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Allergome-wide pept...
Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy
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- Mikus, Maria (author)
- Karolinska Institutet,KTH Royal Institute of Technology,Karolinska Institute,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Zandian, Arash (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab
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- Sjöberg, Ronald (author)
- KTH Royal Institute of Technology,KTH,Skolan för kemi, bioteknologi och hälsa (CBH)
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- Hamsten, C (author)
- Karolinska Institutet
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- Forsström, B (author)
- KTH Royal Institute of Technology
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- Andersson, Morgan (author)
- Skåne University Hospital
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- Greiff, Lennart (author)
- Lund University,Lunds universitet,Öron-, näs- och halssjukdomar, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Vårdrelaterade infektioner hos svårt och kristiskt sjuka,Forskargrupper vid Lunds universitet,Otorhinolaryngology (Lund),Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Health care related infections in seriously and critically ill patients,Lund University Research Groups,Skåne University Hospital
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- Uhlén, Mathias (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Albanova VinnExcellence Center for Protein Technology, ProNova,Systembiologi
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- Levin, Mattias (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Peter (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- van Hage, M (author)
- Karolinska University Hospital
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- Ohlin, Mathias, 1984- (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Ohlin, Mats (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Mosby Inc. 2020
- 2020
- English.
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In: Journal of Allergy and Clinical Immunology. - : Mosby Inc.. - 0091-6749 .- 1097-6825.
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Abstract
Subject headings
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- Background: The interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking of receptors on effector cells. Antibodies of other isotypes may modulate such a reaction. Receptor crosslinking requires binding of antibodies to multiple epitopes on the allergen. Limited information is available on the complexity of the epitope structure of most allergens. Objectives: We sought to allow description of the complexity of IgE, IgG4, and IgG epitope recognition at a global, allergome-wide level during allergen-specific immunotherapy (AIT). Methods: We generated an allergome-wide microarray comprising 731 allergens in the form of more than 172,000 overlapping 16-mer peptides. Allergen recognition by IgE, IgG4, and IgG was examined in serum samples collected from subjects undergoing AIT against pollen allergy. Results: Extensive induction of linear peptide-specific Phl p 1– and Bet v 1–specific humoral immunity was demonstrated in subjects undergoing a 3-year-long AIT against grass and birch pollen allergy, respectively. Epitope profiles differed between subjects but were largely established already after 1 year of AIT, suggesting that dominant allergen-specific antibody clones remained as important contributors to humoral immunity following their initial establishment during the early phase of AIT. Complex, subject-specific patterns of allergen isoform and group cross-reactivities in the repertoires were observed, patterns that may indicate different levels of protection against different allergen sources. Conclusions: The study highlights the complexity and subject-specific nature of allergen epitopes recognized following AIT. We envisage that epitope deconvolution will be an important aspect of future efforts to describe and analyze the outcomes of AIT in a personalized manner.
Subject headings
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Allergen
- IgE
- IgG
- IgG4
- allergen-specific immunotherapy
- antibody
- epitope
- linear epitope
- peptide microarray
- Allergen
- allergen-specific immunotherapy
- antibody
- epitope
- IgE
- IgG
- linear epitope
- peptide microarray
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Mikus, Maria
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Zandian, Arash
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Sjöberg, Ronald
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Hamsten, C
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Forsström, B
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Andersson, Morga ...
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show more...
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Greiff, Lennart
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Uhlén, Mathias
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Levin, Mattias
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Nilsson, Peter
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van Hage, M
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Ohlin, Mathias, ...
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Ohlin, Mats
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Organic Chemistr ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Journal of Aller ...
- By the university
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Royal Institute of Technology
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Lund University
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Karolinska Institutet