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- Dewa Ayu Ratna Dewanti, I., et al.
(författare)
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Bones, scales of Upeneus sulphureus and Osphronemus gouramy increase adhesion and decrease IL-1β expression on monocytes against Streptococcus mutans
- 2020
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Ingår i: Annals of Tropical Medicine and Public Health. - : Wolters Kluwer. - 1755-6783 .- 0974-6005. ; 23:8, s. 1253-1258
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Tidskriftsartikel (refereegranskat)abstract
- Adhesion and IL-1β on cells imunocompetent are important to prevent the spread of Streptococcus mutans infection to the systemic. One of the efforts is to use bones, fish scales Upeneus sulphureus and Osphronemus gouramy, they suspected contain of immunomodulator components. This study aims to analyze the effect of bone, scales of U. sulphureus and O. gouramy on adhesion activity and IL-1β expression on monocytes against S. mutans. This in vivo experimental study using the post test only control group design. Peripheral blood that has been layered with ficoll hypaque, then it added HBSS, Fungizone and Penstripe. The cells were placed in well microtiter plate, then they treated according to groups. Adhesion was stained with Giemza, IL-1β was stained with immunocytochemical. Cells were counted under a light microscope with magnification 400 times per 100 cells. The data obtained were analyzed using ANOVA followed by LSD test. Post test only design and the data obtained were analyzed using ANOVA followed by LSD test. There were no significant differences (p < 0.05) between Bones and scales. They both increase the adhesion of S. mutans and decrease the expression of IL-1β in monocytes. Bone and scales U. ulphureus and O. gouramy increase the recognition of monocytes to S. mutans and inhibit the spread of infection to systemic.
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- Susilawati, I.D.A., et al.
(författare)
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Coffee protects cardiovascular health by maintaining the structure of coronary arterial wall intimal collagen
- 2020
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Ingår i: Coffee Science. - : Editora UFLA. - 1809-6875 .- 1984-3909. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed to determine whether coffee consumption affects the structure of coronary arterial wall and protects against coronary artery disease (CAD) in atherosclerotic rat model induced by periodontitis. Rats (n=21) were divided into three groups (i) Coffee group (periodontitis + coffee), (ii) Peri-odontitis group (no coffee), and (iii) Control group (no periodontitis, no coffee). A single dose of coffee suspension (representing one cup) was given daily by stomach sondation to the rats in the coffee group. The experiment was conducted for 5 wk. At the completion of the experiment, all of rats were sac-rificed. Their hearts containing coronary arteries were removed and analyzed by histochemistry assay. In addition, the serum level of collagen degrading enzymes matrix metalloproteinase–2 (MMP–2) was also analyzed using Enzyme–link immunosorbent assay (Elisa). Results demonstrated that coronary atherosclerotic lesions including atheroma, stenosis, and vascular occlusion were rarely identified in the coffee group. The coronary arterial wall demonstrated relatively symmetrical intima-media thickness (IMT) and the lumen diameter remained adequate for blood flow. The intimal collagen was intact, dense and thick. MMP–2 level was significantly lower (P < 0.05) in the coffee group. In conclusion, coffee maintained the structure of coronary arterial wall particularly the intimal collagen, providing protection against CAD. This might also mediate the vascular resistance against rupture and thrombosis that might precipitate the occurrence of acute coronary syndrome (ACS).
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