| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Patrinopoulou, N., et al.
(författare)
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Metropolis II: Investigating the Future Shape of Air Traffic Control in Highly Dense Urban Airspace
- 2022
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Ingår i: 2022 30TH MEDITERRANEAN CONFERENCE ON CONTROL AND AUTOMATION (MED). - : IEEE. - 9781665406734 ; , s. 649-655
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Konferensbidrag (refereegranskat)abstract
- Metropolis II aims to provide insights in what is needed to enable high-density urban air operations. It does this by investigating the foundation for U-space U3/U4 services. The final goal is to provide a unified approach for strategic deconfliction, tactical deconfliction, and dynamic capacity management. Highly-dense operations in constrained urban airspace will likely require a degree of complexity that does not exist in modern-day air traffic management. The expected high traffic demand will require a shared use of the airspace instead of assigning exclusive use of blocks of the airspace to some flights. A unified approach for traffic management is needed because at high-densities, airspace design, flight planning, and separation management become increasingly interdependent. Metropolis II builds upon the results of the first Metropolis project. Three concepts with a varying degree of centralisation will be compared using simulations. (1) The centralised concept will take a global approach for separation management. (2) The decentralised concept aims to give the individual agents separation responsibility. (3) The hybrid concept tries to combine a centralised strategic planning agent with a robust tactical separation strategy.
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