| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Schoch, CL, et al.
(författare)
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Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
- 2012
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 109:16, s. 6241-6246
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Tidskriftsartikel (refereegranskat)abstract
- Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups.
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| 3. |
- Cameron, D., et al.
(författare)
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Replica Management in the European Data Grid Project
- 2004
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Ingår i: Journal of Grid Computing. - 1570-7873 .- 1572-9184. ; 2:4, s. 341-351
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Tidskriftsartikel (refereegranskat)abstract
- Within the European DataGrid project, Work Package 2 has designed and implemented a set of integrated replica management services for use by data intensive scientific applications. These services, based on the web services model, enable movement and replication of data at high speed from one geographical site to another, management of distributed replicated data, optimization of access to data, and the provision of a metadata management tool. In this paper we describe the architecture and implementation of these services and evaluate their performance under demanding Grid conditions.
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| 4. |
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| 5. |
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| 6. |
- Kunszt, P., et al.
(författare)
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File-Based Replica Management
- 2005
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Ingår i: Future generations computer systems. - : Elsevier BV. - 0167-739X .- 1872-7115. ; 21:1, s. 115-123
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Tidskriftsartikel (refereegranskat)abstract
- Data replication is one of the best known strategies to achieve high levels of availability and fault tolerance, as well as minimal access times for large, distributed user communities using a world-wide Data Grid. In certain scientific application domains, the data volume can reach the order of several petabytes; in these domains, data replication and access optimization play an important role in the manageability and usability of the Grid.
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| 7. |
- Laure, Erwin, et al.
(författare)
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Performance Engineering in Data Grids
- 2005
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Ingår i: Concurrency and Computation. - : Wiley. - 1532-0626 .- 1532-0634. ; 17:2-4, s. 171-191
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Tidskriftsartikel (refereegranskat)abstract
- The vision of Grid computing is to facilitate worldwide resource sharing among distributed collaborations. With the help of numerous national and international Grid projects, this vision is becoming reality and Grid systems are attracting an ever increasing user base. However, Grids are still quite complex software systems whose efficient use is a difficult and error-prone task. In this paper we present performance engineering techniques that aim to facilitate an efficient use of Grid systems, in particular systems that deal with the management of large-scale data sets in the tera- and petabyte range (also referred to as data Grids). These techniques are applicable at different layers of a Grid architecture and we discuss the tools required at each of these layers to implement them. Having discussed important performance engineering techniques, we investigate how major Grid projects deal with performance issues particularly related to data Grids and how they implement the techniques presented.
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| 8. |
- Moortgat-Pick, G., et al.
(författare)
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Physics at the e(+) e(-) linear collider
- 2015
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Ingår i: European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6044 .- 1434-6052. ; 75:8
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Forskningsöversikt (refereegranskat)abstract
- A comprehensive review of physics at an e(+) e(-) linear collider in the energy range of root s = 92 GeV-3 TeV is presented in view of recent and expected LHC results, experiments from low-energy as well as astroparticle physics. The report focusses in particular on Higgs-boson, top-quark and electroweak precision physics, but also discusses several models of beyond the standard model physics such as super-symmetry, little Higgs models and extra gauge bosons. The connection to cosmology has been analysed as well.
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| 10. |
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