| 1. |
- Almqvist, Andreas, et al.
(författare)
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An Abbott curve based rough surface contact mechanics approach
- 2005
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Ingår i: Proceedings of the World Tribology Congress III - 2005. - New York : American Society of Mechanical Engineers. ; , s. 397-398
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Konferensbidrag (refereegranskat)abstract
- In this way all the height information of the surface profile is preserved and not only a few parameters, like Ra, Rq, Rz, Rsk, etc. The aim of this work is to investigate how classes of surfaces based on a single Abbott curve perform in terms of contact mechanical parameters like the real area of contact. The result shows that surfaces taken from a class of random surfaces generated from a specific Abbott curve behaves similar in a contact mechanics simulation. That is, the distribution of for example the real area of contact within such a class is compact, having a small deviation from its mean.This implies that it is possible to simulate classes of surfaces based on Abbott curves and to use the results to predict contact mechanical properties of real surface topographies.
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| 2. |
- Almqvist, Andreas, et al.
(författare)
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Development of a lubrication simulation model
- 2009
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Ingår i: Svenska mekanikdagarna. - Stockholm : Svenska nationalkommittén för mekanik. ; , s. 74-
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 3. |
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| 4. |
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| 5. |
- Almqvist, Andreas, et al.
(författare)
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On the dry elasto-plastic contact of nominally flat surfaces
- 2007
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Ingår i: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 40:4, s. 574-579
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Tidskriftsartikel (refereegranskat)abstract
- A model to be used for numerical simulation of the contact of linear elastic perfectly plastic rough surfaces was developed. Energy dissipation due to plastic deformation is taken into account. Spectral theory and an FFT-techique are used to facilitate the numerical solution process. Results of simulations using four two-dimensional profiles with different topographies in contact with a rigid plane for a number loads are reported. From the results it is clear that the real area of contact (Ar) changes almost linearly with load and is only slightly affected by the difference in topography. A plasticity index is defined as the ratio of plastically deformed area (Ap) and Ar. Plastic deformation occurs even at low loads and there is a significant difference in plasticity index between the surface profiles considered. An investigation on how the spectral content of the surface profile influences the results presented is also performed. This is to ensure that the metrological limitations of the optical profilerused to measure the surfaces do not have a significant influence. It is concluded that the highest frequencies of the measured profile have a negligible influence on the real area of contact.
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| 6. |
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| 7. |
- Aurelius, Oskar, et al.
(författare)
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The Crystal Structure of Thermotoga maritima Class III Ribonucleotide Reductase Lacks a Radical Cysteine Pre-Positioned in the Active Site
- 2015
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:7
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Tidskriftsartikel (refereegranskat)abstract
- Ribonucleotide reductases (RNRs) catalyze the reduction of ribonucleotides to deoxyribonucleotides, the building blocks for DNA synthesis, and are found in all but a few organisms. RNRs use radical chemistry to catalyze the reduction reaction. Despite RNR having evolved several mechanisms for generation of different kinds of essential radicals across a large evolutionary time frame, this initial radical is normally always channelled to a strictly conserved cysteine residue directly adjacent to the substrate for initiation of substrate reduction, and this cysteine has been found in the structures of all RNRs solved to date. We present the crystal structure of an anaerobic RNR from the extreme thermophile Thermotoga maritima (tmNrdD), alone and in several complexes, including with the allosteric effector dATP and its cognate substrate CTP. In the crystal structure of the enzyme as purified, tmNrdD lacks a cysteine for radical transfer to the substrate pre-positioned in the active site. Nevertheless activity assays using anaerobic cell extracts from T. maritima demonstrate that the class III RNR is enzymatically active. Other genetic and microbiological evidence is summarized indicating that the enzyme is important for T. maritima. Mutation of either of two cysteine residues in a disordered loop far from the active site results in inactive enzyme. We discuss the possible mechanisms for radical initiation of substrate reduction given the collected evidence from the crystal structure, our activity assays and other published work. Taken together, the results suggest either that initiation of substrate reduction may involve unprecedented conformational changes in the enzyme to bring one of these cysteine residues to the expected position, or that alternative routes for initiation of the RNR reduction reaction may exist. Finally, we present a phylogenetic analysis showing that the structure of tmNrdD is representative of a new RNR subclass IIIh, present in all Thermotoga species plus a wider group of bacteria from the distantly related phyla Firmicutes, Bacteroidetes and Proteobacteria.
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| 8. |
- Berggren, Gustav, et al.
(författare)
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Compounds with capacity to quench the tyrosyl radical in Pseudomonas aeruginosa ribonucleotide reductase
- 2019
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Ingår i: Journal of Biological Inorganic Chemistry. - : Springer Science and Business Media LLC. - 0949-8257 .- 1432-1327. ; 24:6, s. 841-848
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Tidskriftsartikel (refereegranskat)abstract
- Ribonucleotide reductase (RNR) has been extensively probed as a target enzyme in the search for selective antibiotics. Here we report on the mechanism of inhibition of nine compounds, serving as representative examples of three different inhibitor classes previously identified by us to efficiently inhibit RNR. The interaction between the inhibitors and Pseudomonas aeruginosa RNR was elucidated using a combination of electron paramagnetic resonance spectroscopy and thermal shift analysis. All nine inhibitors were found to efficiently quench the tyrosyl radical present in RNR, required for catalysis. Three different mechanisms of radical quenching were identified, and shown to depend on reduction potential of the assay solution and quaternary structure of the protein complex. These results form a good foundation for further development of P. aeruginosa selective antibiotics. Moreover, this study underscores the complex nature of RNR inhibition and the need for detailed spectroscopic studies to unravel the mechanism of RNR inhibitors.
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| 9. |
- Björling, Marcus
(författare)
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Friction in elasto hydrodynamically lubricated contacts : the influence of speed and slide to roll ratio
- 2011
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Reducing losses in transmissions has become a high priority in the automotive market during recent years, mainly due to environmental concerns leading to regulations placed on the automotive industry to drive the development of vehicles with lower fuel consumption and CO2 emissions. Rising fuel prices and increasing environmental concerns have also made customers more prone to purchase more fuel efficient vehicles. In addition to the fuel savings that could be achieved by increased efficiency of transmissions there are other benefits as well. A more efficient transmission will in general generate less heat, and experience less wear. This will lead to fewer failures, longer service life of components, and possibly longer service intervals. Furthermore this implies a possibility to reduce coolant components, thus reducing the total weight of the system, leading to a further decrease in consumption and a lower impact on the environment due to a reduction of material usage. A low weight design is also beneficial for vehicle dynamics and handling. In addition to the automotive market, gears are extensively used in many other fields, such as wind power and industry. In some cases a substantial part of the losses in a gear transmission is attributed to gear contact friction due to sliding and rolling between the gear teeth. To better understand the contact friction phenomena in gears an experimental apparatus capable of running under similar conditions to gears is chosen. By using a ball on disc test device the contact friction can be measured in a broad range of speeds and slide to roll ratios (SRR). The results are presented as a 3D friction map which can be divided into four different regions; Linear, Non-linear, mixed and thermal. In each of these regions different mechanisms are influencing the coefficient of friction. Several tests have been conducted with different lubricants, EP- additive packages, operating temperatures, surface roughness and coatings. The method gives a good overview, a system fingerprint, of the frictional behaviour for a specific system in a broad operating range. By observing results for different systems, it is possible to identify how different changes will influence the coefficient of friction in different regimes, and therefore optimize the system depending on operating conditions. Among other things the tests have shown that reducing base oil viscosity increases contact friction in most operating conditions, introducing an earlier transition from full film to mixed lubrication, and increasing full film friction in many cases with high sliding speeds. An increase in operating temperature could both increase, and decrease the coefficient of friction depending on running conditions. Introducing smoother surfaces reduces the coefficient of friction at lower entrainment speeds since thinner lubricant films are required to avoid asperity collitions. By applying a DLC coating on one or both surfaces in a EHL contact, the friction coefficient is shown to decrease, even in the full film regime.
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| 10. |
- Crona, Mikael, et al.
(författare)
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A Rare Combination of Ribonucleotide Reductases in the Social Amoeba Dictyostelium discoideum
- 2013
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 288:12, s. 8198-8208
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Tidskriftsartikel (refereegranskat)abstract
- Ribonucleotide reductases (RNRs) catalyze the only pathway for de novo synthesis of deoxyribonucleotides needed for DNA replication and repair. The vast majority of eukaryotes encodes only a class I RNR, but interestingly some eukaryotes, including the social amoeba Dictyostelium discoideum, encode both a class I and a class II RNR. The amino acid sequence of the D. discoideum class I RNR is similar to other eukaryotic RNRs, whereas that of its class II RNR is most similar to the monomeric class II RNRs found in Lactobacillus spp. and a few other bacteria. Here we report the first study of RNRs in a eukaryotic organism that encodes class I and class II RNRs. Both classes of RNR genes were expressed in D. discoideum cells, although the class I transcripts were more abundant and strongly enriched during mid-development compared with the class II transcript. The quaternary structure, allosteric regulation, and properties of the diiron-oxo/radical cofactor of D. discoideum class I RNR are similar to those of the mammalian RNRs. Inhibition of D. discoideum class I RNR by hydroxyurea resulted in a 90% reduction in spore formation and decreased the germination viability of the surviving spores by 75%. Class II RNR could not compensate for class I inhibition during development, and an excess of vitamin B12 coenzyme, which is essential for class II activity, did not improve spore formation. We suggest that class I is the principal RNR during D. discoideum development and growth and is important for spore formation, possibly by providing dNTPs for mitochondrial replication.
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