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Träfflista för sökning "WFRF:(Mir Kalim) "

Sökning: WFRF:(Mir Kalim)

  • Resultat 1-6 av 6
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1.
  • Beech, Jason P., et al. (författare)
  • Sample preparation for single-cell whole chromosome analysis
  • 2012
  • Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - Chemical and Biological Microsystems Society. - 9780979806452 ; s. 998-999
  • Konferensbidrag (refereegranskat)abstract
    • In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.
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2.
  • Freitag, C., et al. (författare)
  • Meandering nanochannels for imaging of ultra-long DNA molecules
  • 2011
  • Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 3, s. 1758-1760
  • Konferensbidrag (refereegranskat)abstract
    • A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.
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3.
  • Fritzsche, Joachim, et al. (författare)
  • A lipid-based passivation scheme for nanofluidics
  • 2012
  • Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - Chemical and Biological Microsystems Society. - 9780979806452 ; s. 1876-1878
  • Konferensbidrag (refereegranskat)abstract
    • Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.
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4.
  • McGinn, Steven, et al. (författare)
  • New technologies for DNA analysis : a review of the READNA Project
  • 2016
  • Ingår i: New Biotechnology. - 1871-6784 .- 1876-4347. ; 33:3, s. 311-330
  • Forskningsöversikt (övrigt vetenskapligt)abstract
    • The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively.
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5.
  • McGinn, Steven, et al. (författare)
  • New Technologies for DNA analysis-A review of the READNA Project.
  • 2015
  • Ingår i: New Biotechnology. - Elsevier. - 1876-4347.
  • Forskningsöversikt (refereegranskat)abstract
    • The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.
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6.
  • Persson, Fredrik, et al. (författare)
  • Lipid-Based Passivation in Nanofluidics
  • 2012
  • Ingår i: Nano Letters. - The American Chemical Society (ACS). - 1530-6992. ; 12:5, s. 2260-2265
  • Tidskriftsartikel (refereegranskat)abstract
    • Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.
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  • Resultat 1-6 av 6
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