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Sökning: WFRF:(Tan Wen)

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  • 2019
  • Tidskriftsartikel (refereegranskat)
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  • Klionsky, Daniel J., et al. (författare)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
  • Forskningsöversikt (refereegranskat)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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  • Bravo, L, et al. (författare)
  • 2021
  • swepub:Mat__t
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  • Tabiri, S, et al. (författare)
  • 2021
  • swepub:Mat__t
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  • Chiet, Cheong Wen, et al. (författare)
  • The Integration of Lean and Green Manufacturing for Malaysian Manufacturers : A Literature Review to Explore the Synergies between Lean and Green Model
  • 2019
  • Ingår i: International Conference on Sustainable Energy and Green Technology 2018. - : Institute of Physics Publishing (IOPP).
  • Konferensbidrag (refereegranskat)abstract
    • In general, profitability and efficiency have been the main interest for organization. However, the increasing concerns for the environment from government, regulators, customers and other stakeholders has forced companies to seek for alternatives to achieve green objectives. The difficulties faced by organizations are lack of awareness and guideline in implementing green practices in their daily operation. Under constrained resources, employers are reluctant to spend money on something unclear. During the last decade, lean manufacturing seems to be visible trend in most of the manufacturing industries in Malaysia. As lean tends to emphasize on waste reduction, it provides similarity between lean and green. Therefore, it is a better atmosphere to deploy green practices and tools under existing lean manufacturing. The purpose of this paper is to present a review on the synergies between green and lean and identifying the determinants that affecting both lean and green manufacturing for Malaysian manufacturers. The determinant obtained are financial benefit, incentive, legislation, stakeholder, management commitment, technology, environmental awareness and brand image or competitiveness. Besides, the authors identified and suggested future research directions on developing an integrated lean-green model for daily operation. This study aims to assist researchers to identify the opportunities and challenges on lean-green model and this review is useful for manufacturers and government in developing manufacturing policies and guideline.
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