| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- de Azevedo, Adalberto Mantovani Martiniano, et al.
(författare)
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Inclusive Waste Governance and Grassroots Innovations for Social, Environmental And Economic Change
- 2018
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Participants of two research projects (Recycling Networks: Grassroots resilience tackling climate, environmental and poverty challenges (funded by the Swedish Research Council) and Mapping Waste Governance (funded by the Social Sciences and Humanities Research Council of Canada) collaborate in offering a critical inter- and transdisciplinary perspective on waste and waste actors (waste picker cooperatives, associations, community-based organizations, partnerships, networks and NGOs). The research is conducted in the following cities: Buenos Aires (Argentina), São Paulo (Brazil), Vancouver and Montreal (Canada), Kisumu (Kenya), Managua (Nicaragua) and Dar es Salaam (Tanzania). Together we examine the challenges that innovative grassroots initiatives and networks encounter in generating livelihoods to improve household waste collection and recycling, particularly in informal settlements of global South cities. We seek to map waste governance and successful waste management initiatives, arrangements and policies involving grassroots initiatives. In this report, we present a brief description of solid waste governance in the cities where we conducted fieldwork. We then illuminate some of our findings on grassroots innovations involving waste pickers or waste workers in these cities. Both research projects combine multi-case studies of waste picker groups and local government initiatives, apply qualitative research tools and participatory action research (e.g. photo voice, participant observation, workshops, surveys and interviews). We are interested in understanding processes, challenges and opportunities related to how these grassroots initiatives and networks operate to bring about socio-environmental and economic change? How they address challenges and what the assets are in everyday waste governance that can be explored to make waste governance more sustainable and thus more inclusive? Researchers involved in these two projects, key stakeholders from grassroots initiatives in these countries, representatives from some international waste picker networks and local and regional government officials from Kisumu, Kenya, met between 23rd and 29th of April 2018, in Kisumu to present and discuss the results of the first year of research activities, which are herewith documented.
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| 5. |
- Garcia-Martinez, Fernando, et al.
(författare)
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Structure Matters: Asymmetric CO Oxidation at Rh Steps with Different Atomic Packing
- 2022
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 144:33, s. 15363-15371
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Tidskriftsartikel (refereegranskat)abstract
- Curved crystals are a simple but powerful approach to bridge the gap between single crystal surfaces and nanoparticle catalysts, by allowing a rational assessment of the role of active step sites in gas-surface reactions. Using a curved Rh(111) crystal, here, we investigate the effect of A-type (square geometry) and B-type (triangular geometry) atomic packing of steps on the catalytic CO oxidation on Rh at millibar pressures. Imaging the crystal during reaction ignition with laser-induced CO2 fluorescence demonstrates a two-step process, where B-steps ignite at lower temperature than A-steps. Such fundamental dissimilarity is explained in ambient pressure X-ray photoemission (AP-XPS) experiments, which reveal partial CO desorption and oxygen buildup only at B-steps. AP-XPS also proves that A-B step asymmetries extend to the active stage: at A-steps, low-active O-Rh-O trilayers buildup immediately after ignition, while highly active chemisorbed O is the dominant species on B-type steps. We conclude that B-steps are more efficient than A-steps for the CO oxidation.
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| 6. |
- Hausmair, Katharina, 1982, et al.
(författare)
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Prediction of Nonlinear Distortion in Wideband Active Antenna Arrays
- 2017
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Ingår i: IEEE Transactions on Microwave Theory and Techniques. - 0018-9480 .- 1557-9670. ; 65:11, s. 4550-4563
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we propose a technique for comprehensive analysis of nonlinear and dynamic characteristics of multi-antenna transmitters (TXs). The analysis technique is enabled by the development of a Volterra series-based dual-input model for power amplifiers (PAs), which is capable of taking into account the joint effects of PA nonlinearity, antenna crosstalk and mismatch for wideband modulated signals. By combining multiple instances of the PA model with linear dynamic antenna simulations we develop the analysis technique. The proposed method allows the prediction of the output signal of every antenna in an arbitrarily sized TX array, as well as the total far-field radiated wave of the TX for any input signal with low computational effort. A 2.12 GHz four-element TX demonstrator based on GaAs PAs is implemented to verify simulation results with measurements. The proposed technique is a powerful tool to study hardware characteristics, as for example the effects of antenna design and element spacing. It can be used in cases where experiments are not feasible, and thus aid the development of next generation wireless systems.
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| 7. |
- Langer, Judith, et al.
(författare)
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Present and Future of Surface-Enhanced Raman Scattering
- 2020
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-086X .- 1936-0851. ; 14:1, s. 28-117
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Forskningsöversikt (refereegranskat)abstract
- The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article. ©
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| 8. |
- van Buggenhout, Sandra, et al.
(författare)
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Structural design of natural plant based foods to promote nutritional quality
- 2012
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Ingår i: Trends in Food Science and Technology. - : Elsevier BV. - 0924-2244 .- 1879-3053. ; 24:1, s. 47-59
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- During traditional industrial processing of fruit and vegetable derived foods, consideration of the eventual retention, bio-accessibility or bio-availability of nutrients has hitherto been a secondary priority. Indeed standard processing of soups and sauces involves treating all ingredients in a similar way, usually by prolonged heat treatment and results in both lowered nutritional value and sensory quality of food products. Such products are typically structured using various additives including starches, gums and stabilizers, which consumers regard as unnatural, rather than exploiting the endogenous structuring potential of the fruit and vegetable ingredients as available at the farm gate. Recent studies undertaken within the EU sponsored 'Healthy Structuring' project have shown how to design an industrially manufactured product with good sensory qualities that is natural and nutritious. The strategy has been to use the inherent structural and nutritional properties of the raw material ingredients, on the one hand to ensure the textural quality of the final product without the use of artificial gums or stabilizers and, on the other hand to optimize the nutritional quality of the final product. This review briefly summarizes both the approach taken and the results obtained within the project.
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| 9. |
- Zapata Campos, María José, 1972, et al.
(författare)
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Inclusive recycling movements: a green deep democracy from below
- 2020
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Ingår i: Environment and Urbanization. - : SAGE Publications. - 0956-2478 .- 1746-0301. ; In Press
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Tidskriftsartikel (refereegranskat)abstract
- This paper examines the multiple strategies articulated by grassroots recycler networks to bring about socioenvironmental change. The paper shows how these networks are an emblematic case of grassroots governmentality, whereby urban poor communities contribute to building more inclusive environmental regimes by developing technologies of power more typical of the powerful. These technologies include enumeration, with its resulting self-knowledge; the production of discourses and rationalities of social inclusion and environmental sustainability; and engagement in open and diverse alliances, at times with actors holding apparently antagonistic interests. The paper also reveals how recycling networks are a representative case of deep and green democracy. It is deep democracy, as grassroots networks strive to gain deep and true representativeness in their territories. It is green democracy, as it illustrates alternative pathways to environmental governance that is not limited to state and global organizations, but that also includes a range of control techniques emanating from the communities themselves.
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| 10. |
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