| 1. |
- Agustin, Sanchez-Arcilla, et al.
(författare)
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Introduction
- 2008
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Ingår i: Journal of Hydraulic Research. - 0022-1686. ; 46:2, s. 179-182
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Bidartondo, Martin, et al.
(författare)
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Preserving accuracy in GenBank
- 2008
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Ingår i: Science. ; 319:5870
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Durall de la Fuente, Claudia, et al.
(författare)
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Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt
- 2021
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Ingår i: Microbial Cell Factories. - : BioMed Central (BMC). - 1475-2859. ; 20
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Tidskriftsartikel (refereegranskat)abstract
- Background: Cyanobacteria are promising hosts for the production of various industrially important compounds such as succinate. This study focuses on introduction of the glyoxylate shunt, which is naturally present in only a few cyanobacteria, into Synechocystis PCC 6803. In order to test its impact on cell metabolism, engineered strains were evaluated for succinate accumulation under conditions of light, darkness and anoxic darkness. Each condition was complemented by treatments with 2-thenoyltrifluoroacetone, an inhibitor of succinate dehydrogenase enzyme, and acetate, both in nitrogen replete and deplete medium.Results: We were able to introduce genes encoding the glyoxylate shunt, aceA and aceB, encoding isocitrate lyase and malate synthase respectively, into a strain of Synechocystis PCC 6803 engineered to overexpress phosphoenolpyruvate carboxylase. Our results show that complete expression of the glyoxylate shunt results in higher extracellular succinate accumulation compared to the wild type control strain after incubation of cells in darkness and anoxic darkness in the presence of nitrate. Addition of the inhibitor 2-thenoyltrifluoroacetone increased succinate titers in all the conditions tested when nitrate was available. Addition of acetate in the presence of the inhibitor further increased the succinate accumulation, resulting in high levels when phosphoenolpyruvate carboxylase was overexpressed, compared to control strain. However, the highest succinate titer was obtained after dark incubation of an engineered strain with a partial glyoxylate shunt overexpressing isocitrate lyase in addition to phosphoenolpyruvate carboxylase, with only 2-thenoyltrifluoroacetone supplementation to the medium.Conclusions: Heterologous expression of the glyoxylate shunt with its central link to the tricarboxylic acid cycle (TCA) for acetate assimilation provides insight on the coordination of the carbon metabolism in the cell. Phosphoenolpyruvate carboxylase plays an important role in directing carbon flux towards the TCA cycle.
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| 4. |
- Garrity, N. J., et al.
(författare)
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Evaluation of event and response approaches to estimate the 100-year coastal flood for pacific coast sheltered waters
- 2007
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Ingår i: Coastal Engineering 2006. - : World Scientific Publishing Company. - 9789812706362 ; , s. 1651-1663
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Konferensbidrag (refereegranskat)abstract
- Coastal flooding on the Pacific Coast of the United States typically results from the joint occurrence of relatively high water levels and large waves, which are partially correlated. This paper evaluates three approaches to address the joint probability of water levels and waves (flood forcing events) in estimating coastal flood levels (responses) with a 100-year return period. The event approach involves selecting extreme events that are expected to force a response that approximates or exceeds the 100-year flood response. The two response approaches determine the response probability from a calculated response data set. The approaches were applied to a sheltered waters site to inform the development and implementation of the United States Federal Emergency Management Agency's guidelines for mapping coastal flood hazards on the Pacific Coast. Both event and response approaches can provide adequate results, but each requires varying levels of judgment and has advantages and disadvantages. A hybrid between event and response approaches is identified as a balance of accuracy and efficiency in coastal flood studies.
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