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A global benchmark study using affinity-based biosensors

Rich, Rebecca L. (author)
Papalia, Giuseppe A. (author)
Flynn, Peter J. (author)
show more...
Furneisen, Jamie (author)
Quinn, John (author)
Klein, Joshua S. (author)
Katsamba, Phini S. (author)
Waddell, M. Brent (author)
Scott, Michael (author)
Thompson, Joshua (author)
Berlier, Judie (author)
Corry, Schuyler (author)
Baltzinger, Mireille (author)
Zeder-Lutzi, Gabrielle (author)
Schoenemann, Andreas (author)
Clabbers, Anca (author)
Wieckowski, Sebastien (author)
Murphy, Mary M. (author)
Page, Phillip (author)
Ryan, Thomas E. (author)
Duffner, Jay (author)
Ganguly, Tanmoy (author)
Corbin, John (author)
Gautam, Satyen (author)
Anderluh, Gregor (author)
Bavdek, Andrej (author)
Reichmann, Dana (author)
Yadav, Satya P. (author)
Hommema, Eric (author)
Pol, Ewa (author)
Drake, Andrew (author)
Klakamp, Scott (author)
Chapman, Trevor (author)
Kernaghan, Dawn (author)
Miller, Ken (author)
Schuman, Jason (author)
Lindquist, Kevin (author)
Herlihy, Kara (author)
Murphy, Michael B. (author)
Bohnsack, Richard (author)
Andrien, Bruce (author)
Brandani, Pietro (author)
Terwey, Danny (author)
Millican, Rohn (author)
Darling, Ryan J. (author)
Wang, Liann (author)
Carter, Quincy (author)
Dotzlaf, Joe (author)
Lopez-Sagaseta, Jacinto (author)
Campbell, Islay (author)
Torreri, Paola (author)
Hoos, Sylviane (author)
England, Patrick (author)
Liu, Yang (author)
Abdiche, Yasmina (author)
Malashock, Daniel (author)
Pinkerton, Alanna (author)
Wong, Melanie (author)
Lafer, Eileen (author)
Hinck, Cynthia (author)
Thompson, Kevin (author)
Di Primo, Carmelo (author)
Joyce, Alison (author)
Brooks, Jonathan (author)
Torta, Federico (author)
Hagel, Anne Birgitte Bagge (author)
Krarup, Janus (author)
Pass, Jesper (author)
Ferreira, Monica (author)
Södertörns högskola,Molekylärbiologi
Shikov, Sergei (author)
Mikolajczyk, Malgorzata (author)
Abe, Yuki (author)
Barbato, Gaetano (author)
Giannetti, Anthony M. (author)
Krishnamoorthy, Ganeshram (author)
Beusink, Bianca (author)
Satpaev, Daulet (author)
Tsang, Tiffany (author)
Fang, Eric (author)
Partridge, James (author)
Brohawn, Stephen (author)
Horn, James (author)
Pritsch, Otto (author)
Obal, Gonzalo (author)
Nilapwar, Sanjay (author)
Busby, Ben (author)
Gutierrez-Sanchez, Gerardo (author)
Das Gupta, Ruchira (author)
Canepa, Sylvie (author)
Witte, Krista (author)
Nikolovska-Coleska, Zaneta (author)
Cho, Yun Hee (author)
D'Agata, Roberta (author)
Schlick, Kristian (author)
Calvert, Rosy (author)
Munoz, Eva M. (author)
Hernaiz, Maria Jose (author)
Bravman, Tsafir (author)
Dines, Monica (author)
Yang, Min-Hsiang (author)
Puskas, Agnes (author)
Boni, Erica (author)
Li, Jiejin (author)
Wear, Martin (author)
Grinberg, Asya (author)
Baardsnes, Jason (author)
Dolezal, Olan (author)
Gainey, Melicia (author)
Anderson, Henrik (author)
Peng, Jinlin (author)
Lewis, Mark (author)
Spies, Peter (author)
Trinh, Quyhn (author)
Bibikov, Sergei (author)
Raymond, Jill (author)
Yousef, Mohammed (author)
Chandrasekaran, Vidya (author)
Feng, Yuguo (author)
Emerick, Anne (author)
Mundodo, Suparna (author)
Guimaraes, Rejane (author)
McGirr, Katy (author)
Li, Yue-Ji (author)
Hughes, Heather (author)
Mantz, Hubert (author)
Skrabana, Rostislav (author)
Witmer, Mark (author)
Ballard, Joshua (author)
Martin, Loic (author)
Skladal, Petr (author)
Korza, George (author)
Laird-Offringa, Ite (author)
Lee, Charlene S. (author)
Khadir, Abdelkrim (author)
Podlaski, Frank (author)
Neuner, Phillippe (author)
Rothacker, Julie (author)
Rafique, Ashique (author)
Dankbar, Nico (author)
Kainz, Peter (author)
Gedig, Erk (author)
Vuyisich, Momchilo (author)
Boozer, Christina (author)
Ly, Nguyen (author)
Toews, Mark (author)
Uren, Aykut (author)
Kalyuzhniy, Oleksandr (author)
Lewis, Kenneth (author)
Chomey, Eugene (author)
Pak, Brian J. (author)
Myszka, David G. (author)
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 (creator_code:org_t)
Elsevier BV, 2009
2009
English.
In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor
Rich, Rebecca L.
Papalia, Giusepp ...
Flynn, Peter J.
Furneisen, Jamie
Quinn, John
Klein, Joshua S.
show more...
Katsamba, Phini ...
Waddell, M. Bren ...
Scott, Michael
Thompson, Joshua
Berlier, Judie
Corry, Schuyler
Baltzinger, Mire ...
Zeder-Lutzi, Gab ...
Schoenemann, And ...
Clabbers, Anca
Wieckowski, Seba ...
Murphy, Mary M.
Page, Phillip
Ryan, Thomas E.
Duffner, Jay
Ganguly, Tanmoy
Corbin, John
Gautam, Satyen
Anderluh, Gregor
Bavdek, Andrej
Reichmann, Dana
Yadav, Satya P.
Hommema, Eric
Pol, Ewa
Drake, Andrew
Klakamp, Scott
Chapman, Trevor
Kernaghan, Dawn
Miller, Ken
Schuman, Jason
Lindquist, Kevin
Herlihy, Kara
Murphy, Michael ...
Bohnsack, Richar ...
Andrien, Bruce
Brandani, Pietro
Terwey, Danny
Millican, Rohn
Darling, Ryan J.
Wang, Liann
Carter, Quincy
Dotzlaf, Joe
Lopez-Sagaseta, ...
Campbell, Islay
Torreri, Paola
Hoos, Sylviane
England, Patrick
Liu, Yang
Abdiche, Yasmina
Malashock, Danie ...
Pinkerton, Alann ...
Wong, Melanie
Lafer, Eileen
Hinck, Cynthia
Thompson, Kevin
Di Primo, Carmel ...
Joyce, Alison
Brooks, Jonathan
Torta, Federico
Hagel, Anne Birg ...
Krarup, Janus
Pass, Jesper
Ferreira, Monica
Shikov, Sergei
Mikolajczyk, Mal ...
Abe, Yuki
Barbato, Gaetano
Giannetti, Antho ...
Krishnamoorthy, ...
Beusink, Bianca
Satpaev, Daulet
Tsang, Tiffany
Fang, Eric
Partridge, James
Brohawn, Stephen
Horn, James
Pritsch, Otto
Obal, Gonzalo
Nilapwar, Sanjay
Busby, Ben
Gutierrez-Sanche ...
Das Gupta, Ruchi ...
Canepa, Sylvie
Witte, Krista
Nikolovska-Coles ...
Cho, Yun Hee
D'Agata, Roberta
Schlick, Kristia ...
Calvert, Rosy
Munoz, Eva M.
Hernaiz, Maria J ...
Bravman, Tsafir
Dines, Monica
Yang, Min-Hsiang
Puskas, Agnes
Boni, Erica
Li, Jiejin
Wear, Martin
Grinberg, Asya
Baardsnes, Jason
Dolezal, Olan
Gainey, Melicia
Anderson, Henrik
Peng, Jinlin
Lewis, Mark
Spies, Peter
Trinh, Quyhn
Bibikov, Sergei
Raymond, Jill
Yousef, Mohammed
Chandrasekaran, ...
Feng, Yuguo
Emerick, Anne
Mundodo, Suparna
Guimaraes, Rejan ...
McGirr, Katy
Li, Yue-Ji
Hughes, Heather
Mantz, Hubert
Skrabana, Rostis ...
Witmer, Mark
Ballard, Joshua
Martin, Loic
Skladal, Petr
Korza, George
Laird-Offringa, ...
Lee, Charlene S.
Khadir, Abdelkri ...
Podlaski, Frank
Neuner, Phillipp ...
Rothacker, Julie
Rafique, Ashique
Dankbar, Nico
Kainz, Peter
Gedig, Erk
Vuyisich, Momchi ...
Boozer, Christin ...
Ly, Nguyen
Toews, Mark
Uren, Aykut
Kalyuzhniy, Olek ...
Lewis, Kenneth
Chomey, Eugene
Pak, Brian J.
Myszka, David G.
show less...
About the subject
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Biochemistry and ...
Articles in the publication
Analytical Bioch ...
By the university
Södertörn University

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