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- Schramm, R, et al.
(författare)
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Leukocyte adhesion in aorta and femoral artery in vivo is mediated by LFA-1
- 2004
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Ingår i: Inflammation Research. - : Springer Science and Business Media LLC. - 1420-908X .- 1023-3830. ; 53:10, s. 523-527
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs. PBS-treated controls, n = 5-6). Notably, the number of firmly adherent leukocytes in aorta and femoral artery of cytokine-stimulated LFA-1-deficient animals was reduced by 54% and 92% indicating an important role of LFA-1 in leukocyte adhesion to arterial endothelium (P < 0.05 vs. controls, n = 5-6). In addition, pretreatment of WT mice with a monoclonal antibody (mAb) directed against murine LFA-1 attenuated the leukocyte adhesive response by 60% and 86% in aorta and femoral artery, respectively (P < 0.05 vs. control mAb-treated WT, n = 5-12). Conclusion: These novel data demonstrate that cytokine-induced firm leukocyte adhesion in the mouse aorta and femoral artery is LFA-1-dependent in vivo, which may implicate an important role for this β(2)-integrin leukocyte extravasation. in arterial injury and atherogenesis.
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