| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Brokking, Peter, 1964-, et al.
(författare)
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SEA in Swedish Transportation Policy-making and Planning : Political Ambitions and Practice
- 2004
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Ingår i: European Environment. - : Wiley-Blackwell. - 0961-0405 .- 1099-0976. ; 14:2, s. 94-104
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Tidskriftsartikel (refereegranskat)abstract
- Since the mid-1990s, the aim to incorporate SEA into Swedish strategic transportation planning has been repeatedly expressed in government documents and directives. To support a successful implementation, efforts have been made to develop tools and methods for the integration of SEA into strategic transportation planning. These efforts concern, for example, the application of SEA in corridor development and long-term transportation planning. The practical experiences of SEA in strategic transportation planning have been influenced by the ambiguity of the scope and structure of SEA. In this paper the process of implementing the SEA in Swedish transportation planning will be reviewed. The review relates to political ambitions and practice, and highlights the methodological development of SEA applications in the Swedish transport sector.
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| 3. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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