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Träfflista för sökning "WFRF:(Marie Rodolphe) "

Sökning: WFRF:(Marie Rodolphe)

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2.
  • Gunnarsson, Anders, et al. (författare)
  • Single-molecule Detection and Mismatch Discrimination of Unlabeled DNA Targets
  • 2008
  • Ingår i: Nano Letters. - The American Chemical Society (ACS). - 1530-6992. ; 8:1, s. 183-188
  • Tidskriftsartikel (refereegranskat)abstract
    • We report on a single-molecule readout scheme on total internal reflection fluorescence microscopy (TIRFM) demonstrating a detection limit in the low fM regime for short (30-mer) unlabeled DNA strands. Detection of unlabeled DNA targets is accomplished by letting them mediate the binding of suspended fluorescently labeled DNA-modified small unilamellar vesicles (Ø approximately 100 nm) to a DNA-modified substrate. On top of rapid and sensitive detection, the technique is also shown capable of extracting kinetics data from statistics of the residence time of the binding reaction in equilibrium, that is, without following neither the rate of binding upon injection nor release upon rinsing. The potential of this feature is demonstrated by discriminating a single mismatch from a fully complementary sequence. The success of the method is critically dependent on a surface modification that provides sufficiently low background. This was achieved through self-assembly of a biotinylated copolymer, Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) on a silicon dioxide surface, followed by subsequent addition of streptavidin and biotinylated DNA. The proposed detection scheme is particularly appealing due to the simplicity of the sensor, which relies on self-assembly principles and conventional TIRFM. Therefore, we foresee a great potential of the concept to serve as an important component in future multiplexed sensing schemes. This holds in particular true in cases when information about binding kinetics is valuable, such as in single nucleotide polymorphism diagnostics.
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3.
  • Marie, Rodolphe, et al. (författare)
  • A generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures
  • 2007
  • Ingår i: Biointerphases. - AVS. - 1934-8630. ; 2:1, s. 49-55
  • Tidskriftsartikel (refereegranskat)abstract
    • A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. (C) 2007 American Vacuum Society.
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4.
  • Marie, Rodolphe, et al. (författare)
  • Immobilisation of DNA to polymerised SU-8 photoresist
  • 2006
  • Ingår i: Biosensors & Bioelectronics. - Elsevier. - 1873-4235. ; 21:7, s. 1327-1332
  • Tidskriftsartikel (refereegranskat)abstract
    • SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.
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5.
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6.
  • Marie, Rodolphe, et al. (författare)
  • Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding
  • 2006
  • Ingår i: Langmuir. - The American Chemical Society (ACS). - 0743-7463. ; 22:24, s. 10103-10108
  • Tidskriftsartikel (refereegranskat)abstract
    • By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.
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7.
  • McGinn, Steven, et al. (författare)
  • New technologies for DNA analysis : a review of the READNA Project
  • 2016
  • Ingår i: New Biotechnology. - 1871-6784 .- 1876-4347. ; 33:3, s. 311-330
  • Forskningsöversikt (övrigt vetenskapligt)abstract
    • The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively.
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8.
  • McGinn, Steven, et al. (författare)
  • New Technologies for DNA analysis-A review of the READNA Project.
  • 2015
  • Ingår i: New Biotechnology. - Elsevier. - 1876-4347.
  • Forskningsöversikt (refereegranskat)abstract
    • The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.
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9.
  • Tenje, Maria, et al. (författare)
  • Rendering SU-8 hydrophilic to facilitate use in micro channel fabrication
  • 2004
  • Ingår i: Journal of Micromechanics and Microengineering. - IOP Publishing. - 0960-1317. ; 14:12, s. 1614-1617
  • Tidskriftsartikel (refereegranskat)abstract
    • A simple method is presented to reduce the contact angle of the photo-resist SU-8. A low contact angle is valuable in micro total analysis systems for the fabrication of micro channels where the capillary pressure is linearly related to the cosine of the contact angle, θ. If the surface of the channel is hydrophilic, the capillary pressure can be used as the only means to direct the liquid through the channels and the need for external pumps can be avoided. This is very useful, especially in the fabrication of devices for 'lab-on-a-chip' where it is important to keep the design as simple as possible. A commonly used technique for releasing structures fabricated on Si wafers is to use a sacrificial Cr layer. It is shown that the contact angle of SU-8 decreases by 40° after etching this layer. A further reduction in contact angle is desirable and can be achieved by treating the sample with ethanolamine at 50 °C for only 10 min. The resulting contact angle is 23° ± 7°. Using a wet chemical treatment, a selective change in contact angle between different areas of a micro channel system can be achieved, without the need to involve different materials in the fabrication process.
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