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| 2. |
- Frisk, Thomas, et al.
(författare)
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An integrated QCM-based narcotics sensing microsystem
- 2008
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Ingår i: Lab on a Chip. - : RSC Publishing. - 1473-0197 .- 1473-0189. ; 8:10, s. 1648-1657
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Tidskriftsartikel (refereegranskat)abstract
- We present the design, fabrication and successful testing of a 14 x 14 x 4 mm(3) integrated electronic narcotics sensing system which consists of only four parts. The microsystem absorbs airborne narcotics molecules and performs a liquid assay using an integrated quartz crystal microbalance (QCM). A vertically conductive double-sided adhesive foil (VCAF) was used and studied as a novel material for LOC and MEMS applications and provides easy assembly, electrical contacting and liquid containment. The system was tested for measuring cocaine and ecstasy, with successful detection of amounts as small as 100 ng and 200 ng, respectively These levels are of interest in security activities in customs, prisons and by the police.
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| 3. |
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| 4. |
- Sandström, Niklas, et al.
(författare)
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ELECTROHYDRODYNAMIC ENHANCED TRANSPORT AND TRAPPING OF AIRBORNE PARTICLES TO A MICROFLUIDIC AIR-LIQUID INTERFACE
- 2008
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Ingår i: 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008). - : IEEE conference proceedings. - 9781424417926 ; , s. 595-598
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Konferensbidrag (refereegranskat)abstract
- We introduce a novel approach for greatly improved transport and trapping of airborne sample to a microfluidic analysis system by integrating an electrohydrodynamic (EHD) air pump with a microfluidic air-liquid interface. In our system, a negative corona discharge partially ionizes the air around a sharp electrode tip while the electrostatic field accelerates airborne particles towards an electrically grounded liquid surface, where they absorb. The air-liquid interface is fixated at the microscale pores of a perforated silicon diaphragm, each pore functioning as a static Laplace valve. Our system was experimentally tested using airborne smoke particles of ammonium chloride and aqueous salt solution as the liquid. We measured that EHD enhanced transport of the particles from the air into the liquid is enhanced over 130 times compared to passive trapping.
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| 5. |
- Sandström, Niklas, 1981-, et al.
(författare)
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Microsystems for Airborne Sample Detection
- 2008
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Ingår i: Mini-symposium on Opto-Microfluidics for Biological Large Scale Integration.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 6. |
- Sandström, Niklas, 1981-, et al.
(författare)
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Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip
- 2022
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Ingår i: CELL REPORTS METHODS. - : Elsevier BV. - 2667-2375. ; 2:7
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Tidskriftsartikel (refereegranskat)abstract
- Here, we present a methodology based on multiplexed fluorescence screening of two-or three-dimensional cell cultures in a newly designed multichambered microwell chip, allowing direct assessment of drug or im-mune cell cytotoxic efficacy. We establish a framework for cell culture, formation of tumor spheroids, fluores-cence labeling, and imaging of fixed or live cells at various magnifications directly in the chip together with data analysis and interpretation. The methodology is demonstrated by drug cytotoxicity screening using ovarian and non-small cell lung cancer cells and by cellular cytotoxicity screening targeting tumor spheroids of renal carcinoma and ovarian carcinoma with natural killer cells from healthy donors. The miniaturized format allowing long-term cell culture, efficient screening, and high-quality imaging of small sample volumes makes this methodology promising for individualized cytotoxicity tests for precision medicine.
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| 7. |
- van Ooijen, Hanna, et al.
(författare)
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Screening and high-resolution imaging of dynamic single-cell responses in 2D and 3D using a novel disposable microwell chip
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Single-cell assays are developed as novel tools for the identification and characterization of individual cell responses. Some methods provide snapshots of the phenotype of the cell, such as flow cytometry and single-cell RNA sequencing, whereas others, almost exclusively microscopy-based, can be used for longitudinal studies of individual cells. In the present study, we developed an easy-to-use, disposable, thermoplastic microwell chip, designed to support screening and high-resolution imaging of single-cell behavior in two-and three-dimensional cell cultures. We confirm its excellent optical properties and provide simple protocols for efficient long-term cell culture of suspension and adherent cells, the latter grown either as monolayers or as hundreds of single, uniformly-sized spheroids. We demonstrate the applicability of the system for single-cell analysis by correlating the dynamic cytotoxic response of single immune cells to their intracellular cytolytic load at the end of the assay. Additionally, we illustrate highly multiplex cytotoxicity screening of tumor spheroids in the chip, comparing the effect of environment cues characteristic of the tumor microenvironment on NK cell-induced killing. Following the functional screening, we perform high-resolution 3D immunofluorescent imaging of infiltrating NK cells within the spheroid volumes.
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| 8. |
- Verron, Quentin, et al.
(författare)
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Isolation of individual natural killer cells from deep microwell arrays based on functional screening
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Natural killer (NK) cells exhibit functional heterogeneity beyond what is resolved by classical definitions of subpopulations based on cell surface expression of receptors. To develop efficient NK cell-based immunotherapies, we need to understand the parameters that dictate their response to activating and inhibiting stimuli, and environmental factors. For this, new methods are required that can connect NK cell function, e.g. high cytotoxic potential, to molecular or genetic signatures. In this study, we build upon a previously developed microwell chip platform for functional single-cell screening of NK cell behavior, and integrate it into a semi-automated workflow for single-cell identification and isolation. We characterize its performance at retrieving and releasing beads from deep microwells and demonstrate its potential for single NK cell isolation with intact viability. This platform offers new opportunities both for improved understanding of NK cell biology, and for tailoring immunotherapy products with selected NK cell responses.
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