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Transcriptional pro...
Transcriptional profiling of human embryonic stem cells differentiating to definitive and primitive endoderm and further toward the hepatic lineage.
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- Synnergren, Jane, 1967 (författare)
- Högskolan i Skövde,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Institutionen för vård och natur,Forskningscentrum för Systembiologi
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- Heins, Nico (författare)
- Cellartis AB, Göteborg
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- Brolén, Gabriella (författare)
- Cellartis AB, Göteborg
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- Eriksson, Gustav (författare)
- Cellartis AB, Göteborg
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- Lindahl, Anders, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Sahlgrenska University Hospital
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- Hyllner, Johan (författare)
- Cellartis AB, Göteborg
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- Olsson, Björn (författare)
- Högskolan i Skövde,Institutionen för vård och natur,Forskningscentrum för Systembiologi
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- Sartipy, Peter (författare)
- Cellartis AB, Göteborg
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- Björquist, Petter (författare)
- Cellartis AB, Göteborg
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2010
- 2010
- Engelska.
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Ingår i: Stem cells and development. - : Mary Ann Liebert Inc. - 1557-8534 .- 1547-3287. ; 19:7, s. 961-78
- Relaterad länk:
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http://his.diva-port...
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https://his.diva-por... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Human embryonic stem cells (hESC) can differentiate into a variety of specialized cell types, and they constitute a useful model system to study embryonic development in vitro. In order to fully utilize the potential of these cells, the mechanisms that regulate the developmental processes of specific lineage differentiation need to be better defined. The aim of this study was to explore the molecular program involved in the differentiation of hESC toward definitive endoderm (DE) and further into the hepatic lineage, and to compare that with primitive endoderm (PrE) differentiation. To that end, we applied two protocols: a specific DE differentiation protocol and an intrinsic differentiation protocol that mainly mediates PrE formation. We collected hESC, hESC-derived DE, DE-derived hepatocyte-progenitors (DE-Prog), DE-derived hepatocyte-like cells (DE-Hep), and the corresponding PrE derivatives. The samples were analyzed using microarrays, and we identified sets of genes that were exclusively up-regulated in DE derivatives (compared to PrE derivatives) at discrete developmental stages. We also investigated known protein interactions among the set of up-regulated genes in DE-Hep. The results demonstrate important differences between DE and PrE differentiation on the transcriptional level. In particular, our results identify a unique molecular program, exclusively activated during development of DE and the subsequent differentiation of DE toward the hepatic lineage. We identified key genes and pathways of potential importance for future efforts to improve hepatic differentiation from hESC. These results reveal new opportunities for rational design of specific interventions with the purpose of generating enriched populations of DE derivatives, including functional hepatocytes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Animals
- Cell Differentiation
- physiology
- Cell Lineage
- genetics
- Cells
- Cultured
- Embryonic Stem Cells
- cytology
- physiology
- Endoderm
- cytology
- physiology
- Gene Expression Profiling
- Gene Expression Regulation
- Developmental
- Genetic Markers
- Hepatocytes
- cytology
- physiology
- Humans
- Oligonucleotide Array Sequence Analysis
- Transcription
- Genetic
- Up-Regulation
- Naturvetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Synnergren, Jane ...
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Heins, Nico
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Brolén, Gabriell ...
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Eriksson, Gustav
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Lindahl, Anders, ...
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Hyllner, Johan
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visa fler...
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Olsson, Björn
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Sartipy, Peter
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Björquist, Pette ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Stem cells and d ...
- Av lärosätet
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Göteborgs universitet
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Högskolan i Skövde