| 1. |
- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Weinstein, John N., et al.
(författare)
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The cancer genome atlas pan-cancer analysis project
- 2013
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:10, s. 1113-1120
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Forskningsöversikt (refereegranskat)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
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| 3. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 4. |
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| 5. |
- Ma, Yang, et al.
(författare)
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Towards real-time whisker tracking in rodents for studying sensorimotor disorders
- 2017
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Ingår i: Proceedings - 2017 17th International Conference on Embedded Computer Systems: Architectures, Modeling, and Simulation, SAMOS 2017. ; 2018-January, s. 137-145
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Konferensbidrag (refereegranskat)abstract
- The rodent whisker system is a prominent experimental subject for the study of sensorimotor integration and active sensing. As a result of improved video-recording technology and progressively better neurophysiological methods, there is now the prospect of precisely analyzing the intact vibrissal sensori-motor system. The vibrissae and snout analyzer (ViSA), a widely used algorithm based on computer vision and image processing, has been proven successful for tracking and quantifying rodent sensorimotor behavior, but at a great cost in processing time. In order to accelerate this offline algorithm and eventually employ it for online whisker tracking (less than 1 ms/frame latency), we have explored various optimizations and acceleration platforms, including OpenMP multithreading, NVidia GPUs and Maxeler Dataflow Engines. Our experimental results indicate that the optimal solution for an offline implementation of ViSA is currently the OpenMP-based CPU execution. By using 16 CPU threads, we achieve more than 4,500x speedup compared to the original Matlab serial version, resulting in an average processing latency of 1.2 ms/frame, which is a solid step towards real-time (and online) tracking. Analysis shows that running the algorithm on a 32-thread-enabled machine can reduce this number to 0.72 ms/frame, thereby enabling real-time performance. This will allow direct interaction with the whisker system during behavioral experiments. In conclusion, our approach shows that a combination of software optimizations and the careful selection of hardware platform yields the best performance increase.
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| 6. |
- Messner, Christoph B., et al.
(författare)
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Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection
- 2020
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Ingår i: Cell Systems. - : Elsevier BV. - 2405-4712 .- 2405-4720. ; 11:1, s. 11-24.E4
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Tidskriftsartikel (refereegranskat)abstract
- The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pro-inflammatory factors upstream and downstream of interleukin 6. All protocols and software for implementing our approach are freely available. In total, this work supports the development of routine proteomic assays to aid clinical decision making and generate hypotheses about potential COVID-19 therapeutic targets.
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| 7. |
- Demichev, Vadim, et al.
(författare)
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A time-resolved proteomic and prognostic map of COVID-19
- 2021
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Ingår i: Cell Systems. - : Elsevier BV. - 2405-4712 .- 2405-4720. ; 12:8, s. 780-794.e7
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Tidskriftsartikel (refereegranskat)abstract
- COVID-19 is highly variable in its clinical presentation, ranging from asymptomatic infection to severe organ damage and death. We characterized the time-dependent progression of the disease in 139 COVID-19 inpatients by measuring 86 accredited diagnostic parameters, such as blood cell counts and enzyme activities, as well as untargeted plasma proteomes at 687 sampling points. We report an initial spike in a systemic inflammatory response, which is gradually alleviated and followed by a protein signature indicative of tissue repair, metabolic reconstitution, and immunomodulation. We identify prognostic marker signatures for devising risk-adapted treatment strategies and use machine learning to classify therapeutic needs. We show that the machine learning models based on the proteome are transferable to an independent cohort. Our study presents a map linking routinely used clinical diagnostic parameters to plasma proteomes and their dynamics in an infectious disease.
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| 8. |
- Flannagan, Carol. A. C., et al.
(författare)
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Comparing motor-vehicle crash risk of EU and US vehicles
- 2018
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Ingår i: Accident Analysis and Prevention. - : Elsevier Ltd. - 0001-4575 .- 1879-2057.
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Tidskriftsartikel (refereegranskat)abstract
- This study examined the hypotheses that passenger vehicles meeting European Union (EU) safety standards have similar crashworthiness to United States (US) -regulated vehicles in the US driving environment, and vice versa. The first step involved identifying appropriate databases of US and EU crashes that include in-depth crash information, such as estimation of crash severity using Delta-V and injury outcome based on medical records. The next step was to harmonize variable definitions and sampling criteria so that the EU data could be combined and compared to the US data using the same or equivalent parameters. Logistic regression models of the risk of a Maximum injury according to the Abbreviated Injury Scale of 3 or greater, or fatality (MAIS3+F) in EU-regulated and US-regulated vehicles were constructed. The injury risk predictions of the EU model and the US model were each applied to both the US and EU standard crash populations. Frontal, near-side, and far-side crashes were analyzed together (termed "front/side crashes") and a separate model was developed for rollover crashes.For the front/side model applied to the US standard population, the mean estimated risk for the US-vehicle model is 0.035 (sd = 0.012), and the mean estimated risk for the EU-vehicle model is 0.023 (sd = 0.016). When applied to the EU front/side population, the US model predicted a 0.065 risk (sd = 0.027), and the EU model predicted a 0.052 risk (sd = 0.025). For the rollover model applied to the US standard population, the US model predicted a risk of 0.071 (sd = 0.024), and the EU model predicted 0.128 risk (sd = 0.057). When applied to the EU rollover standard population, the US model predicted a 0.067 risk (sd = 0.024), and the EU model predicted 0.103 risk (sd = 0.040).The results based on these methods indicate that EU vehicles most likely have a lower risk of MAIS3+F injury in front/side impacts, while US vehicles most likely have a lower risk of MAIS3+F injury in llroovers. These results should be interpreted with an understanding of the uncertainty of the estimates, the study limitations, and our recommendations for further study detailed in the report.
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| 9. |
- Flannagan, Carol A.C., et al.
(författare)
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Mutual Recognition Methodology Development
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Phase 1 of the Mutual Recognition Methodology Development (MRMD) project developed an approach to statistical modeling and analysis of field data to address the state of evidence relevant to mutual recognition of automotive safety regulations. Specifically, the report describes a methodology that can be used to measure evidence for the hypothesis that vehicles meeting EU safety standards would perform similarly to US-regulated vehicles in the US driving environment, and that vehicles meeting US safety standards would perform similarly to EU-regulated vehicles in the EU driving environment. As part of the project, we assessed the availability and contents of crash datasets from the US and the EU, as well as their collective ability to support the proposed statistical methodology.The report describes a set of three statistical approaches to “triangulate” evidence regarding similarity or differences in crash and injury risk associated with EU- and US-regulated vehicles. Approach 1, Seemingly Unrelated Regression, tests whether the models are identical and will also assess the capability of the data analysis to detect differences in the models, if differences exist.Approach 2, Consequences of Best Models, uses logistic regression to develop two separate models, one for EU risk and one for US risk, as a function of a set of predictors (i.e., crash, vehicle, and occupant conditions). The two models will then be exercised on a standard population for the EU and a standard population for the US. Approach 3, Evidence for Consequences, turns the question aroundto measures the overall evidence for each of a set of possible conclusions. Each conclusion is characterized by a range of relative risk on a single population. Evidence is measured using a weighted average of likelihoods for a large group of models that produce the same outcome. That evidence is then compared using Bayes Factors.
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| 10. |
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