| 2. |
- Ferreira, Monica E., et al.
(författare)
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WD40 Domain Divergence Is Important for Functional Differences between he Fission Yeast Tup11 and Tup12 Co-Repressor Proteins
- 2010
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 5:6
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Tidskriftsartikel (refereegranskat)abstract
- We have previously demonstrated that subsets of Ssn6/Tup target genes ave distinct requirements for the Schizosaccharomyces pombe homologs of he Tup1/Groucho/TLE co-repressor proteins, Tup11 and Tup12. The very igh level of divergence in the histone interacting repression domains f the two proteins suggested that determinants distinguishing Tup11 and up12 might be located in this domain. Here we have combined hylogenetic and structural analysis as well as phenotypic haracterization, under stress conditions that specifically require up12, to identify and characterize the domains involved in up12-specific action. The results indicate that divergence in the epression domain is not generally relevant for Tup12-specific function. nstead, we show that the more highly conserved C-terminal WD40 repeat omain of Tup12 is important for Tup12-specific function. Surface amino cid residues specific for the WD40 repeat domain of Tup12 proteins in ifferent fission yeasts are clustered in blade 3 of the propeller-like tructure that is characteristic of WD40 repeat domains. The Tup11 and up12 proteins in fission yeasts thus provide an excellent model system or studying the functional divergence of WD40 repeat domains.
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| 3. |
- Nilsson, Mats E., et al.
(författare)
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Perceptual validation of auralized road traffic noise
- 2011
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Ingår i: Proceedings of Inter-Noise 2011. - Osaka, Japan : The Institute of Noise Control Engineering of Japan and the Acoustical Society of Japan. ; 4, s. 3453-3460
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Auralization of road-traffic noise may be a useful tool for city planning, for instance as a support to decisions regarding noise mitigation. However, to be useful, the auralizations need to be perceptually valid. That is, the auralized sounds should be perceptually indistinguishable from real sounds or, at least, similar with respect to perceptual factors crucial for correct decisions. For this reason, the auralization methodology developed in the Swedish LISTEN-project was perceptually evaluated. In four listening experiments, listeners assessed recordings and auralizations of the same car passages. Although real and auralized sounds were not completely indistinguishable, perfect discrimination was not possible. Moreover, good agreements between auralized and real sounds were found for perceived similarity, perceived annoyance and perceived speed. The results illustrate the usefulness of psychoacoustic methods and multivariate statistics for perceptual evaluation of auralizations and provide support for the validity of the LISTEN-approach to auralization.
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