| 1. |
- Johansson, Henrik J., et al.
(författare)
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Breast cancer quantitative proteome and proteogenomic landscape
- 2019
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Ingår i: Nature Communications. - : NATURE PUBLISHING GROUP. - 2041-1723. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- In the preceding decades, molecular characterization has revolutionized breast cancer (BC) research and therapeutic approaches. Presented herein, an unbiased analysis of breast tumor proteomes, inclusive of 9995 proteins quantified across all tumors, for the first time recapitulates BC subtypes. Additionally, poor-prognosis basal-like and luminal B tumors are further subdivided by immune component infiltration, suggesting the current classification is incomplete. Proteome-based networks distinguish functional protein modules for breast tumor groups, with co-expression of EGFR and MET marking ductal carcinoma in situ regions of normal-like tumors and lending to a more accurate classification of this poorly defined subtype. Genes included within prognostic mRNA panels have significantly higher than average mRNA-protein correlations, and gene copy number alterations are dampened at the protein-level; underscoring the value of proteome quantification for prognostication and phenotypic classification. Furthermore, protein products mapping to non-coding genomic regions are identified; highlighting a potential new class of tumor-specific immunotherapeutic targets.
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| 2. |
- Tekpli, Xavier, et al.
(författare)
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An independent poor-prognosis subtype of breast cancer defined by a distinct tumor immune microenvironment
- 2019
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- How mixtures of immune cells associate with cancer cell phenotype and affect pathogenesis is still unclear. In 15 breast cancer gene expression datasets, we invariably identify three clusters of patients with gradual levels of immune infiltration. The intermediate immune infiltration cluster (Cluster B) is associated with a worse prognosis independently of known clinicopathological features. Furthermore, immune clusters are associated with response to neoadjuvant chemotherapy. In silico dissection of the immune contexture of the clusters identified Cluster A as immune cold, Cluster C as immune hot while Cluster B has a pro-tumorigenic immune infiltration. Through phenotypical analysis, we find epithelial mesenchymal transition and proliferation associated with the immune clusters and mutually exclusive in breast cancers. Here, we describe immune clusters which improve the prognostic accuracy of immune contexture in breast cancer. Our discovery of a novel independent prognostic factor in breast cancer highlights a correlation between tumor phenotype and immune contexture.
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| 3. |
- Zyoud, Ahed, et al.
(författare)
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CdS/FTO thin film electrodes deposited by chemical bath deposition and by electrochemical deposition : A comparative assessment of photo-electrochemical characteristics
- 2013
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Ingår i: Solid State Sciences. - : Elsevier. - 1293-2558 .- 1873-3085. ; 18, s. 83-90
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Tidskriftsartikel (refereegranskat)abstract
- CdS thin films have been deposited onto FTO/glass substrates by two different techniques, electro-chemical deposition (ECD) and chemical bath deposition (CBD). Feasibility of using these two film types in photoelectrochemical processes has been critically investigated here. The films were comparatively characterized by a number of techniques (solid state absorption spectra, solid state photoluminescence spectra, XRD and SEM). PEC characteristics of the electrodes, including current density voltage (J-V) plots, conversion efficiency (eta), stability and fill-factor (FF) were then studied. The results show that both systems involved nano-sized CdS particles living in coagulates. The ECD was thinner and more uniform than the CBD system. The CBD films were more effective in PEC processes than the ECD counterparts. Effect of annealing on characteristics of both electrode systems has been investigated. Annealing enhanced both film characteristics, but the CBD was affected to a higher extent, and the annealed CBD film was more effective than the ECD counterpart. (C) 2013 Elsevier Masson SAS. All rights reserved.
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| 4. |
- Zyoud, Ahed, et al.
(författare)
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Combined electrochemical/chemical bath depositions to prepare CdS film electrodes with enhanced PEC characteristics
- 2013
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Ingår i: Journal of Electroanalytical Chemistry. - : Elsevier BV. - 0022-0728 .- 1873-2569 .- 1572-6657. ; 707, s. 117-121
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Tidskriftsartikel (refereegranskat)abstract
- A new method to prepare CdS film electrodes, based on electrochemical deposition (ECD), followed by chemical bath deposition (CBD), is described. The ECD/CBD-CdS film electrodes combine the advantages of both ECD-CdS film (good adherence to FTO/glass substrate) and CBD-CdS film (suitable film thickness) together. The new ECD/CBD electrode showed higher photo-electrochemical (PEC) efficiency and stability than either ECD- or CBD-CdS film electrodes, especially after annealing. Inter-particle connection and uniformity of the ECD/CBD-CdS film were further enhanced by annealing. Consequently, the electrode PEC conversion efficiency and stability were enhanced. The combined preparation technique, followed by annealing, is potentially useful for future manufacturing of CdS and other film electrode systems. (C) 2013 Elsevier B.V. All rights reserved.
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