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Sökning: WFRF:(Paulo Paula) > Malmö universitet

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1.
  • Moura, Camilla G, et al. (författare)
  • Evaluation of osteogenic cell culture and osteogenic/peripheral blood mononuclear human cell co-culture on modified titanium surfaces
  • 2013
  • Ingår i: Biomedical Materials. - : Bibliopolis, Edizioni di Filosofia e Scienze. - 1748-6041 .- 1748-605X. ; 8:3
  • Tidskriftsartikel (refereegranskat)abstract
    • This study aimed to determine the effect of a bioactive ceramic coating on titanium in the nanothickness range on human osteogenic cells, peripheral blood mononuclear cells (PBMC) and on osteogenic cells co-cultured with PBMC without exogenous stimuli. Cell viability, proliferation, adhesion, cytokine release (IL1β, TGFβ1, IL10 and IL17) and intracellular stain for osteopontin and alkaline phosphatase were assessed. Morphologic evaluation showed smaller and less spread cell aspects in co-culture relative to osteogenic cell culture. Cell viability, proliferation and adhesion kinetics were differently influenced by surface texture/chemistry in culture versus co-culture. Cytokine release was also influenced by the interaction between mononuclear and osteogenic cells (mediators released by mononuclear cells acted on osteogenic cells and vice versa). In general, 'multi-cell type' interactions played a more remarkable role than the surface roughness or chemistry utilized on the in vitro cellular events related to initial stages of bone formation.
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2.
  • Neiva, Rodrigo, et al. (författare)
  • Guidelines for Clinical Management of Laser-Etched (Laser-Lok) Abutments in Two Different Clinical Scenarios : A Preclinical Laboratory Soft Tissue Assessment Study
  • 2016
  • Ingår i: The international journal of periodontics & restorative dentistry. - : Quintessence. - 0198-7569 .- 1945-3388. ; 36:3, s. 339-345
  • Tidskriftsartikel (refereegranskat)abstract
    • One-stage implants were placed in the mandibles of eight beagle dogs with laser-etched (LL) and machined abutments. After 4 weeks, half of the LL abutments were disconnected and reconnected after 10 minutes of saline storage, and the other half were replaced with a new LL abutment (impression simulation) with or without sulcus de-epithelialization. After abutment change, systems remained in vivo for 3 weeks. Results showed that LL abutments can be reconnected and that sulcus scoring prior to LL placement of one-stage implants receiving machined abutments may be beneficial.
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