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- Lettner, Thomas
(författare)
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Bright and strain-tunable semiconductor quantum dot devices
- 2021
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Optically active semiconductor quantum dots have proven to be excellent single- and entangled-photon sources, with applications in quantum optics and quantum photonics. These sources are considered crucial in the development of future photonic quantum technology, such as quantum communication, quantum computation and quantum metrology. In future quantum networks, they allow to share quantum information through optical fiber links and implement secure communication protocols based on quantum key distribution.However, there are several challenges when developing quantum dot devices in order to unlock the full potential of these quantum emitters. The ideal quantum dot source efficiently generates triggered single- and entangled-photons on-demand. It provides further high collection-efficiency, low multi-photon probability, near-unity indistinguishability and high entanglement fidelity. Finally, it also offers compatibility with other systems by providing photons with the desired spectral properties and enabling efficient photon coupling.In this thesis the development and fabrication of bright and strain-tunable quantum dot devices for single- and entangled-photon generation has been studied. It covers highly-symmetric GaAs quantum dots emitting in the near-infrared, InAs quantum dots generating photons in the telecom C-band and InAsP quantum dots embedded in InP nanowires enabling deterministic integration into photonic circuits. The main aspects of operating these quantum dots in cryogenic micro-photoluminescence experiments are described, with focus on enhancing the collection efficiency using solid immersion lenses. For strain-tunability, the focus lies on the fabrication of piezoelectric actuators as substrates for the integration of quantum dot samples by polymer-based bonding. Finally, this thesis describes the simulation, fabrication and measurement of a novel device featuring quantum dots embedded in broad-band parabolic mirror microcavities for enhanced light collection.Experimental results obtained with a variety of quantum dot devices are included: GaAs quantum dot devices featuring solid immersion lenses demonstrate record-low multi-photon probability and near-unity photon indistinguishability. Piezoelectric strain-tunable devices with InAs quantum dots emitting in the telecom C-band allow for on-demand generation of single- and entangled-photons with tunable quantum dot emission properties and high entanglement fidelity. Piezoelectric strain-tuning actuators enable further the realization of reconfigurable quantum photonic circuits featuring waveguide-integrated InAsP/InP nanowire quantum dots with tunable emission wavelength. Finally, GaAs quantum dots in microcavities with parabolic mirror integrated on piezoelectric actuators achieve an increase in brightness by one order of magnitude over planar structures while allowing to tune the emission wavelength to the atomic transition 87Rb D1 relevant for quantum memory applications.
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| 2. |
- Matthiesen, Isabelle
(författare)
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Recreating the microenvironment of the neurovascular unit
- 2022
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The neurovascular unit (NVU) comprises the blood-brain-barrier (BBB) and its surrounding astrocytes, pericytes and neurons that are embedded in the extracellular matrix (ECM). As the main function of the BBB is to protect the brain from inlet of pathogens and toxins, the specialized endothelial cells that keep the barrier tight will also hinder the passage of pharmaceuticals. Understanding the detailed microenvironment and cellular interactions involved in the development of the neurovascular unit is, therefore, an important step towards designing CNS-targeting pharmaceuticals that can pass into the brain. At the same time, the initial steps of pharmaceutical development often involve the use of animal based in vitro models with poor human translation; thus, there is a great need for novel methods to better mimic the complexity of the human NVU. Apart from conventional cell culture models, the use of micro-engineered devices, microphysiological systems (MPS), have gained popularity. The use of MPS allows for fabrication of tissue-like structures using stem cells and provide more in vivo-like parameters in terms of physical cues and dynamic flow. Various materials have been explored for chip fabrication, and biological and synthetic ECM-mimicking hydrogels have been developed for cell encapsulation. Unfortunately, models developed to date often lack either: i) relevant and reproducible cell sources, ii) materials that allow for easy chip fabrication where sensors can be integrated to understand metabolic effects and barrier integrity, or iii) animal-free defined ECM-mimicking scaffolds that support the culture of sensitive cells. This thesis presents an isogenic model of the BBB using iPSC-derived endothelial cells and astrocytes cultured in a MPS made from the non-absorbing polymer OSTE+ that allows for easy fabrication and integration of interdigitated gold electrodes for continuous barrier integrity monitoring. The model presents barrier-protective effects of the BBB-penetrating drug NACA. To better understand the metabolic attributes of astrocytes, a flow-cell sensor is evaluated for the measurement of glucose and lactate turnover during a ketogenic diet. The results imply that such a sensor is valuable for the measurement of metabolic changes and can, in the future, be integrated into MPSs.Furthermore, a model of early neuronal development is realized by using defined copper-free click chemistry to conjugate laminin to a hyaluronic-based hydrogel system for the differentiation of neuroepithelial stem cells. The use of the hydrogel is validated for bioprinting, and the first-ever printed neuroepithelial stem cells are presented. In another study astrocyte 3D culture and bioprinting is evaluated in peptide conjugated hyaluronic-based hydrogels. Unique attachment and spreading of human fetal astrocytes is observed while the common glioblastoma U87 cells display a rounded up morphology. The results of the hydrogel study imply that the defined chemistry of the hydrogel is suitable for both neuroepithelial stem cells, U87 and fetal primary astrocytes, and can in the future be integrated into MPS to circumvent the use of animal derived matrices. In summary, these results provide solutions to some of the problems to date and lay the ground work for the continuation of the development of human-relevant MPS of the NVU.
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