| 1. |
- Cantatore, Valentina, 1986, et al.
(författare)
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Design strategy of a graphene based bio-sensor for glucose
- 2018
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Ingår i: Carbon. - : Elsevier BV. - 0008-6223. ; 137, s. 343-348
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Tidskriftsartikel (refereegranskat)abstract
- A novel graphene-based glucose sensor-design is formulated and explored in silico. An ad hoc host molecule is tailored to bind to glucose by multiple hydrogen bonds. A pyridinic core is chosen for this receptor in order to allow for “socket-plug” dative bonding to boron sites of boron doped graphene. The modeling employs DFT (Density Functional Theory) together with an effective aqueous environment to take into account the solvation effect. High selectivity is demonstrated for the suggested host molecule towards glucose as compared to other possible competitors in blood such as fructose, biotin and ascorbic acid. A route to achieve improved sensitivity, exploiting the hydrophilic/hydrophobic properties of the host + glucose system for enhanced selective binding to the hydrophobic boron doped graphene support is discussed.
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| 2. |
- Wang, Nan, 1988, et al.
(författare)
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Efficient surface modification of carbon nanotubes for fabricating high performance CNT based hybrid nanostructures
- 2017
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Ingår i: Carbon. - : Elsevier BV. - 0008-6223. ; 111, s. 402-410
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Tidskriftsartikel (refereegranskat)abstract
- Carbon nanotubes (CNTs) were chemically modified to achieve strong binding strength with the attached functional components as well as good dispersability and nanoparticle size-uniformity. An efficient multi-oxidation process was developed to create porous out layer with many nanoscale defects on the surface of CNTs for metallic nanoparticle close attachment and bond sufficient oxygen-containing groups, which assisted the dispersion of CNTs in the aqueous solution. The surface modified CNTs have advantages of strong binding capability, large surface area, high mechanical strength and good dispersability, which show great potential as building blocks for hybrid nanomaterials. Monodispersed silver nano particles with an average size of 3 nm were formed from inside the created nanoscale defects on the surface of CNTs without any obvious agglomerations. The fabricated hybrid exhibited much enhanced anti-bacterial performance compared to commercial silver nanoparticles due to the combined antibacterial effects of CNTs and silver nanoparticles. With these superior properties, the developed surface modification process could be widely used for improving the performances of many CNT based hybrid nanomaterials in diverse applications.
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| 3. |
- Acet, Ömür, et al.
(författare)
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Inhibition of bacterial adhesion by epigallocatechin gallate attached polymeric membranes
- 2023
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Ingår i: Colloids and Surfaces B: Biointerfaces. - : Elsevier BV. - 0927-7765 .- 1873-4367. ; 221
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Tidskriftsartikel (refereegranskat)abstract
- Microbial adhesion and formation of biofilms cause a serious problem in several areas including but not limited to food spoilage, industrial corrosion and nosocomial infections. These microbial biofilms pose a serious threat to human health since microbial communities in the biofilm matrix are protected with exopolymeric substances and difficult to eradicate with antibiotics. Hence, the prevention of microbial adhesion followed by biofilm formation is one of the promising strategies to prevent these consequences. The attachment of antimicrobial agents, coatings of nanomaterials and synthesis of hybrid materials are widely used approach to develop surfaces having potential to hinder bacterial adhesion and biofilm formation. In this study, epigallocatechin gallate (EGCG) is attached on p(HEMA-co-GMA) membranes to prevent the bacterial colonization. The attachment of EGCG to membranes was proved by Fourier-transform infrared spectroscopy (FT-IR). The synthesized membrane showed porous structure (SEM), and desirable swelling degree, which are ideal when it comes to the application in biotechnology and biomedicine. Furthermore, EGCG attached membrane showed significant potential to prevent the microbial colonization on the surface. The obtained results suggest that EGCG attached polymer could be used as an alternative approach to prevent the microbial colonization on the biomedical surfaces, food processing equipment as well as development of microbial resistant food packaging systems.
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| 4. |
- Avaz, S., et al.
(författare)
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Graphene based nanosensor for aqueous phase detection of nitroaromatics
- 2017
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Ingår i: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 7:41, s. 25519-25527
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Tidskriftsartikel (refereegranskat)abstract
- A graphene-based nanosensor was fabricated to selectively detect nitrotriazolone (NTO) molecules with a molecularly imprinted film via simple electrical measurements. Molecularly imprinted polymer comprising chitosan was used as sensitive layer. Gold electrodes for electrical measurements were lithographically fabricated on Si/SiO2 substrate, followed by monolayer graphene transfer and polymeric film coating. Monolayer graphene and molecularly imprinted polymer were characterized by ATR-FTIR, UV-Vis, SEM and Raman spectroscopy. Transfer-length measurements (TLM) indicate that the sensor selectively and linearly responds against aqueous NTO solutions within a wide range of concentration of 0.01-0.1 mg mL(-1) that covers the lowest toxic level of NTO determined by USEPA. This nanosensor with embedded electrodes is re-usable and suitable for field applications, offering real-time electrical measurements unlike current techniques where complex analytics are required.
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| 5. |
- Cao, Zhejian, 1991, et al.
(författare)
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Synthesis of Metal-Organic Frameworks through Enzymatically Recycled Polyethylene Terephthalate
- 2023
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Ingår i: ACS Sustainable Chemistry & Engineering. - 2168-0485. ; 11:43, s. 15506-15512
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Tidskriftsartikel (refereegranskat)abstract
- Polyethylene terephthalate (PET) as one of the most produced plastics contributes to global waste pollution. Upcycling PET into value-added products therefore is of environmental and economic interest. Terephthalic acid (TPA), the monomer of PET, is a common linker for metal-organic framework (MOF) synthesis; thus, PET-to-MOF upcycling raises much research attention. However, conventional PET-to-MOF upcycling often requires PET depolymerization with strong acids or bases and high temperatures, which can lead to environmental and energy penalties. As an alternative, PETase offers a sustainable approach to depolymerizing PET under mesophilic and mild pH conditions. Here we report UiO-66, MOF-5, and MIL-101 syntheses using enzymatically recycled TPA as linkers. The enzymatically recycled TPA demonstrated low impurity, and the obtained MOFs possessed comparable crystallinity, thermal stability, and surface area. These results reveal the feasibility of MOF synthesis by using enzymatically recycled PET.
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| 6. |
- Chen, Xin, 1980, et al.
(författare)
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Graphene Oxide Attenuates Toxicity of Amyloid-β Aggregates in Yeast by Promoting Disassembly and Boosting Cellular Stress Response
- 2023
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Ingår i: Advanced Functional Materials. - 1616-3028 .- 1616-301X. ; 33:45
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Tidskriftsartikel (refereegranskat)abstract
- Alzheimer's disease (AD) is the most prevalent neurodegenerative disease, with the aggregation of misfolded amyloid-β (Aβ) peptides in the brain being one of its histopathological hallmarks. Recently, graphene oxide (GO) nanoflakes have attracted significant attention in biomedical areas due to their capacity of suppressing Aβ aggregation in vitro. The mechanism of this beneficial effect has not been fully understood in vivo. Herein, the impact of GO on intracellular Aβ42 aggregates and cytotoxicity is investigated using yeast Saccharomyces cerevisiae as the model organism. This study finds that GO nanoflakes can effectively penetrate yeast cells and reduce Aβ42 toxicity. Combination of proteomics data and follow-up experiments show that GO treatment alters cellular metabolism to increases cellular resistance to misfolded protein stress and oxidative stress, and reduces amounts of intracellular Aβ42 oligomers. Additionally, GO treatment also reduces HTT103QP toxicity in the Huntington's disease (HD) yeast model. The findings offer insights for rationally designing GO nanoflakes-based therapies for attenuating cytotoxicity of Aβ42, and potentially of other misfolded proteins involved in neurodegenerative pathology.
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| 7. |
- Chen, Yanyan, 1990, et al.
(författare)
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“Graphene nanospikes exert bactericidal effect through mechanical damage and oxidative stress”
- 2024
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Ingår i: Carbon. - 0008-6223. ; 218
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Tidskriftsartikel (refereegranskat)abstract
- Microbial contamination of biomedical surfaces is an important clinical challenge, driving the development of new antibacterial materials. Nanoprotrusions on the wing surface of some insects have intrinsic antibacterial and antifouling properties, which inspires fabrication of biomimetic nanopatterns on medical devices. Herein, we report a broad-spectrum bactericidal surface consisting of graphene nanospikes synthesized by plasma-enhanced chemical vapor deposition. Similar coatings have been reported before, but the killing mechanism and main parameters for efficiency of such coatings have not been clarified. We investigated the correlation of anti-biofilm efficiency of graphene nanospikes to their major physicochemical parameters. While height and thickness of nanospikes did not directly correlate with bactericidal effects, edge/defect density showed linear correlation with lethality for both Gram-negative and Gram-positive bacteria. We further demonstrated that the killing mechanism is synergistic, depending on physical rupture of bacterial membranes as well as considerable oxidative damage to the cells. Of note, for the first time, we quantify the level of oxidative stress induced by graphene nanospikes in two bacterial species using genetically encoded biosensors. Our work provides a fundamental understanding of the impact of various parameters of graphene nanostructures on the bactericidal efficiency, enabling rational design of graphene-based bactericidal surfaces.
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| 8. |
- Chen, Yanyan, 1990, et al.
(författare)
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Interactions Between Graphene-Based Materials and Biological Surfaces: A Review of Underlying Molecular Mechanisms
- 2021
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Ingår i: Advanced Materials Interfaces. - : Wiley. - 2196-7350. ; 8:24
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Forskningsöversikt (refereegranskat)abstract
- Understanding the underlying molecular mechanism of how graphene materials (GMs) interact with biological surfaces is the key to develop safe and effective biomedical applications of GMs. Here, a systematic and comprehensive mechanistic perspective of interactions between pristine GMs and biological membranes is provided. To this end, first the known mechanisms of interaction between GMs and membrane components are summarized and classified, with a focus on phospholipids, cholesterol, and membrane proteins. Both experimental observations and computational simulations are included. Detailed experimental conditions and physiochemical properties of GMs are listed for each cited application. At the end of this review, current challenges and conflicts that limit biomedical applications of GMs are discussed. Based on reported mechanisms, guidelines for future studies to address the remaining challenges are proposed, specifically with respect to modulating the intrinsic properties of GMs for more efficient and safer therapeutic applications.
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| 9. |
- Eswaran, Muthusankar, 1988, et al.
(författare)
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A flexible multifunctional electrode based on conducting PANI/Pd composite for non-enzymatic glucose sensor and direct alcohol fuel cell applications
- 2023
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Ingår i: Fuel. - : Elsevier BV. - 0016-2361. ; 345
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Tidskriftsartikel (refereegranskat)abstract
- In this work, we fabricated a flexible, multifunctional polyimide (PI)/Au-polyaniline (PAN)/Pd nanocomposite electrode with excellent electrochemical properties. Structural geometry, morphological views, and functional group analyses indicated that the physicochemical and electrochemical performance of the electrode is based on the strong and synergistic metal-polymer interaction between the conducting PAN and Pd, which ensured high conductivity, rapid response, and high electron transfer rate through more electroactive spots available in the nanocomposite. Here, we demonstrated that the fabricated PI/Au-PAN/Pd electrodes can be successfully used for biomedical sensing of glucose, as well as for energy conversion application, using the oxidation of alcohols such as methanol and ethanol in fuel cells. The electrochemical analysis shows that the flexible sensor (PI/Au-PAN/Pd) has ultra-high sensitivity of 2140 μA/μM.cm2 with a low detection limit of 0.3 μM for glucose. Also, the interference analysis, reproducibility, and stability studies reveal its excellent capability for glucose sensing. Furthermore, the electrode also demonstrates prominent electrocatalytic behavior to the electrooxidation of methanol and ethanol in an alkaline medium with a current density of 3 mA/cm2 and 0.96 mA/cm2 along with good cyclic stability. Thus, this efficient flexible electrocatalyst with good stability, practicability, and reproducibility claims its potential applications in flexible/wearable healthcare diagnostics systems as well as in alternative energy conversion devices.
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| 10. |
- Eswaran, Muthusankar, 1988, et al.
(författare)
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A Road Map toward Field-Effect Transistor Biosensor Technology for Early Stage Cancer Detection
- 2022
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Ingår i: Small Methods. - : Wiley. - 2366-9608.
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Forskningsöversikt (refereegranskat)abstract
- Field effect transistor (FET)-based nanoelectronic biosensor devices provide a viable route for specific and sensitive detection of cancer biomarkers, which can be used for early stage cancer detection, monitoring the progress of the disease, and evaluating the effectiveness of therapies. On the road to implementation of FET-based devices in cancer diagnostics, several key issues need to be addressed: sensitivity, selectivity, operational conditions, anti-interference, reusability, reproducibility, disposability, large-scale production, and economic viability. To address these well-known issues, significant research efforts have been made recently. An overview of these efforts is provided here, highlighting the approaches and strategies presently engaged at each developmental stage, from the design and fabrication of devices to performance evaluation and data analysis. Specifically, this review discusses the multistep fabrication of FETs, choice of bioreceptors for relevant biomarkers, operational conditions, measurement configuration, and outlines strategies to improve the sensing performance and reach the level required for clinical applications. Finally, this review outlines the expected progress to the future generation of FET-based diagnostic devices and discusses their potential for detection of cancer biomarkers as well as biomarkers of other noncommunicable and communicable diseases.
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