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Sökning: WFRF:(Tegenfeldt Jonas O.)

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  • Beech, Jason P., et al. (författare)
  • Separation of pathogenic bacteria by chain length
  • 2018
  • Ingår i: Analytica Chimica Acta. - Elsevier. - 0003-2670. ; 1000, s. 223-231
  • Tidskriftsartikel (refereegranskat)abstract
    • Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.
  • McGinn, Steven, et al. (författare)
  • New technologies for DNA analysis - a review of the READNA Project
  • 2016
  • Ingår i: New Biotechnology. - 1871-6784 .- 1876-4347. ; 33:3, s. 311-330
  • Forskningsöversikt (refereegranskat)abstract
    • <p>The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively.</p>
  • Xavier, Miguel, et al. (författare)
  • Label-free enrichment of primary human skeletal progenitor cells using deterministic lateral displacement
  • 2019
  • Ingår i: Lab on a Chip. - Royal Society of Chemistry. - 1473-0189. ; 19:3, s. 513-523
  • Tidskriftsartikel (refereegranskat)abstract
    • Skeletal stem cells (SSCs) are present in bone marrow (BM) and offer great potential for bone regenerative therapies. However, in the absence of a unique marker, current sorting approaches remain challenging in the quest for simple strategies to deliver SSCs with consistent regeneration and differentiation capacities. Microfluidics offers the possibility to sort cells marker-free, based on intrinsic biophysical properties. Recent studies indicate that SSCs are stiffer than leukocytes and are contained within the larger cell fraction in BM. This paper describes the use of deterministic lateral displacement (DLD) to sort SSCs based on cell size and stiffness. DLD is a technology that uses arrays of micropillars to sort cells based on their diameter. Cell deformation within the device can change the cell size and affect sorting - here evidenced using human cell lines and by fractionation of expanded SSCs. Following sorting, SSCs remained viable and retained their capacity to form clonogenic cultures (CFU-F), indicative of stem cell potential. Additionally, larger BM cells showed enhanced capacity to form CFU-F. These findings support the theory that SSCs are more abundant within the larger BM cell fraction and that DLD, or other size-based approaches, could be used to provide enriched SSC populations with significant implications for stem cell research and translation to the clinic.
  • Alizadehheidari, Mohammadreza, et al. (författare)
  • Nanoconfined Circular and Linear DNA : Equilibrium Conformations and Unfolding Kinetics
  • 2015
  • Ingår i: Macromolecules. - 0024-9297 .- 1520-5835. ; 48:3, s. 871-878
  • Tidskriftsartikel (refereegranskat)abstract
    • <p>Studies of circular DNA confined to nanofluidic channels are relevant both from a fundamental polymer-physics perspective and due to the importance of circular DNA molecules in vivo. We here observe the unfolding of confined DNA from the circular to linear configuration as a light-induced double-strand break occurs, characterize the dynamics, and compare the equilibrium conformational statistics of linear and circular configurations. This is important because it allows us to determine to what extent existing statistical theories describe the extension of confined circular DNA. We find that the ratio of the extensions of confined linear and circular DNA configurations increases as the buffer concentration decreases. The experimental results fall between theoretical predictions for the extended de Gennes regime at weaker confinement and the Odijk regime at stronger confinement. We show that it is possible to directly distinguish between circular and linear DNA molecules by measuring the emission intensity from the DNA. Finally, we determine the rate of unfolding and show that this rate is larger for more confined DNA, possibly reflecting the corresponding larger difference in entropy between the circular and linear configurations.</p>
  • Alizadehheidari, Mohammadreza, 1987-, et al. (författare)
  • Unfolding of nanoconfined circular DNA
  • 2015
  • Ingår i: Biophysical Journal. - 0006-3495 .- 1542-0086. ; 108:2 Supplement 1, s. 231A-231A
  • Tidskriftsartikel (övrigt vetenskapligt)
  • Barrett, Michael P., et al. (författare)
  • Microfluidics-based approaches to the isolation of African trypanosomes
  • 2017
  • Ingår i: Pathogens. - MDPI AG. - 2076-0817. ; 6:4
  • Forskningsöversikt (refereegranskat)abstract
    • African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.
  • Beech, Jason P., et al. (författare)
  • Active Posts in Deterministic Lateral Displacement Devices
  • 2019
  • Ingår i: Advanced Materials Technologies. - Wiley Online Library. - 2365-709X. ; 4:9
  • Tidskriftsartikel (refereegranskat)abstract
    • Using electrically connected metal-coated posts in a deterministic lateral displacement (DLD) device and applying electric fields, electrokinetics is used to tune separations, significantly decrease the critical size for separation, and increase the dynamic range with switching times on the order of seconds. The strength of DLD stems from its binary behavior. To first approximation, particles move in one out of two trajectories based on their effective size. For particles that are close to the threshold size, a small external force is sufficient to nudge the particles from one trajectory to another. The devices consist of arrays of cylindrical metal-coated SU-8 posts connected by an underlying metal layer. This allows the application of voltages at the post surfaces and the generation of electric field gradients between neighboring posts, causing polarizable particles to experience a dielectrophoretic (DEP) force. This force, which depends on the volume and polarizability of the particle, can be made sufficient to push particles from one trajectory into another. In this way, the critical size in a device, normally fixed by the geometry, can be tuned. What's more, adding DEP in this way allows for the simultaneous creation of multiple size fractions.
  • Beech, Jason P., et al. (författare)
  • Capillary driven separation on patterned surfaces
  • 2009
  • Ingår i: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009,Jeju, Korea, Republic of,2009-11-01 - 2009-11-05. - Chemical and Biological Microsystems Society.
  • Konferensbidrag (refereegranskat)abstract
    • Deterministic lateral displacement (DLD) is a powerful bimodal separation scheme [1] based on fluid flow through regular obstacle arrays that in its basic embodiment sends suspended particles in two different directions as a function of size. We show that without the need to seal devices and without the need for fluidic connections or pumps, particle separation can be achieved by the passive flow of a sample over a patterned surface. Risk of clogging is minimized by the movement of large particles above the obstacle array. Suitable application areas include blood fractionation and analysis of drinking water. 0
  • Beech, Jason P., et al. (författare)
  • Cell morphology and deformability in deterministic lateral displacement devices
  • 2011
  • Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011,Seattle, WA, United States,2011-10-02 - 2011-10-06.
  • Konferensbidrag (refereegranskat)abstract
    • Deterministic Lateral Displacement (DLD) devices have been used to separate particles based on size [1] and shape [2]. Here we show how DLD devices can also be used to separate particles based on their ability to deform under shear forces. Varying experimental conditions allows us to vary the relative contributions of size, morphology and deformability. The ability to distinguish between cells based on deformability with high resolution and throughput, in cheap and simple devices, could find highly interesting and relevant applications, for example in the detection of circulating tumor cells or malaria-infected blood cells.
  • Beech, Jason P., et al. (författare)
  • Gravitationally driven deterministic lateral displacement devices
  • 2009
  • Ingår i: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009,Jeju, Korea, Republic of,2009-11-01 - 2009-11-05. - Chemical and Biological Microsystems Society.
  • Konferensbidrag (refereegranskat)abstract
    • Deterministic lateral displacement (DLD) is a powerful bimodal separation scheme [1] based on regular obstacle arrays that in its basic embodiment sends particles in two different directions as a function of size. We add functionality to the technique by including gravitational forces, as a perturbation to particles transported by fluid flow, and as a way of transporting the particles through a stationary fluid.
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