| 1. |
- Barker, Dean, 1977, et al.
(författare)
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Jacob and Martin: Developing digital technology competence in physical education teacher education
- 2016
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Ingår i: Digital Technologies and Learning in Physical Education. - Abingdon, Oxon ; New York, NY : Routledge, 2016. | : Routledge. - 9781315670164 - 9781138947283 - 9781138947290 ; , s. 231-246
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This chapter provides an illustration of how digital technologies (DTs) are experienced by Physical Education Teacher Education (PETE) students. The illustration is based on the reflections of two students at the University of Gothenburg, Sweden. The students received an assignment that involved demonstrating how a specific DT could be implemented. Three perspectives of the practitioners' experiences are provided. A Deweyan perspective shows how the students and their situations are transformed by DTs. A Foucauldian perspective focuses on the regulating aspects of technology. An applied Information Technology perspective demonstrates how DTs become part of the social practices of physical education. © 2017 Ashley Casey, Victoria A. Goodyear and Kathleen M. Armour. All rights reserved.
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| 2. |
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| 3. |
- Borodina, I., et al.
(författare)
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Metabolic engineering of streptomyces
- 2009
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Ingår i: The Metabolic Pathway Engineering Handbook: Fundamentals. - 9781439802977 ; , s. 24-1-24-30
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Some of the common soil microorganisms are actinomycetes, Gram-positive bacteria with high GC content. Because of their mycelial habit they were initially believed to be fungi, which reected in their name (mucus (lat.) means fungus). In 1939, one year before rediscovery of penicillin by Florey and Chain, soil microbiologist Waksman has set his lab on a quest for new antimicrobial drugs. From the previous studies he knew that actinomycetes can inhibit the growth of other soil bacteria through secretion of bioactive compounds, which he named “antibiotics” (anti (lat.) against, bio (lat.) life). Systematic search for antibiotics produced by actinomycetes resulted in the discovery of actinomycin (1940), clavacin, and streptothricin (1942), all of them sadly turned out to be toxic in animal tests. In 1943 Waksman’s student Schatz isolated streptomycin-producing strain of Streptomyces griseus.1 Streptomycin was not particularly toxic to animals and humans, but remarkably was the rst compound active against tuberculosis bacteria. Many pharmaceutical companies and research laboratories started to collect soil samples from all over the world in search of antibiotics-producing organisms. Most of the discoveries were made in the rst ten years of the “hunt,” the larger part involved Streptomyces species. Streptomyces is a genus in the genera of actinomycetes, many of these bacteria produce volatile compounds that give the earth its characteristic odor. Streptomyces proved to be an excellent source of secondary metabolites, including antibiotics, anticancerous agents, antihelmintic drugs, and other useful compounds (Table 24.1). At present more than half of antibiotics in clinical use are produced in Streptomyces species.
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| 4. |
- Chen, Yu, 1990, et al.
(författare)
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Genome-Scale Metabolic Modeling from Yeast to Human Cell Models of Complex Diseases: Latest Advances and Challenges
- 2019
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1940-6029 .- 1064-3745. ; 2049, s. 329-345
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Genome-scale metabolic models (GEMs) are mathematical models that enable systematic analysis of metabolism. This modeling concept has been applied to study the metabolism of many organisms including the eukaryal model organism, the yeast Saccharomyces cerevisiae, that also serves as an important cell factory for production of fuels and chemicals. With the application of yeast GEMs, our knowledge of metabolism is increasing. Therefore, GEMs have also been used for modeling human cells to study metabolic diseases. Here we introduce the concept of GEMs and provide a protocol for reconstructing GEMs. Besides, we show the historic development of yeast GEMs and their applications. Also, we review human GEMs as well as their uses in the studies of complex diseases.
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| 5. |
- Chen, Yu, 1990, et al.
(författare)
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Proteome Constraints in Genome-Scale Models
- 2021
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Ingår i: Metabolic Engineering: Concepts and Applications: Volume 13a and 13b. - : Wiley. ; 13, s. 137-152
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Genome-scale metabolic models (GEMs) describe the stoichiometry of all reactions in the cellular metabolic network, and at the same time associate the reactions to the enzymes that catalyze them. This chapter discusses proteome constraints followed by examples on how one particular type of cellular constraint, namely a proteome constraint, is a powerful approach to improve the predictive strength of GEMs. Cells operate under myriad constraints that govern their phenotypes and functioning. A fundamental constraint in the context of metabolism is the conservation of mass and energy. The chapter addresses the recently developed approach GECKO to illustrate how proteome constraints can be integrated into a GEM in a coarse-grained manner. Coarse-grained approaches as GECKO provide a straightforward platform to integrate proteome constraints. Contrasting with the coarse-grained integration, fine-tuned approaches tend to explicitly integrate biological processes into a GEM, example protein synthesis process.
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| 6. |
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| 7. |
- Holland, Petter, 1985, et al.
(författare)
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Coupling cell division to metabolic pathways through transcription
- 2018
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Ingår i: Encyclopedia of Bioinformatics and Computational Biology: ABC of Bioinformatics. ; 1-3, s. 74-93
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Cellular growth is ensured by alternation of DNA duplication and cell division cycles. This alternation is coordinated by the interplay between enzymatic activities, called kinases, and transcription factors, to keep the cell cycle timing. Here we investigate whether transcription factors may serve as hubs connecting multi-scale cellular networks. A variant of chromatin immunoprecipitation, called ChIP-exo, was performed to identify targets of Forkhead (Fkh) transcription factors across the budding yeast genome. Data analyses indicate that the Fkh-mediated transcriptional program may activate metabolic pathways and synchronize kinase activities to guarantee alternation of DNA duplication and cell division, thereby a timely cell’s cycling.
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| 8. |
- Huang, Mingtao, 1984, et al.
(författare)
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High-throughput microfluidics for the screening of yeast libraries
- 2018
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Ingår i: Synthetic Metabolic Pathways. - New York, NY : Humana Press. - 9781493972944 ; 1671, s. 307-317
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Bokkapitel (refereegranskat)abstract
- Cell factory development is critically important for efficient biological production of chemicals, biofuels, and pharmaceuticals. Many rounds of the Design–Build–Test–Learn cycles may be required before an engineered strain meeting specific metrics required for industrial application. The bioindustry prefer products in secreted form (secreted products or extracellular metabolites) as it can lower the cost of downstream processing, reduce metabolic burden to cell hosts, and allow necessary modification on the final products, such as biopharmaceuticals. Yet, products in secreted form result in the disconnection of phenotype from genotype, which may have limited throughput in the Test step for identification of desired variants from large libraries of mutant strains. In droplet microfluidic screening, single cells are encapsulated in individual droplet and enable high-throughput processing and sorting of single cells or clones. Encapsulation in droplets allows this technology to overcome the throughput limitations present in traditional methods for screening by extracellular phenotypes. In this chapter, we describe a protocol/guideline for high-throughput droplet microfluidics screening of yeast libraries for higher protein secretion. This protocol can be adapted to screening by a range of other extracellular products from yeast or other hosts.
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| 9. |
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| 10. |
- Kumar, Rahul, 1978, et al.
(författare)
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Systems biology: Developments and applications
- 2014
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Ingår i: Molecular Mechanisms in Yeast Carbon Metabolism. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 9783642550133 ; , s. 83-96
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- © Springer-Verlag Berlin Heidelberg 2014. All rights are reserved. Systems biology relies on systems theory concepts and is applicable to both fundamental studies of cellular biology as well as applied research such as metabolic engineering. In this chapter, we map the context of systems biology developments and highlight its contribution in understanding the yeast carbon metabolism. Systems biology not only contributes towards the global overview of metabolism but also in combination with an integrative analysis approach facilitates the elucidation of molecular mechanisms. In particular we discuss the role of systems biology in unraveling the molecular details concerning glucose and galactose metabolism. In conclusion, this chapter provides an overview of the progress and impact of systems biology in carbon metabolism.
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