tracks and can therefore make more robust decisions than algorithms that process the data sequentially. It also incorporates image-based likelihoods of cell division and cell death into the tracking, without having to resort to separate detection algorithms or post processing of tracks. The algorithm consists of a scoring function to rank tracks and an iterative algorithm that searches for the highest scoring tracks, in a computationally efficient way, using the Viterbi algorithm.
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