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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 4. |
- Blunden, Jessica, et al.
(författare)
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State of the Climate in 2012
- 2013
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Ingår i: Bulletin of The American Meteorological Society - (BAMS). - 0003-0007 .- 1520-0477. ; 94:8, s. S1-S258
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Tidskriftsartikel (refereegranskat)abstract
- For the first time in serveral years, the El Nino-Southern Oscillation did not dominate regional climate conditions around the globe. A weak La Ni a dissipated to ENSOneutral conditions by spring, and while El Nino appeared to be emerging during summer, this phase never fully developed as sea surface temperatures in the eastern conditions. Nevertheless, other large-scale climate patterns and extreme weather events impacted various regions during the year. A negative phase of the Arctic Oscillation from mid-January to early February contributed to frigid conditions in parts of northern Africa, eastern Europe, and western Asia. A lack of rain during the 2012 wet season led to the worst drought in at least the past three decades for northeastern Brazil. Central North America also experienced one of its most severe droughts on record. The Caribbean observed a very wet dry season and it was the Sahel's wettest rainy season in 50 years. Overall, the 2012 average temperature across global land and ocean surfaces ranked among the 10 warmest years on record. The global land surface temperature alone was also among the 10 warmest on record. In the upper atmosphere, the average stratospheric temperature was record or near-record cold, depending on the dataset. After a 30-year warming trend from 1970 to 1999 for global sea surface temperatures, the period 2000-12 had little further trend. This may be linked to the prevalence of La Ni a-like conditions during the 21st century. Heat content in the upper 700 m of the ocean remained near record high levels in 2012. Net increases from 2011 to 2012 were observed at 700-m to 2000-m depth and even in the abyssal ocean below. Following sharp decreases in to the effects of La Ni a, sea levels rebounded to reach records highs in 2012. The increased hydrological cycle seen in recent years continued, with more evaporation in drier locations and more precipitation in rainy areas. In a pattern that has held since 2004, salty areas of the ocean surfaces and subsurfaces were anomalously salty on average, while fresher areas were anomalously fresh. Global tropical cyclone activity during 2012 was near average, with a total of 84 storms compared with the 1981-2010 average of 89. Similar to 2010 and 2011, the North Atlantic was the only hurricane basin that experienced above-normal activity. In this basin, Sandy brought devastation to Cuba and parts of the eastern North American seaboard. All other basins experienced either near-or below-normal tropical cyclone activity. Only three tropical cyclones reached Category 5 intensity-all in Bopha became the only storm in the historical record to produce winds greater than 130 kt south of 7 N. It was also the costliest storm to affect the Philippines and killed more than 1000 residents. Minimum Arctic sea ice extent in September and Northern Hemisphere snow cover extent in June both reached new record lows. June snow cover extent is now declining at a faster rate (-17.6% per decade) than September sea ice extent (-13.0% per decade). Permafrost temperatures reached record high values in northernmost Alaska. A new melt extent record occurred on 11-12 July on the Greenland ice sheet; 97% of the ice sheet showed some form of melt, four times greater than the average melt for this time of year. The climate in Antarctica was relatively stable overall. The largest maximum sea ice extent since records begain in 1978 was observed in September 2012. In the stratosphere, warm air led to the second smallest ozone hole in the past two decades. Even so, the springtime ozone layer above Antarctica likely will not return to its early 1980s state until about 2060. Following a slight decline associated with the global 2 emissions from fossil fuel combustion and cement production reached a record 9.5 +/- 0.5 Pg C in 2011 and a new record of 9.7 +/- 0.5 Pg C is estimated for 2012. Atmospheric CO2 concentrations increased by 2.1 ppm in 2012, to 392.6 ppm. In spring 2012, 2 concentration exceeded 400 ppm at 7 of the 13 Arctic observation sites. Globally, other greenhouse gases including methane and nitrous oxide also continued to rise in concentration and the combined effect now represents a 32% increase in radiative forcing over a 1990 baseline. Concentrations of most ozone depleting substances continued to fall.
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| 5. |
- Xie, Sheng, et al.
(författare)
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Design and synthesis of theranostic antibiotic nanodrugs that display enhanced antibacterial activity and luminescence
- 2017
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 114:32, s. 8464-8469
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Tidskriftsartikel (refereegranskat)abstract
- We report the modular formulation of ciprofloxacin-based pure theranostic nanodrugs that display enhanced antibacterial activities, as well as aggregation-induced emission (AIE) enhancement that was successfully used to image bacteria. The drug derivatives, consisting of ciprofloxacin, a perfluoroaryl ring, and a phenyl ring linked by an amidine bond, were efficiently synthesized by a straightforward protocol from a perfluoroaryl azide, ciprofloxacin, and an aldehyde in acetone at room temperature. These compounds are propeller-shaped, and upon precipitation into water, readily assembled into stable nanoaggregates that transformed ciprofloxacin derivatives into AIE-active luminogens. The nanoaggregates displayed increased luminescence and were successfully used to image bacteria. In addition, these nanodrugs showed enhanced antibacterial activities, lowering the minimum inhibitory concentration (MIC) by more than one order of magnitude against both sensitive and resistant Escherichia coli. The study represents a strategy in the design and development of pure theranostic nanodrugs for combating drug-resistant bacterial infections.
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| 6. |
- Bu, Junling, et al.
(författare)
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Catalytic promiscuity of O-methyltransferases from Corydalis yanhusuo leading to the structural diversity of benzylisoquinoline alkaloids
- 2022
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Ingår i: Horticulture Research. - : Oxford University Press (OUP). - 2662-6810 .- 2052-7276. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- O-methyltransferases play essential roles in producing structural diversity and improving the biological properties of benzylisoquinoline alkaloids (BIAs) in plants. In this study, Corydalis yanhusuo, a plant used in traditional Chinese medicine due to the analgesic effects of its BIA-active compounds, was employed to analyze the catalytic characteristics of O-methyltransferases in the formation of BIA diversity. Seven genes encoding O-methyltransferases were cloned, and functionally characterized using seven potential BIA substrates. Specifically, an O-methyltransferase (CyOMT2) with highly efficient catalytic activity of both 4′- and 6-O-methylations of 1-BIAs was found. CyOMT6 was found to perform two sequential methylations at both 9- and 2-positions of the essential intermediate of tetrahydroprotoberberines, (S)-scoulerine. Two O-methyltransferases (CyOMT5 and CyOMT7) with wide substrate promiscuity were found, with the 2-position of tetrahydroprotoberberines as the preferential catalytic site for CyOMT5 (named scoulerine 2-O-methyltransferase) and the 6-position of 1-BIAs as the preferential site for CyOMT7. In addition, results of integrated phylogenetic molecular docking analysis and site-directed mutation suggested that residues at sites 172, 306, 313, and 314 in CyOMT5 are important for enzyme promiscuity related to O-methylations at the 6- and 7-positions of isoquinoline. Cys at site 253 in CyOMT2 was proved to promote the methylation activity of the 6-position and to expand substrate scopes. This work provides insight into O-methyltransferases in producing BIA diversity in C. yanhusuo and genetic elements for producing BIAs by metabolic engineering and synthetic biology.
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| 7. |
- Zhao, Qiao, et al.
(författare)
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On the formation of a side product with hexahydroaporphine-like structure in the Grewe cyclization of dextromethorphan
- 2017
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Ingår i: Research on Chemical Intermediates. - : Springer Science and Business Media LLC. - 0922-6168 .- 1568-5675. ; 43:3
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Tidskriftsartikel (refereegranskat)abstract
- Factors leading to the formation of a hexahydroaporphine-like cyclizing side product were studied systematically for the first time and the ratio of this side product was controlled effectively. To understand better the electronic effect of substrates on the formation of side products, different 1-benzyloctahydroisoquinolines with substituted groups on nitrogen or benzene ring were compared. A plausible mechanism of cyclizing reaction was proposed, and key intermediates as well as transition states were analyzed using DFT calculations.
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| 8. |
- Wang, Sheng-Jun, 1981-, et al.
(författare)
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Influence of synaptic interaction on firing synchronization and spike death in excitatory neuronal networks
- 2008
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Ingår i: Physical Review E. Statistical, Nonlinear, and Soft Matter Physics. - New York : American Physical Society through the American Institute of Physics. - 1063-651X .- 1095-3787. ; 78:6, s. 061906-
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Tidskriftsartikel (refereegranskat)abstract
- We investigate the influence of efficacy of synaptic interaction on firing synchronization in excitatoryneuronal networks. We find spike death phenomena: namely, the state of neurons transits from the limit cycleto a fixed point or transient state. The phenomena occur under the perturbation of an excitatory synapticinteraction, which has a high efficacy. We show that the decrease of synaptic current results in spike deaththrough depressing the feedback of the sodium ionic current. In the networks with the spike death property thedegree of synchronization is lower and insensitive to the heterogeneity of neurons. The mechanism of theinfluence is that the transition of the neuron state disrupts the adjustment of the rhythm of the neuronsoscillation and prevents a further increase of the firing synchronization.
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| 9. |
- Chen, Sheng, et al.
(författare)
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On the First Observed Wave-Induced Stress Over the Global Ocean
- 2020
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Ingår i: Journal of Geophysical Research - Oceans. - : AMER GEOPHYSICAL UNION. - 2169-9275 .- 2169-9291. ; 125:12
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Tidskriftsartikel (refereegranskat)abstract
- Despite many investigations/studies on the surface wave-induced stress, the global feature of the wave-induced stress has not been obtained previously as that requires a simultaneous observation of wave spectra and wind on a global scale. The China France Oceanography Satellite (CFOSAT) provided an opportunity for the first time to evaluate the global wave-induced stress and its contribution to the total wind stress. In this study, the global spatial distributions of wave-induced stress and its correlated index for August to November in 2019 are presented using the simultaneous ocean surface winds and wave spectra from the CFOSAT. The main results show that the wave-induced stress is fundamentally dependent on the wind and wave fields on a global scale and shows significant temporal and spatial variations. Further analyses indicate that there is an upward momentum flux under strong swells and low wind speeds (below similar to 5 m/s), and an anticorrelation between the dimensionless wave-induced stress and the proportion of swell energy to the total. Finally, the variations of the surface wave induced wind stress are clear asymmetric between northern and southern hemispheres in late summer but symmetric in late fall, which are closely associated with the seasonal changes in large-scale atmospheric circulation.
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| 10. |
- Dimopoulou, Efi, et al.
(författare)
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3 D нэгж талбарын анхны бүртгэлүүд (Initial Registration of 3D Parcelels)
- 2024
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Ingår i: 3DКАДАСТРЫН ШИЛДЭГ ТУРШЛАГУУД (Best Practices 3D Cadastres). - : International Federation of Surveyors (FIG). - 9788792853646 ; , s. 67-94
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Гурван хэмжээст (3 D үл хөдлөх хөрөнгийн талаарх ойлголт, үзэл баримтлалууд 90 ээд онысүүл үеэс эхлэн газар зохион байгуулалтын баримт бичиг болон судалгааны бүтээлүүдэдтусах болсон билээ. Өнөө цагт бид 3 D кадастрын талаар хийгдсэн судалгаа, өргөн цархүрээтэй ойлголт, шилд эг туршлагуудыг олон ном, судалгааны бүтээлүүдээс харж болно.Хэдий тийм боловч ихэнх улс орнуудад 3 D үл хөдлөх хөрөнгийн эрх зүйн зохицуулалтуудыгнарийвчлан тодорхойлж 3 D кадастрын системийг эрхлэх асуудал одоо хүртэл нарийншийдэгдэ эгүй, судлагдаагүй хэвээр байна.Энэ бүлэгт Европ, Хойд болон Латин Америк, Ойрх Дорнод, Австралийн нийт 15 өөр эрхзүйн орчин дахь 3 D үл хөдлөх хөрөнгийн үзэл баримтлалыг зохиогчдын өөрсдийнх ньүндэсний туршлагад тулгуурлан харьцуулан авч үзсэн болно. Түүнчлэн Герман, Франц,Скандинав з эрэг өөр өөр ялгаатай иргэний эрх зүйн орчин дэх 3 D кадастрын системийнжишээг авч үзсэн нь эдгээр улстай ижил төрлийн эрх зүйн систем хөгжсөн бусад орнуудад3D кадастрын системийг нэвтрүүлэхэд тус нэмэр болох буй заа. Гэхдээ эдгээр жишээ, сайнтуршлагууд нь 3 D кадастрын системийг нэвтрүүлэх, хэрэгжүүлэх гэх мэт өөр өөр үе шатандявж байгааг анхаарна биз ээ. Энэ нь 3 D үл хөдлөх хөрөнгийн үзэл баримтлал, 3 D кадастрынсистемийг нэвтрүүлэхэд тулгамдаж буй асуудлуудыг тодорхойлоход хувь нэмэр оруулахынзэрэгцээ тухайн эрх зүйн орчинд хараахан бий болоогүй байгаа бэрхшээл, асуудлуудыг
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