| 1. |
- Alm Rosenblad, Magnus, 1957, et al.
(författare)
-
Detection of signal recognition particle (SRP) RNAs in the nuclear ribosomal internal transcribed spacer 1 (ITS1) of three lineages of ectomycorrhizal fungi (Agaricomycetes, Basidiomycota)
- 2016
-
Ingår i: MycoKeys. - : Pensoft Publishers. - 1314-4057 .- 1314-4049. ; 13, s. 21-33
-
Tidskriftsartikel (refereegranskat)abstract
- During a routine scan for Signal Recognition Particle (SRP) RNAs in eukaryotic sequences, we surprisingly found in silico evidence in GenBank for a 265-base long SRP RNA sequence in the ITS1 region of a total of 11 fully identified species in three ectomycorrhizal genera of the Basidiomycota (Fungi): Astraeus, Russula, and Lactarius. To rule out sequence artifacts, one specimen from a species indicated to have the SRP RNA-containing ITS region in each of these genera was ordered and re-sequenced. Sequences identical to the corresponding GenBank entries were recovered, or in the case of a non-original but conspecific specimen differed by three bases, showing that these species indeed have an SRP RNA sequence incorporated into their ITS1 region. Other than the ribosomal genes, this is the first known case of non-coding RNAs in the eukaryotic ITS region, and it may assist in the examination of other types of insertions in fungal genomes.
|
|
| 2. |
- Sepehri, Sobhan, 1986, et al.
(författare)
-
Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay
- 2019
-
Ingår i: Biosensors-Basel. - : MDPI AG. ; 9:3
-
Tidskriftsartikel (refereegranskat)abstract
- The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.
|
|
| 3. |
- Ahrentorp, Fredrik, et al.
(författare)
-
Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
- 2017
-
Ingår i: Journal of Magnetism and Magnetic Materials. - : Elsevier BV. - 0304-8853 .- 1873-4766. ; 427, s. 14-18
-
Tidskriftsartikel (refereegranskat)abstract
- We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 mu m. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 mu L) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.
|
|
| 4. |
- Sepehri, Sobhan, 1986, et al.
(författare)
-
Homogeneous Differential Magnetic Assay
- 2019
-
Ingår i: Acs Sensors. - : American Chemical Society (ACS). - 2379-3694. ; 4:9, s. 2381-2388
-
Tidskriftsartikel (refereegranskat)abstract
- Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.
|
|
| 5. |
- Olsson, Mattias, 1975, et al.
(författare)
-
Sorption of Pu(III-IV) onto TiO2: A Preliminary Study
- 2003
-
Ingår i: Materials Research Society Symposium Proceedings. - 155899694X ; 757, s. 517-522
-
Konferensbidrag (refereegranskat)abstract
- A preliminary study of the sorption of reduced plutonium (III-IV) onto TiO2 has been done under anoxic conditions at varying pH and ionic strength. The first approach was to study tetravalent Pu, but the sorption decreases with time in this case. This is probably due to disproportionation of Pu(IV) at the TiO2 surface, which then acts as a catalyst. A further observation that supports this explanation is that the sorption of Pu(IV) onto the walls of the experimental equipment is in some cases higher than the sorption of (disproportioned) Pu onto the oxide.Pu(III) sorption onto the oxide was also investigated and showed similarities with the previously investigated sorption of Pm(III) in a similar system.
|
|
| 6. |
- Svane, Maria, 1957, et al.
(författare)
-
On-Line Chemical Analysis of Individual Alkali-Containing Aerosol Particles by Surface Ionization Combined with Time-of-Flight Mass Spectrometry
- 2009
-
Ingår i: Aerosol Science and Technology. - : Informa UK Limited. - 0278-6826 .- 1521-7388. ; 43:7, s. 653-661
-
Tidskriftsartikel (refereegranskat)abstract
- An aerosol mass spectrometer for measurements of the alkali metal content in individual submicron aerosol particles is presented. The instrument combines surface ionization of individual particles on a hot platinum surface with orthogonal acceleration time-of-flight mass spectrometry. The instrument simultaneously provides the content of different alkali metal elements in single particles with high sensitivity. The instrument is characterized in laboratory experiments, and determination of the alkali metal content is demonstrated for particle diameters of 50-500 nm. The technique is demonstrated in ambient air measurements at an urban background site, and sea spray particles and particles originating from biomass burning are identified based on their content of sodium and potassium. Possible further improvements and applications of the technique are discussed.
|
|
| 7. |
|
|
| 8. |
- Dasandi, N., et al.
(författare)
-
The inclusion of health in major global reports on climate change and biodiversity
- 2022
-
Ingår i: Bmj Global Health. - : BMJ. - 2059-7908. ; 7:6
-
Tidskriftsartikel (refereegranskat)abstract
- This article argues that human health has become a key consideration in recent global reports on climate change and biodiversity produced by various international organisations; however, greater attention must be given to the unequal health impacts of climate change and biodiversity loss around the world and the different health adaptation measures that are urgently required.
|
|
| 9. |
- Montelius, Mikael, 1979, et al.
(författare)
-
Multiparametric MRI with spatiotemporal evaluation reveals potential therapy response biomarkers for 177Lu-octreotate therapy of mice with human neuroendocrine tumor
- 2017
-
Ingår i: ISMRM 25th Annual Meeting. 22-27 April 2017, Honolulu, Hawaii, USA.
-
Konferensbidrag (refereegranskat)abstract
- Tissue parameters derived from multiparametric MRI were evaluated as potential imaging biomarkers for therapy response assessment in mice with human neuroendocrine tumor treated with 177Lu-octreotate. Animals were imaged before and repeatedly after 177Lu-octreotate treatment, using T2w, IVIM-DWI, DCE-MRI, T1- and T2*-mapping techniques. MR-parameters were evaluated regionally and longitudinally, and quantitative proteomics was used to evaluate underlying biological response in central and peripheral tumor separately. Several MR-parameters showed strong correlation with tumor response, as verified by MRI-based tumor volume measurements, but also with proteins associated with radiobiological effects on tumor tissue. Spatial and temporal evaluation increased sensitivity of the methods.
|
|
| 10. |
|
|
