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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Heinzel, Tincuta, et al.
(författare)
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Attempts, Failures, Trials and Errors. Notes on an exhibition of failed prototypes and rejected projects.
- 2019
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Ingår i: The Design Journal. - : Routledge. - 1460-6925 .- 1756-3062. ; 22, s. 1941-1956
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, we present the history, the concept and the results of " Attempts, Failures, Trials and Errors" exhibition project which was first presented in the frame of Piksel Festival in Bergen, Norway (November 2017) and later on at " Salonul de Proiecte", Bucharest, Romania (February 2018). The project aimed to incite the e-textiles artists and designers to reflect upon the way they are engaging with their failures, as well as to the way in which they use these failures to better understand the context in which they are working and to continue to experiment. Our approach reverses the common R& D constructivist methods, by using deconstruction as a process of investigation in the field of wearable technologies and e-textiles. By questioning the ideas and the concepts of failure and success, the project puts an emphasis on art's capacity to be critical, while at the same time to poetically and self-ironically address contemporary challenges and concerns.
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| 4. |
- Wiesner, Rebecca, et al.
(författare)
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An interlaboratory capillary zone electrophoresis-UV study of various monoclonal antibodies, instruments, and ε-aminocaproic acid lots
- 2023
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 44:15-16, s. 1247-1257
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Tidskriftsartikel (refereegranskat)abstract
- Capillary zone electrophoresis ultraviolet (CZE-UV) has become increasingly popular for the charge heterogeneity determination of mAbs and vaccines. The ε-aminocaproic acid (eACA) CZE-UV method has been used as a rapid platform method. However, in the last years, several issues have been observed, for example, loss in electrophoretic resolution or baseline drifts. Evaluating the role of eACA on the reported issues, various laboratories were requested to provide their routinely used eACA CZE-UV methods, and background electrolyte compositions. Although every laboratory claimed to use the He et al. eACA CZE-UV method, most methods actually deviate from He's. Subsequently, a detailed interlaboratory study was designed wherein two commercially available mAbs (Waters’ Mass Check Standard mAb [pI 7] and NISTmAb [pI 9]) were provided to each laboratory, along with two detailed eACA CZE-UV protocols for a short-end, high-speed, and a long-end, high-resolution method. Ten laboratories participated each using their own instruments, and commodities, showing excellence method performance (relative standard deviations [RSDs] of percent time-corrected main peak areas from 0.2% to 1.9%, and RSDs of migration times from 0.7% to 1.8% [n = 50 per laboratory], analysis times in some cases as short as 2.5 min). This study clarified that eACA is not the main reason for the abovementioned variations.
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