Sökning: WFRF:(Tingstrom A)
> Chemin K >
Natural killer cell...
Natural killer cells and type II interferon in Ro/SSA and La/SSB autoantibody-exposed newborns at risk of congenital heart block
-
- Ivanchenko, M (författare)
- Karolinska Institutet
-
- Thorlacius, GE (författare)
- Karolinska Institutet
-
- Hedlund, M (författare)
- Karolinska Institutet
-
visa fler...
-
- Ottosson, V (författare)
- Karolinska Institutet
-
- Meneghel, L (författare)
- Karolinska Institutet
-
Bjorkander, S (författare)
-
Ossoinak, A (författare)
-
- Tingstrom, J (författare)
- Karolinska Institutet
-
- Bremme, K (författare)
- Karolinska Institutet
-
Sverremark-Ekstrom, E (författare)
-
- Gemzell-Danielsson, K (författare)
- Karolinska Institutet
-
- Sonesson, SE (författare)
- Karolinska Institutet
-
- Chemin, K (författare)
- Karolinska Institutet
-
- Wahren-Herlenius, M (författare)
- Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- 2020-10-01
- 2021
- Engelska.
-
Ingår i: Annals of the rheumatic diseases. - : BMJ. - 1468-2060 .- 0003-4967. ; 80:2, s. 194-202
- Relaterad länk:
-
http://kipublication...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Congenital heart block (CHB) with immune cell infiltration develops in the fetus after exposure to maternal Ro/La autoantibodies. CHB-related serology has been extensively studied, but reports on immune-cell profiles of anti-Ro/La-exposed neonates are lacking. In the current study, we characterised circulating immune-cell populations in anti-Ro/La+mothers and newborns, and explored potential downstream effects of skewed neonatal cell populations.MethodsIn total, blood from mothers (n=43) and neonates (n=66) was sampled at birth from anti-Ro/La+ (n=36) and control (n=30) pregnancies with or without rheumatic disease and CHB. Flow cytometry, microarrays and ELISA were used for characterising cells and plasma.ResultsSimilar to non-pregnant systemic lupus erythematosus and Sjögren-patients, anti-Ro/La+mothers had altered B-cell subset frequencies, relative T-cell lymphopenia and lower natural killer (NK)-cell frequencies. Surprisingly, their anti-Ro/La exposed neonates presented higher frequencies of CD56dimCD16hiNK cells (p<0.01), but no other cell frequency differences compared with controls. Type I and II interferon (IFN) gene-signatures were revealed in neonates of anti-Ro/La+ pregnancy, and exposure of fetal cardiomyocytes to type I IFN induced upregulation of several NK-cell chemoattractants and activating ligands. Intracellular flow cytometry revealed IFNγ production by NK cells, CD8+and CD4+T cells in anti-Ro/La exposed neonates. IFNγ was also detectable in their plasma.ConclusionOur study demonstrates an increased frequency of NK cells in anti-Ro/La exposed neonates, footprints of type I and II IFN and an upregulation of ligands activating NK cells in fetal cardiac cells after type I IFN exposure. These novel observations demonstrate innate immune activation in neonates of anti-Ro/La+pregnancy, which could contribute to the risk of CHB.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Ivanchenko, M
-
Thorlacius, GE
-
Hedlund, M
-
Ottosson, V
-
Meneghel, L
-
Bjorkander, S
-
visa fler...
-
Ossoinak, A
-
Tingstrom, J
-
Bremme, K
-
Sverremark-Ekstr ...
-
Gemzell-Danielss ...
-
Sonesson, SE
-
Chemin, K
-
Wahren-Herlenius ...
-
visa färre...
- Artiklar i publikationen
-
Annals of the rh ...
- Av lärosätet
-
Karolinska Institutet