| 1. |
- Fasterius, Erik, et al.
(författare)
-
A novel RNA sequencing data analysis method for cell line authentication
- 2017
-
Ingår i: PLOS ONE. - : PUBLIC LIBRARY SCIENCE. - 1932-6203. ; 12:2
-
Tidskriftsartikel (refereegranskat)abstract
- We have developed a novel analysis method that can interrogate the authenticity of biological samples used for generation of transcriptome profiles in public data repositories. The method uses RNA sequencing information to reveal mutations in expressed transcripts and subsequently confirms the identity of analysed cells by comparison with publicly available cell-specific mutational profiles. Cell lines constitute key model systems widely used within cancer research, but their identity needs to be confirmed in order to minimise the influence of cell contaminations and genetic drift on the analysis. Using both public and novel data, we demonstrate the use of RNA-sequencing data analysis for cell line authentication by examining the validity of COLO205, DLD1, HCT15, HCT116, HKE3, HT29 and RKO colorectal cancer cell lines. We successfully authenticate the studied cell lines and validate previous reports indicating that DLD1 and HCT15 are synonymous. We also show that the analysed HKE3 cells harbour an unexpected KRAS-G13D mutation and confirm that this cell line is a genuine KRAS dosage mutant, rather than a true isogenic derivative of HCT116 expressing only the wild type KRAS. This authentication method could be used to revisit the numerous cell line based RNA sequencing experiments available in public data repositories, analyse new experiments where whole genome sequencing is not available, as well as facilitate comparisons of data from different experiments, platforms and laboratories.
|
|
| 2. |
- Michele Felicetti, Alberto, et al.
(författare)
-
Being a start-upper in Italy : Motivations, obstacles and success factors
- 2016
-
Ingår i: Proceedings of the 11th International Forum on Knowledge Asset Dynamics. - : Proceedings IFKAD.... - 9788896687093 ; , s. 1370-1383
-
Konferensbidrag (refereegranskat)abstract
- Purpose - In modern industrial economies, start-up companies are taking an increasingly central role in several government initiatives as a basic engine for socioeconomic development. The scientific literature shows that the success of these business initiatives depends on several factors. In addition to the intrinsic quality of each initiative, several researchers agree in the importance of factors related to the context in which start-ups live and operate. This study aims to analyze motivations, obstacles and success factors of start-up companies in Italy.Design/methodology/approach – First, we carried out a systematic literature review on start-up companies domain. In order to answer to the above-mentioned research questions, we collected data through an empirical study based on semi-structured interviews. Survey was conducted in June 2015. The questionnaire was completed by 348 start-up companies, with a response rate of 21,19%. Data were analysed through 11th International Forum on Knowledge Asset Dynamics quantitative statistical techniques, such as factor analysis and ANOVA, to identify goals and critical success factors for Italian Start-uppers.Originality/value - Empirical studies revealed the presence of several regions on a global scale, characterized by the presence of successful start-ups. The presence of geographical areas globally acknowledged as real technological hubs (e.g. Silicon Valley) is due to the presence of a set of conditions that, in the system, are extremely favorable to their development. Although many researches provide frameworks to analyze start-ups ecosystems at a glance, we highlight that literature lacks of an analysis of Start-uppers profiles and goals, as well an identification of the critical success factors, according the Start-uppers' perspective. Moreover, although there is already some significant body of knowledge on Italian Start-ups Panorama, many of these works are in the form of journalistic and non-academic books and reports.Practical implications – In Europe, too many promising high tech start-ups do not manage to grow and scale up at international level. Italy is characterized by a constellation of small businesses that, despite their peculiarities, have great difficulty in competing in the global market. Although the start-up phenomenon is growing, the Italian situation is still in its infancy, with many start-up with a small number of employees and still in early stage. The identification of the characteristics of the Italian start-up companies will allow a better understanding of this entrepreneurial phenomenon, supporting the design of appropriate innovation policies and regional industry development.
|
|
