| 1. |
- Momeni, Naghi, et al.
(författare)
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A novel blood-based biomarker for detection of autism spectrum disorders
- 2012
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Ingår i: Translational Psychiatry. - : Nature Publishing Group. - 2158-3188. ; 2
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Tidskriftsartikel (refereegranskat)abstract
- Autism Spectrum Disorders (ASD) are classified as neurological developmental disorders. Several studies have been carried out to find a candidate biomarker linked to development of these disorders, but up to date no reliable biomarker is available. Mass spectrometry techniques have been used for protein profiling of blood plasma of children with such disorders in order to identify proteins/peptides which may be used as biomarkers for detection of the disorders. Three differentially expressed peptides with mass charged (m/z) values of 2,020 ± 1, 1,864 ± 1, and 1,978 ± 1 Da in heparin plasma of children with ASD which were significantly changed as compared to the peptide pattern of the non-ASD control group are reported here. This novel set of biomarkers allows for a reliable blood based diagnostic tool that may be used in diagnosis and potentially, in prognosis of ASD.
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| 2. |
- Momeni, Naghi, et al.
(författare)
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Alterations of prolyl endopeptidase activity in the plasma of children with autistic spectrum disorders
- 2005
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Ingår i: BMC Psychiatry. - 1471-244X. ; 5:27
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Prolyl Endopeptidase (PEP, EC 3.4.21.26), a cytosolic endopeptidase, hydrolyses peptide bonds on the carboxyl side of proline residue in proteins with a relatively small molecular weight. It has been shown that altered PEP activity is associated with various psychological diseases such as schizophrenia, mania and depression. Autistic Spectrum Disorders (ASD) are neuropsychiatric and behavioural syndromes affecting social behaviours and communication development. They are classified as developmental disorders. The aim of this study was to examine the hypothesis that PEP activity is also associated with ASDs. METHODS: Fluorometric assay was used to measure PEP activity in EDTA plasma in children with ASD (n = 18) aged 4-12 years (mean +/- SD: 7.9 +/- 2.5). These results were then compared to PEP activity in a control group of non-ASD children (n = 15) aged 2-10 years (mean +/- SD: 6.4 +/- 2.2). RESULTS: An alteration in PEP activity was found in the children with ASD compared to the control group. There was much greater variation of PEP activity in the group of ASD children when compared to the controls (SD= 39.9 and SD 9.6, respectively). This variation was significant (p < 0.0005), although the mean level of PEP activity in the group of ASD children was slightly higher than in the control group (124.4 and 134.1, respectively). CONCLUSION: Our preliminary finding suggests a role for PEP enzyme in the pathophysiology of autism but further research should be conducted to establish its role in the aetiology of psychiatric and neurological disorders, including autism and related spectrum disorders.
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| 3. |
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| 4. |
- Momeni, Naghi, et al.
(författare)
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CCD-camera based capillary chemiluminescent detection of retinol binding protein
- 1999
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Ingår i: Analytica Chimica Acta. - 0003-2670. ; 387:1, s. 21-27
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Tidskriftsartikel (refereegranskat)abstract
- A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml−1 and 11 ng ml−1 with a coefficient of variation of 5–11% (n=6) over this range.
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| 5. |
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| 6. |
- Momeni, Naghi, et al.
(författare)
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High complement factor I activity in the plasma of children with autism spectrum disorders
- 2012
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Ingår i: Autism Research and Treatment. - : Hindawi Publishing Corporation. - 2090-1925 .- 2090-1933.
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Tidskriftsartikel (refereegranskat)abstract
- Autism spectrum disorders (ASDs) are neurodevelopmental and behavioural syndromes affecting social orientation, behaviour, and communication that can be classified as developmental disorders. ASD is also associated with immune system abnormality. Immune system abnormalities may be caused partly by complement system factor I deficiency. Complement factor I is a serine protease present in human plasma that is involved in the degradation of complement protein C3b, which is a major opsonin of the complement system. Deficiency in factor I activity is associated with an increased incidence of infections in humans. In this paper, we show that the mean level of factor I activity in the ASD group is significantly higher than in the control group of typically developed and healthy children, suggesting that high activity of complement factor I might have an impact on the development of ASD.
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