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- Santangelo, James S., et al.
(author)
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Global urban environmental change drives adaptation in white clover
- 2022
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 375
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Journal article (peer-reviewed)abstract
- Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural dines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale.
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| 2. |
- Garcia, Yedra, et al.
(author)
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Floral scent divergence across an elevational hybrid zone with varying pollinators
- 2023
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In: Oecologia. - : Springer Nature. - 0029-8549 .- 1432-1939. ; 201:1, s. 45-57
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Journal article (peer-reviewed)abstract
- Divergence in floral traits attractive to different pollinators can promote reproductive isolation in related species. When isolation is incomplete, hybridization may occur, which offers the opportunity to explore mechanisms underlying reproductive isolation. Recent work suggests that divergence in floral scent may frequently contribute to reproductive barriers, although such divergence has seldom been examined in species with generalized pollination. Here, we used two closely related Penstemon species, P. newberryi and P. davidsonii, and their natural hybrids from an elevational gradient with pollinator communities that are predicted to vary in their reliance on floral scent (i.e., primarily hummingbirds at low elevation vs. bees at high elevation). The species vary in a suite of floral traits, but scent is uncharacterized. To address whether scent varies along elevation and potentially contributes to reproductive isolation, we genetically characterized individuals collected at field and identified whether they were parental species or hybrids. We then characterized scent amount and composition. Although the parental species had similar total emissions, some scent characteristics (i.e., scent composition, aromatic emission) diverged between them and may contribute to their isolation. However, the species emitted similar compound sets which could explain hybridization in the contact area. Hybrids were similar to the parents for most scent traits, suggesting that their floral scent would not provide a strong barrier to backcrossing. Our study suggests floral scent may be a trait contributing to species boundaries even in plants with generalized pollination, and reinforces the idea that evolutionary pollinator transitions may involve changes in multiple floral traits.
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| 7. |
- Burdon, Rosalie C. F., et al.
(author)
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Spatiotemporal floral scent variation of Penstemon digitalis
- 2015
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In: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 41:7, s. 641-650
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Journal article (peer-reviewed)abstract
- Variability in floral volatile emissions can occur temporally through floral development, during diel cycles, as well as spatially within a flower. These spatiotemporal patterns are hypothesized to provide additional information to floral visitors, but they are rarely measured, and their attendant hypotheses are even more rarely tested. In Penstemon digitalis, a plant whose floral scent has been shown to be under strong phenotypic selection for seed fitness, we investigated spatiotemporal variation in floral scent by using dynamic headspace collection, respectively solid-phase microextraction, and analyzed the volatile samples by combined gas chromatography-mass spectrometry. Total volatile emission was greatest during flowering and peak pollinator activity hours, suggesting its importance in mediating ecological interactions. We also detected tissue and reward-specific compounds, consistent with the hypothesis that complexity in floral scent composition reflects several ecological functions. In particular, we found tissue-specific scents for the stigma, stamens, and staminode (a modified sterile stamen common to all Penstemons). Our findings emphasize the dynamic nature of floral scents and highlight a need for greater understanding of ecological and physiological mechanisms driving spatiotemporal patterns in scent production.
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| 8. |
- Burdon, Rosalie, 1988-, et al.
(author)
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Scented nectar and the challenge of measuring honest signals in pollination
- 2020
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In: Journal of Ecology. - : WILEY. - 0022-0477 .- 1365-2745. ; 108:5, s. 2132-2144
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Journal article (peer-reviewed)abstract
- Nectar scents are thought to function as honest signals of reward used by pollinators, but this hypothesis has rarely been tested. UsingPenstemon digitalis, we examined honest signalling of the nectar volatile (S)-(+)-linalool and pollinator responses to linalool in both field and laboratory settings. Because our previous work showed that linalool emission was associated with higher female fitness and that nectar is scented with linalool, we hypothesized that linalool was an honest signal of nectar reward. To assess honesty, we measured linalool-nectar associations including nectar volume, sugar amount, concentration and production rate for inflorescences and flowers in several populations. We also assessed whetherBombus impatiens, the main pollinator ofP. digitalisat our sites, can use linalool as a foraging signal. We supplemented real or artificial flowers in the field and laboratory with varying linalool-nectar combinations to measure pollinator behavioural responses. We found that an inflorescence's linalool emissions could be used to predict nectar rewards inP. digitalis, but this was driven by indirect associations with display size rather than directly advertising more profitable flowers. For flowers within inflorescences there was also no evidence for an association between signal and reward. Field tests of bumblebee behaviour were inconclusive. However, in laboratory assays, bumblebees generally used variation in linalool emissions to choose more profitable flowers, demonstrating they can detect differences in linalool emitted byP. digitalisand associate them with reward profitability. These results suggest experiments that decouple display size, scent and reward are necessary to assess whether (and when) bees prefer higher linalool emissions. Bees preferred nectars with lower linalool concentrations when linalool flavoured the nectar solution, suggesting the potential for conflicting pressures on scent emission in the field. Synthesis. Our results highlight the challenges of assessing function for traits important to fitness and suggest that the perception of floral signalling honesty may depend on whether pollinators use inflorescences or flowers within inflorescences when making foraging decisions. We conclude that future research on honest signalling in flowering plants, as well as its connection to phenotypic selection, should explicitly define honesty, in theoretical and experimental contexts.
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| 9. |
- Burdon, Rosalie, 1988-
(author)
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The Ecology of Floral Signals in Penstemon digitalis
- 2016
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Doctoral thesis (other academic/artistic)abstract
- In this thesis, I combined field observations and lab experiments to explore the ecological significance of floral signals in a North American wildflower, Penstemon digitalis. More specifically, to determine the potential mechanisms driving selection on floral scent, I studied how scent mediates interactions with pollinators and antagonists by (1) observing spatiotemporal variation in scent emission (2), floral volatile ability to suppress microbes (3) the honest advertisement of nectar, and (4) if scent could aid pollinator learning by reinforcing visual signals.Scent sampling of flower development, flower tissues, rewards and inflorescence day/night emission, revealed a complexity in floral scent composition and emission that could reflect several ecological functions. The floral bouquet of P. digitalis was strongest when flowers opened, primarily emitted from flower nectaries and was strongest during the day when pollinators are most active, suggesting a role in plant-pollinator interactions.Because linalool was one of the few floral compounds found in nectar where microbe growth can degrade the pollinator reward, I studied its role in plant-microbe interactions. Bacteria strains isolated from floral and vegetative tissues were exposed to varying concentrations of nectar volatiles: linalool and methyl nicotinate. Linalool inhibited bacteria growth rate from all tissue origins whereas methyl nicotinate had little effect, suggesting that microbes could drive selection on linalool emission strength. To determine the extent that linalool could honestly signal nectar availability, linalool-nectar associations were measured for inflorescences and flowers. Linalool predicted inflorescence nectar availability but not flower, exposing a limit to its honesty. Pollinator Bombus impatiens could use linalool as a foraging signal at varying concentrations, suggesting linalool could be learned and used to choose the most rewarding plants. Measurement and comparison of signal-reward associations for both olfactory and visual signals/cues of P. digitalis displays found display size and linalool honest indicators of nectar. Lab behaviour experiments showed multiple signals correlated with reward could increase bumblebee foraging efficiency and promote learning, providing an explanation for why floral displays are complex and consist of multiple signals. Together my results show that an integrated approach is required to understand the mechanisms driving the evolution of the floral phenotype.
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| 10. |
- Caruso, Christina M., et al.
(author)
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Do Plants Eavesdrop on Floral Scent Signals?
- 2016
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In: Trends in Plant Science. - : Elsevier BV. - 1360-1385 .- 1878-4372. ; 21:1, s. 9-15
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Research review (peer-reviewed)abstract
- Plants emit a diverse array of volatile organic compounds that can function as cues to other plants. Plants can use volatiles emitted by neighbors to gain information about their environment, and respond by adjusting their phenotype. Less is known about whether the many different volatile signals that plants emit are all equally likely to function as cues to other plants. We review evidence for the function of floral volatile signals and conclude that plants are as likely to perceive and respond to floral volatiles as to other, better-studied volatiles. We propose that eavesdropping on floral volatile cues is particularly likely to be adaptive because plants can respond to these cues by adjusting traits that directly affect pollination and mating.
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