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Sökning: WFRF:(Vaida C)

  • Resultat 1-6 av 6
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  • Kanai, M, et al. (författare)
  • 2023
  • swepub:Mat__t
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  • Felde, Vivian A., et al. (författare)
  • Compositional turnover and variation in Eemian pollen sequences in Europe
  • 2020
  • Ingår i: Vegetation History and Archaeobotany. - : Springer Science and Business Media LLC. - 0939-6314 .- 1617-6278. ; 29:1, s. 101-109
  • Tidskriftsartikel (refereegranskat)abstract
    • The Eemian interglacial represents a natural experiment on how past vegetation with negligible human impact responded to amplified temperature changes compared to the Holocene. Here, we assemble 47 carefully selected Eemian pollen sequences from Europe to explore geographical patterns of (1) total compositional turnover and total variation for each sequence and (2) stratigraphical turnover between samples within each sequence using detrended canonical correspondence analysis, multivariate regression trees, and principal curves. Our synthesis shows that turnover and variation are highest in central Europe (47-55 degrees N), low in southern Europe (south of 45 degrees N), and lowest in the north (above 60 degrees N). These results provide a basis for developing hypotheses about causes of vegetation change during the Eemian and their possible drivers.
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  • Leppert, Axel, et al. (författare)
  • Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
  • 2024
  • Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 146:28, s. 19555-19565
  • Tidskriftsartikel (refereegranskat)abstract
    • Gelation of protein condensates formed by liquid-liquid phase separation occurs in a wide range of biological contexts, from the assembly of biomaterials to the formation of fibrillar aggregates, and is therefore of interest for biomedical applications. Soluble-to-gel (sol-gel) transitions are controlled through macroscopic processes such as changes in temperature or buffer composition, resulting in bulk conversion of liquid droplets into microgels within minutes to hours. Using microscopy and mass spectrometry, we show that condensates of an engineered mini-spidroin (NT2repCTYF) undergo a spontaneous sol-gel transition resulting in the loss of exchange of proteins between the soluble and the condensed phase. This feature enables us to specifically trap a silk-domain-tagged target protein in the spidroin microgels. Surprisingly, laser pulses trigger near-instant gelation. By loading the condensates with fluorescent dyes or drugs, we can control the wavelength at which gelation is triggered. Fluorescence microscopy reveals that laser-induced gelation significantly further increases the partitioning of the fluorescent molecules into the condensates. In summary, our findings demonstrate direct control of phase transitions in individual condensates, opening new avenues for functional and structural characterization.
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  • Resultat 1-6 av 6

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