| 1. |
- Marto, João Pedro, et al.
(författare)
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Safety and Outcome of Revascularization Treatment in Patients With Acute Ischemic Stroke and COVID-19: The Global COVID-19 Stroke Registry.
- 2023
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Ingår i: Neurology. - 1526-632X. ; 100:7
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Tidskriftsartikel (refereegranskat)abstract
- COVID-19-related inflammation, endothelial dysfunction, and coagulopathy may increase the bleeding risk and lower the efficacy of revascularization treatments in patients with acute ischemic stroke (AIS). We aimed to evaluate the safety and outcomes of revascularization treatments in patients with AIS and COVID-19.This was a retrospective multicenter cohort study of consecutive patients with AIS receiving intravenous thrombolysis (IVT) and/or endovascular treatment (EVT) between March 2020 and June 2021 tested for severe acute respiratory syndrome coronavirus 2 infection. With a doubly robust model combining propensity score weighting and multivariate regression, we studied the association of COVID-19 with intracranial bleeding complications and clinical outcomes. Subgroup analyses were performed according to treatment groups (IVT-only and EVT).Of a total of 15,128 included patients from 105 centers, 853 (5.6%) were diagnosed with COVID-19; of those, 5,848 (38.7%) patients received IVT-only and 9,280 (61.3%) EVT (with or without IVT). Patients with COVID-19 had a higher rate of symptomatic intracerebral hemorrhage (SICH) (adjusted OR 1.53; 95% CI 1.16-2.01), symptomatic subarachnoid hemorrhage (SSAH) (OR 1.80; 95% CI 1.20-2.69), SICH and/or SSAH combined (OR 1.56; 95% CI 1.23-1.99), 24-hour mortality (OR 2.47; 95% CI 1.58-3.86), and 3-month mortality (OR 1.88; 95% CI 1.52-2.33). Patients with COVID-19 also had an unfavorable shift in the distribution of the modified Rankin score at 3 months (OR 1.42; 95% CI 1.26-1.60).Patients with AIS and COVID-19 showed higher rates of intracranial bleeding complications and worse clinical outcomes after revascularization treatments than contemporaneous non-COVID-19 patients receiving treatment. Current available data do not allow direct conclusions to be drawn on the effectiveness of revascularization treatments in patients with COVID-19 or to establish different treatment recommendations in this subgroup of patients with ischemic stroke. Our findings can be taken into consideration for treatment decisions, patient monitoring, and establishing prognosis.The study was registered under ClinicalTrials.gov identifier NCT04895462.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
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| 4. |
- Enander, J., et al.
(författare)
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Prevalence and heritability of body dysmorphic symptoms in adolescents and young adults: a population-based nationwide twin study
- 2018
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Ingår i: Psychological Medicine. - : Cambridge University Press (CUP). - 0033-2917 .- 1469-8978. ; 48:16, s. 2740-2747
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Tidskriftsartikel (refereegranskat)abstract
- Background. Body dysmorphic disorder (BDD) usually begins during adolescence but little is known about the prevalence, etiology, and patterns of comorbidity in this age group. We investigated the prevalence of BDD symptoms in adolescents and young adults. We also report on the relative importance of genetic and environmental influences on BDD symptoms, and the risk for co-existing psychopathology. Methods. Prevalence of BDD symptoms was determined by a validated cut-off on the Dysmorphic Concerns Questionnaire (DCQ) in three population-based twin cohorts at ages 15 (n = 6968), 18 (n = 3738), and 20-28 (n = 4671). Heritability analysis was performed using univariate model-fitting for the DCQ. The risk for co-existing psychopathology was expressed as odds ratios (OR). Results. The prevalence of clinically significant BDD symptoms was estimated to be between 1 and 2% in the different cohorts, with a significantly higher prevalence in females (1.3-3.3%) than in males (0.2-0.6%). The heritability of body dysmorphic concerns was estimated to be 49% (95% CI 38-54%) at age 15, 39% (95% CI 30-46) at age 18, and 37% (95% CI 29-42) at ages 20-28, with the remaining variance being due to non-shared environment. ORs for co-existing neuropsychiatric and alcohol-related problems ranged from 2.3 to 13.2. Conclusions. Clinically significant BDD symptoms are relatively common in adolescence and young adulthood, particularly in females. The low occurrence of BDD symptoms in adolescent boys may indicate sex differences in age of onset and/or etiological mechanisms. BDD symptoms are moderately heritable in young people and associated with an increased risk for co-existing neuropsychiatric and alcohol-related problems.
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| 5. |
- García-Martínez, José, et al.
(författare)
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The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons.
- 2016
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Ingår i: Nucleic acids research. - : Oxford University Press (OUP). - 1362-4962 .- 0305-1048. ; 44:8, s. 3643-3658
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Tidskriftsartikel (refereegranskat)abstract
- We analyzed 80 different genomic experiments, and found a positive correlation between both RNA polymerase II transcription and mRNA degradation with growth rates in yeast. Thus, in spite of the marked variation in mRNA turnover, the total mRNA concentration remained approximately constant. Some genes, however, regulated their mRNA concentration by uncoupling mRNA stability from the transcription rate. Ribosome-related genes modulated their transcription rates to increase mRNA levels under fast growth. In contrast, mitochondria-related and stress-induced genes lowered mRNA levels by reducing mRNA stability or the transcription rate, respectively. We also detected these regulations within the heterogeneity of a wild-type cell population growing in optimal conditions. The transcriptomic analysis of sorted microcolonies confirmed that the growth rate dictates alternative expression programs by modulating transcription and mRNA decay.The regulation of overall mRNA turnover keeps a constant ratio between mRNA decay and the dilution of [mRNA] caused by cellular growth. This regulation minimizes the indiscriminate transmission of mRNAs from mother to daughter cells, and favors the response capacity of the latter to physiological signals and environmental changes. We also conclude that, by uncoupling mRNA synthesis from decay, cells control the mRNA abundance of those gene regulons that characterize fast and slow growth.
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| 6. |
- Glaros, Vassilis, et al.
(författare)
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Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response
- 2021
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Ingår i: Immunity. - : Elsevier. - 1074-7613 .- 1097-4180. ; 54:9, s. 2005-
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Tidskriftsartikel (refereegranskat)abstract
- Cell fate decisions during early B cell activation determine the outcome of responses to pathogens and vaccines. We examined the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing. Early after immunization, a homogeneous population of activated precursors (APs) gave rise to a transient wave of plasmablasts (PBs), followed a day later by the emergence of germinal center B cells (GCBCs). Most APs rapidly exited the cell cycle, giving rise to non-GC-derived early memory B cells (eMBCs) that retained an AP-like transcriptional profile. Rapid decline of antigen availability controlled these events; provision of excess antigen precluded cell cycle exit and induced a new wave of PBs. Fate mapping revealed a prominent contribution of eMBCs to the MBC pool. Quiescent cells with an MBC phenotype dominated the early response to immunization in primates. A reservoir of APs/eMBCs may enable rapid readjustment of the immune response when failure to contain a threat is manifested by increased antigen availability.
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| 7. |
- Kain, Jaan-Henrik, 1960, et al.
(författare)
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Characteristics, challenges and innovations of waste picker organizations: A comparative perspective between Latin American and East African countries
- 2022
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 17:7
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Tidskriftsartikel (refereegranskat)abstract
- Waste picker organisations (WPOs) around the globe collect, transport and process waste to earn their living but represent a widely excluded, marginalised and impoverished segment of society. WPOs are highly innovative, created by grassroots out of “nothing” to deliver economic, social and environmental sustainability. Still, we do not know how such innovations are developed, and how they are disseminated and adopted by other groups. This article examines characteristics, challenges and innovations of WPOs across five countries in Latin America and East Africa. It is based on quantitative and qualitative data regarding modes of organisation and management, gender, received support, business orientations, environmental and social contributions, and innovations developed in response to multiple challenges. The paper provides a comprehensive understanding of WPOs’ activities and their grassroots innovations in the Global South. The study shows how WPOs contribute significantly to the economic, social and environmental sustainability of the societies they serve as well as the wider urban societies. To start and maintain WPOs in informal settlements with a lack of infrastructure, institutional frameworks, and public and private investors is a difficult quest. WPOs take many different organisational forms depending on the complexity of local realities, ranging from advanced collective organization as cooperatives to small self-help groups and microentrepreneurs. Self-organisation into regional and national networks provides economic opportunities, autonomy and stability as well as political influence. Yet, institutional support is fundamental and the lack thereof threatens their existence. Sustaining WPOs as important providers of socio-environmental benefits through governmental and non-governmental actions is a worthwhile undertaking that builds sustainability.
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| 8. |
- Perez-Alcazar, Marta, et al.
(författare)
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Human Cerebrospinal Fluid Promotes Neuronal Viability and Activity of Hippocampal Neuronal Circuits In Vitro
- 2016
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Ingår i: Frontiers in Cellular Neuroscience. - : Frontiers Media SA. - 1662-5102. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- For decades it has been hypothesized that molecules within the cerebrospinal fluid (CSF) diffuse into the brain parenchyma and influence the function of neurons. However, the functional consequences of CSF on neuronal circuits are largely unexplored and unknown. A major reason for this is the absence of appropriate neuronal in vitro model systems, and it is uncertain if neurons cultured in pure CSF survive and preserve electrophysiological functionality in vitro. In this article, we present an approach to address how human CSF (hCSF) influences neuronal circuits in vitro. We validate our approach by comparing the morphology, viability, and electrophysiological function of single neurons and at the network level in rat organotypic slice and primary neuronal cultures cultivated either in hCSF or in defined standard culture media. Our results demonstrate that rodent hippocampal slices and primary neurons cultured in hCSF maintain neuronal morphology and preserve synaptic transmission. Importantly, we show that hCSF increases neuronal viability and the number of electrophysiologically active neurons in comparison to the culture media. In summary, our data indicate that hCSF represents a physiological environment for neurons in vitro and a superior culture condition compared to the defined standard media. Moreover, this experimental approach paves the way to assess the functional consequences of CSF on neuronal circuits as well as suggesting a novel strategy for central nervous system (CNS) disease modeling.
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| 9. |
- Zapata Campos, María José, 1972, et al.
(författare)
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Grassroots innovations in 'extreme' urban environments. The inclusive recycling movement
- 2023
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Ingår i: Environment and Planning C: Politics and Space. - : SAGE Publications. - 2399-6544 .- 2399-6552. ; 41:2, s. 351-374
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Tidskriftsartikel (refereegranskat)abstract
- Waste pickers all over the world work innovatively to reduce the environmental footprint of cities as they struggle to meet their critical livelihood obligations. Informed by the case of waste picker organizations (WPOs) this article examines how grassroots initiatives and extreme-niche innovations are created and sustained by mobilizing resources, rationales and relations. The study is informed by a cross-national survey and in-depth interviews with WPOs in Argentina, Brazil, Nicaragua, Kenya and Tanzania, and builds upon theories of grassroots innovation movements. The findings show how operating in contexts of extreme scarcity, these grassroots organisations tap into local resources, e.g. tacit knowledge, economies of affection and other socially embedded institutional resources. Blending material and environmental rationales, contributes to expanding their audiences and to gaining further support. In such deprived urban contexts, radical and cumulative crises and events hindering residents’ livelihoods can paradoxically also spark ingenuity out of necessity, and the transformation of these settings into extreme niches of innovation. Finally, the mobilization of relations through the formation of networks linking WPOs with supportive intermediaries and global circuits of solidarity becomes another fundamental resilience strategy by which WPOs can navigate contested environments and insert their extreme-niche innovations in governmental structures. By simultaneously adopting a broad repertoire of strategies of insertion, contention, and mobilization WPO and their innovations thrive in highly constrained environments. We conclude with reflecting on how ‘extreme’ niches of innovation − at the cracks of the formal city, economy and waste systems − can unleash the creative power of stigmatized, illiterate and neglected grassroots to experiment with new solutions in resource-poor environments.
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| 10. |
- Zapata Campos, María José, 1972, et al.
(författare)
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Inclusive recycling movements: a green deep democracy from below
- 2020
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Ingår i: Environment and Urbanization. - : SAGE Publications. - 0956-2478 .- 1746-0301. ; In Press
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Tidskriftsartikel (refereegranskat)abstract
- This paper examines the multiple strategies articulated by grassroots recycler networks to bring about socioenvironmental change. The paper shows how these networks are an emblematic case of grassroots governmentality, whereby urban poor communities contribute to building more inclusive environmental regimes by developing technologies of power more typical of the powerful. These technologies include enumeration, with its resulting self-knowledge; the production of discourses and rationalities of social inclusion and environmental sustainability; and engagement in open and diverse alliances, at times with actors holding apparently antagonistic interests. The paper also reveals how recycling networks are a representative case of deep and green democracy. It is deep democracy, as grassroots networks strive to gain deep and true representativeness in their territories. It is green democracy, as it illustrates alternative pathways to environmental governance that is not limited to state and global organizations, but that also includes a range of control techniques emanating from the communities themselves.
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