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> Svensson Maria A. 1980 >
A Comparative Study...
A Comparative Study of ERG Status Assessment on DNA, mRNA, and Protein Levels Using Unique Samples from a Swedish Biopsy Cohort
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- Svensson, Maria A., 1980- (författare)
- Region Örebro län,Department of Laboratory Medicine, Department of Urology, Örebro University Hospital, Örebro, Sweden
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- Perner, Sven (författare)
- Inst Pathol, Dept Prostate Canc Res, Univ Hosp Bonn, Bonn, Germany
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- Ohlson, Anna-Lena (författare)
- Department of Laboratory Medicine, University Hospital of Örebro, Örebro, Sweden; Department of Urology, University Hospital of Örebro, Örebro, Sweden
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- Day, John R. (författare)
- Hol Gen Probe, San Diego CA, USA
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- Groskopf, Jack (författare)
- Hol Gen Probe, San Diego CA, USA
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- Kirsten, Robert (författare)
- Inst Pathol, Dept Prostate Canc Res, Univ Hosp Bonn, Bonn, Germany
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- Sollie, Thomas (författare)
- Department of Laboratory Medicine, University Hospital of Örebro, Örebro, Sweden
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- Helenius, Gisela, 1973- (författare)
- Region Örebro län,Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden
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- Andersson, Swen-Olof, 1949- (författare)
- Region Örebro län,Department of Urology, Örebro University Hospital, Örebro, Sweden
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- Demichelis, Francesca (författare)
- Ctr Integrat Biol, Univ Trento, Trento, Italy; Inst Computat Biomed, Weill Cornell Med Coll, New York NY, USA
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- Andrén, Ove, 1963- (författare)
- Region Örebro län,Department of Urology, Örebro University Hospital, Örebro, Sweden
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- Rubin, Mark A. (författare)
- Dept Pathol & Lab Med, Weill Cornell Med Coll, New York NY, USA
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(creator_code:org_t)
- Lippincott Williams & Wilkins, 2014
- 2014
- Engelska.
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Ingår i: Applied immunohistochemistry & molecular morphology (Print). - : Lippincott Williams & Wilkins. - 1541-2016 .- 1533-4058. ; 22:2, s. 136-141
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The ERG rearrangement is identified in approximately 50% of prostate cancer screened cohorts and is known to be highly specific. This genetic aberration, most commonly leading to the TMPRSS2-ERG fusion, but also SLC45A3-ERG or NDRG1-ERG fusions, all leading to an overexpression of a truncated ERG protein. Most studies have applied in situ hybridization (FISH) methods or mRNA-based assays to investigate the ERG status. Recently, studies showed that ERG protein levels assessed by ERG antibodies can be used as a surrogate marker for ERG rearrangement. In the current study, we investigate ERG status on a series of diagnostic biopsies using DNA-based, mRNA-based, and protein-based assays. We formally compared 3 assay results (ie, FISH, fusion mRNA, and immunohistochemistry) to identify which method could be most appropriate to use when having limited amount of tissue. ERG rearrangement was found in 56% of the cases. Comparing ERG rearrangement status by FISH with ERG overexpression and TMPRSS2-ERG fusion transcript we found 95.1% (154/162, Fisher exact test 9.50E-36) and 85.2% (138/162, Fisher exact test 7.26E-22) concordance, respectively. We show that the ERG antibody highly correlates with the ERG rearrangement with high sensitivity and specificity. We also identified the most common TMPRSS2-ERG isoform in the majority of ERG rearranged cases. These results provide compelling evidence that the ERG antibody can be used to further investigate the role of ERG in prostate cancer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Nyckelord
- prostate cancer
- ERG
- gene fusion
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- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Svensson, Maria ...
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Perner, Sven
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Ohlson, Anna-Len ...
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Day, John R.
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Groskopf, Jack
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Kirsten, Robert
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visa fler...
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Sollie, Thomas
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Helenius, Gisela ...
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Andersson, Swen- ...
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Demichelis, Fran ...
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Andrén, Ove, 196 ...
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Rubin, Mark A.
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Örebro universitet