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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Smith, Jennifer A, et al.
(författare)
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Genome-wide association study identifies 74 loci associated with educational attainment
- 2016
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Ingår i: Nature (London). - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 533:7604, s. 539-542
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Tidskriftsartikel (refereegranskat)abstract
- Educational attainment is strongly influenced by social and other environmental factors, but genetic factors are estimated to account for at least 20% of the variation across individuals. Here we report the results of a genome-wide association study (GWAS) for educational attainment that extends our earlier discovery sample of 101,069 individuals to 293,723 individuals, and a replication study in an independent sample of 111,349 individuals from the UK Biobank. We identify 74 genome-wide significant loci associated with the number of years of schooling completed. Single-nucleotide polymorphisms associated with educational attainment are disproportionately found in genomic regions regulating gene expression in the fetal brain. Candidate genes are preferentially expressed in neural tissue, especially during the prenatal period, and enriched for biological pathways involved in neural development. Our findings demonstrate that, even for a behavioural phenotype that is mostly environmentally determined, a well-powered GWAS identifies replicable associated genetic variants that suggest biologically relevant pathways. Because educational attainment is measured in large numbers of individuals, it will continue to be useful as a proxy phenotype in efforts to characterize the genetic influences of related phenotypes, including cognition and neuropsychiatric diseases.
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| 4. |
- Zheng, Jiapeng, et al.
(författare)
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Hybrid optofluidics and three-dimensional manipulation based on hybrid photothermal waveguides
- 2018
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Ingår i: NPG Asia Materials. - : Nature Publishing Group. - 1884-4049 .- 1884-4057. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Despite enormous breakthroughs in lab-on-a-chip techniques, light-driven manipulation faces two long-standing challenges: the ability to achieve both multiform manipulation and tunable manipulation range and the means to avoid potential thermal damage to the targets. By harnessing the optical heating of hybrid photothermal waveguides (HPW), we develop a hybrid optofluidic technique involving buoyancy and thermocapillary convection to achieve fluid transport with controllable modes and tunable strength. Switching of the optofluidic mode from buoyancy to thermocapillary convection, namely, from vertical to horizontal vortices, is employed for three-dimensional manipulation. The strong confinement and torque in the vortices are capable of trapping and rotating/spinning particles at the vortex centers rather than the HPW. Buoyancy convection provides a trapping circle to achieve collective trapping and vertical rotation/spin, while thermocapillary convection offers a trapping lattice to achieve distributed trapping and horizontal rotation/spin. By integrating micro/nanoparticles with various properties and sizes, further investigations of the optofluidic arrangement, mixing, and synthesis will broaden the potential applications of the hybrid optofluidic technique in the fields of lab-on-a-chip, materials science, chemical synthesis and analysis, photonics, and nanoscience.
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| 5. |
- Berndt, Sonja I., et al.
(författare)
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Genome-wide meta-analysis identifies 11 new loci for anthropometric traits and provides insights into genetic architecture
- 2013
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:5, s. 501-U69
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Tidskriftsartikel (refereegranskat)abstract
- Approaches exploiting trait distribution extremes may be used to identify loci associated with common traits, but it is unknown whether these loci are generalizable to the broader population. In a genome-wide search for loci associated with the upper versus the lower 5th percentiles of body mass index, height and waist-to-hip ratio, as well as clinical classes of obesity, including up to 263,407 individuals of European ancestry, we identified 4 new loci (IGFBP4, H6PD, RSRC1 and PPP2R2A) influencing height detected in the distribution tails and 7 new loci (HNF4G, RPTOR, GNAT2, MRPS33P4, ADCY9, HS6ST3 and ZZZ3) for clinical classes of obesity. Further, we find a large overlap in genetic structure and the distribution of variants between traits based on extremes and the general population and little etiological heterogeneity between obesity subgroups.
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| 6. |
- Li, Zongbao, et al.
(författare)
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Upconversion Luminescence of Graphene Oxide through Hybrid Waveguide
- 2018
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Ingår i: The Journal of Physical Chemistry C. - : AMER CHEMICAL SOC. - 1932-7447 .- 1932-7455. ; 122:29, s. 16866-16871
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Tidskriftsartikel (refereegranskat)abstract
- Phonon-assisted upconversion is a promising way to generate short-wavelength emissions under excitation of long wavelength based on unique anti-Stokes luminescence properties. Graphene oxide nanosheets (GONs) exhibit excellent optical properties owing to quantum confinement and edge effects, which have driven research into fundamental principles and potential applications. Here, we experimentally demonstrate upconversion emission by exciting an easily fabricated GON hybrid waveguide (GHVV) with enhanced photothermal effects. The results reveal different origins of short-wavelength range and long-wavelength range in the upconversion spectra, whereas the emissive surface defects of GONs and GHW structure play significant roles in the behavior of photoluminescence. Introducing other upconversion materials to promote emission efficiency, the hybrid waveguide system might readily provide the possibility for the construction of upconversion fiber lasers and remote control of the upconversion luminescence.
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