| 1. |
- Michel, M., et al.
(författare)
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Small-molecule activation of OGG1 increases oxidative DNA damage repair by gaining a new function
- 2022
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Ingår i: Science. - Stockholm : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 376:6600, s. 1471-1476
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Tidskriftsartikel (refereegranskat)abstract
- Oxidative DNA damage is recognized by 8-oxoguanine (8-oxoG) DNA glycosylase 1 (OGG1), which excises 8-oxoG, leaving a substrate for apurinic endonuclease 1 (APE1) and initiating repair. Here, we describe a small molecule (TH10785) that interacts with the phenylalanine-319 and glycine-42 amino acids of OGG1, increases the enzyme activity 10-fold, and generates a previously undescribed b,d-lyase enzymatic function. TH10785 controls the catalytic activity mediated by a nitrogen base within its molecular structure. In cells, TH10785 increases OGG1 recruitment to and repair of oxidative DNA damage. This alters the repair process, which no longer requires APE1 but instead is dependent on polynucleotide kinase phosphatase (PNKP1) activity. The increased repair of oxidative DNA lesions with a small molecule may have therapeutic applications in various diseases and aging. © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
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| 2. |
- Jiménez-Ruano, A., et al.
(författare)
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Assessing human-caused wildfire ignition likelihood across Europe
- 2023
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Ingår i: Int. Conf. Smart Sustain. Technol., SpliTech. - : Institute of Electrical and Electronics Engineers Inc.. - 9789532901283
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Konferensbidrag (refereegranskat)abstract
- Fire ignition probability is an essential component of most fire risk assessment frameworks. This study, framed within the H2020 project FirEUrisk, features a cohesive modelling approach in a set of representative regions (pilot sites; PS) in terms of fire activity across the European territory. These PS encompass different wildfire regimes in contrasting environmental settings: PS-1 Northern Europe, Kalmar Iän (South-East Sweden); PS-2 Central Europe, Southern Brandenburg and Eastern Saxony (Germany), North Bohemia (Czechia), and Lower Silesia (Poland); PS-3 Central Portugal; PS-4 Barcelona province (Spain); and PS-5 Attica region (Greece). Our main goal was to develop a common approach to model human-caused ignitions at a fine-grained spatial resolution (100 meters). For each pilot site we: (i) ascertain which factors influence ignition, hence, addressing potential differences in driving forces and, (ii) provide a spatial-explicit depiction of the patterns of ignition probability. For that propose, we fitted a Random Forest (RF) model in each PS from historical fire records (compiled by local fire agencies) and geospatial layers for land cover, accessibility, and population related factors. All models attained a high predictive accuracy, with AUCs that ranging from 0.69 (Northern Europe) to 0.89 (Attica Region). In turn, the most relevant explanatory variable was the population density that ranked most influential in four out of the five PS, followed by the fuel type, distance to roads, distance to the WUI, and percent cover of forest and wildlands. These findings are a valuable product to upscale future solutions at regional level (beyond NUTS3-type areas), conduct fire behavior modelling simulations, and enrich the science-based decisions which come from the forest and fire management agents at national and European level. © 2023 University of Split, FESB.
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| 3. |
- Alberts, D., et al.
(författare)
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In-depth profile analysis of filled alumina and titania nanostructured templates by radiofrequency glow discharge coupled to optical emission spectrometry
- 2010
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 396:8, s. 2833-2840
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Tidskriftsartikel (refereegranskat)abstract
- The development of highly ordered and self-assembled magnetic nanostructures such as arrays of Fe or Ni nanowires and their alloys is arousing increasing interest due to the peculiar magnetic properties of such materials at the nanoscale. These nanostructures can be fabricated using nanoporous anodic alumina membranes or self-assembled nanotubular titanium dioxide as templates. The chemical characterization of the nanostructured layers is of great importance to assist the optimization of the filling procedure or to determine their manufacturing quality. Radiofrequency glow discharge (RF-GD) coupled to optical emission spectrometry (OES) is a powerful tool for the direct analysis of either conducting or insulating materials and to carry out depth profile analysis of thin layers by multi-matrix calibration procedures. Thus, the capability of RF-GD-OES is investigated here for the in-depth quantitative analysis of self-aligned titania nanotubes and self-ordered nanoporous alumina filled with arrays of metallic and magnetic nanowires obtained using the template-assisted filling method. The samples analysed in this work consisted of arrays of Ni nanowires with different lengths (from 1.2 up to 5 μm) and multilayer nanowires of alternating layers with different thicknesses (of 1-2 μm) of Ni and Au, or Au and FeNi alloy, deposited inside the alumina and titania membranes. Results, compared with other techniques such as scanning electron microscopy and energy-dispersive X-ray spectroscopy, show that the RF-GD-OES surface analysis technique proves to be adequate and promising for this challenging application. © 2009 Springer-Verlag.
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