Blood plasma coagulation studied by surface plasmon resonance

• A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real-time as a function of thromboplastin and heparin concentrations. The physical reason for the SPR signal observed is discussed and 3 different models are proposed. The response curves were analyzed by multivariable curve fitting followed by feature extraction. Interesting parameters of the sigmoid curves were lag time, slope and maximum response. When thromboplastin concentrations were increased, the lag-time decreased and the slope of the curve increased. A prolonged clotting time was mostly followed by increased maximum response, with exception for samples with no or very little thromboplastin added. High heparin concentrations changed the clotting kinetics, as seen from the lag-time vs. slope relation. Atomic force microscopy (AFM) pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and fiber thickness increased with lower thromboplastin concentration. The results correlate well with present common methods.

Nyckelord

surface plasmon resonance (SPR); blood plasma coagulation; atomic force microscopy (AFM)

TECHNOLOGY

TEKNIKVETENSKAP

Publikations- och innehållstyp

ref (ämneskategori)

kon (ämneskategori)