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- Fang, Wenwen, et al.
(författare)
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Elastic and pH responsive hybrid interfaces created with engineered resilin and nanocellulose
- 2017
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Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 18:6, s. 1866-1873
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Tidskriftsartikel (refereegranskat)abstract
- We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.
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| 2. |
- Nilebäck, Linnea, et al.
(författare)
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Interfacial Behavior of Recombinant Spider Silk Protein Parts Reveals Cues on the Silk Assembly Mechanism
- 2018
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Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 34:39, s. 11795-11805
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Tidskriftsartikel (refereegranskat)abstract
- The mechanism of silk assembly, and thus the cues for the extraordinary properties of silk, can be explored by studying the simplest protein parts needed for the formation of silk-like materials. The recombinant spider silk protein 4RepCT, consisting of four repeats of polyalanine and glycine-rich segments (4Rep) and a globular C-terminal domain (CT), has previously been shown to assemble into silk-like fibers at the liquid-air interface. Herein, we study the interfacial behavior of the two parts of 4RepCT, revealing new details on how each protein part is crucial for the silk assembly. Interfacial rheology and quartz crystal microbalance with dissipation show that 4Rep interacts readily at the interfaces. However, organized nanofibrillar structures are formed only when 4Rep is fused to CT. A strong interplay between the parts to direct the assembly is demonstrated. The presence of either a liquid-air or a liquid-solid interface had a surprisingly similar influence on the assembly.
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| 3. |
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| 4. |
- Ruokolainen, Olli, et al.
(författare)
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Construct validity and reliability of Finnish version of Orebro Musculoskeletal Pain Screening Questionnaire
- 2016
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Ingår i: Scandinavian Journal of Pain. - : Elsevier. - 1877-8860 .- 1877-8879. ; 13, s. 148-153
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Chronic pain causes suffering for affected individuals and incurs costs to society through work disability. Interventions based on early screening of psychological risk factors for chronic pain using screening tools such as the Orebro Musculoskeletal Pain Screening Questionnaire (OMPSQ) have been found to reduce work absenteeism and health care visits and increase perceived health. The aim of the current study was to translate the OMPSQ into Finnish and test its validity and reliability in a patient sample.Methods: The OMPSQ was forward-backward translated and cross-culturally adapted, and applied to our study population (n = 69), the members of which had been referred to the Department of Physical and Rehabilitation Medicine of Oulu University Hospital from primary health care centres in Northern Finland due to chronic low back pain. The patients answered the OMPSQ two weeks before the hospital visit, and the follow-up questionnaire either during the hospital visit, or after by mail. The reliability of the OMPSQ was evaluated using intraclass correlation coefficients (ICC). Factor analysis was used to group items of the OMPSQ, and internal consistency between the items was determined by calculating Cronbach's alphas.Results: The cross-cultural adaptation revealed only minor semantic and cultural differences. Measurements showed reliability as moderate to nearly perfect for all of the OMPSQ items (ICC values ranged from 0.59 to 0.96). Items loaded into five different factors: disability, psychological symptoms, pain, fear avoidance, and work. All except one (work) showed acceptable internal consistency. The OMPSQ score was positively associated with both intensity of pain and the Oswestry Disability Index.Conclusions and implications: The reliability and construct validity of the Finnish version of the OMPSQ were good. The predictive ability of the OMPSQ in the Finnish population should be evaluated in further studies. (C) 2016 Scandinavian Association for the Study of Pain.
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