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1.
  • Biswal, Ajaya K., et al. (författare)
  • Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield
  • 2014
  • Ingår i: Biotechnology for Biofuels. - : BioMed Central / Springer Verlag (Germany). - 1754-6834 .- 1754-6834. ; 7, s. 11-
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.
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3.
  • Das, D. K., et al. (författare)
  • Scratch adhesion testing of plasma-sprayed yttria-stabilized zirconia coatings
  • 1991
  • Ingår i: Surface and Coatings Technology. - 02578972 (ISSN) ; 46:3, s. 331-345
  • Tidskriftsartikel (refereegranskat)abstract
    • The suitability of scratch adhesion testing, usually used for determining the critical load for thin hard coatings like TiC and TiN, in characterizing plasma-sprayed yttria-stabilized zirconia coatings is demonstrated. The effects of loading rate and scratching speed on the critical load of these sprayed coatings were studied. Although some peculiarities in acoustic signal-load plot were observed at high values of loading rate and scratching speed, it was found that these intrinsic parameters, at low and medium values, do not have any prominent effect on the critical load. © 1991.
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