Sökning: WFRF:(Nilsson Lennart 1942 )
> Balkhed Östholm Åse 1972 >
Multiplex PCR ampli...
Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae
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- Monstein, Hans-Jurg, 1946- (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Molekylärbiologiska tekniklaboratoriet
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- Balkhed Östholm, Åse, 1972- (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk mikrobiologi,Infektionskliniken i Östergötland
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Nilsson, MV (författare)
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Nilsson, M (författare)
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- Dornbusch, Kathrine, 1942- (författare)
- Linköpings universitet,Hälsouniversitetet,Klinisk mikrobiologi
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- Nilsson, Lennart, 1949- (författare)
- Linköpings universitet,Hälsouniversitetet,Klinisk mikrobiologi
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(creator_code:org_t)
- Wiley, 2007
- 2007
- Engelska.
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Ingår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley. - 0903-4641 .- 1600-0463. ; 115:12, s. 1400-1408
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Extended-spectrum β-lactamases (ESBLs) are often mediated by bla-SHV, blaTEM and blaCTX-M genes in Enterobacteriaceae and other Gram-negative bacteria. Numerous molecular typing methods, including PCR-based assays, have been developed for their identification. To reduce the number of PCR amplifications needed we have developed a multiplex PCR assay which detects and discriminates between bla-SHV, blaTEM and blaCTX-M PCR amplicons of 747, 445 and 593 bp, respectively. This multiplex PCR assay allowed the identification of bla-SHV, blaTEM and blaCTX-M genes in a series of clinical isolates of Enterobacteriaceae with previously characterised ESBL phenotype. The presence of blaSHV, blaTEM and blaCTX-M genes was confirmed by partial DNA sequence analysis. Apparently, the universal well-established CTX-M primer pair used here to reveal plasmid-encoded blaCTX-M genes would also amplify the chromosomally located K-1 enzyme gene in all Klebsiella oxytoca strains included in the study.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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