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1.
  • Klionsky, Daniel J., et al. (författare)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • Ingår i: Autophagy. - : Landes Bioscience. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
  • Forskningsöversikt (refereegranskat)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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2.
  • Ablikim, M., et al. (författare)
  • Amplitude analysis of the KSKS system produced in radiative J /psi decays
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
  • Tidskriftsartikel (refereegranskat)abstract
    • An amplitude analysis of the KSKS system produced in radiative J/psi decays is performed using the (1310.6 +/- 7.0) x 10(6) nip decays collected by the BESIII detector. Two approaches are presented. A mass-dependent analysis is performed by parametrizing the KSKS invariant mass spectrum as a sum of Breit-aligner line shapes. Additionally, a mass-independent analysis is performed to extract a piecewise function that describes the dynamics of the KSKS system while making minimal assumptions about the properties and number of poles in the amplitude. The dominant amplitudes in the mass-dependent analysis include the f(0)(1710), f(0)(2200), and f(2)'(1525). The mass-independent results, which are made available as input for further studies, are consistent with those of the mass-dependent analysis and are useful for a systematic study of hadronic interactions. The branching fraction of radiative J/psi decays to KSKS is measured to be (8.1 +/- 0.4) x 10(-4), where the uncertainty is systematic and the statistical uncertainty is negligible.
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3.
  • Ablikim, M., et al. (författare)
  • Branching fraction measurement of J/ψ→KSKL and search for J/ψ→KSKS
  • 2017
  • Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:11
  • Tidskriftsartikel (refereegranskat)abstract
    • Using a sample of 1.31 x 10(9) J/Psi events collected with the BESIII detector at the BEPCII collider, we study the decays of J/Psi -> KSKL and KSKS. The branching fraction of J/Psi -> KSKL is determined to be B(J/Psi -> KSKL) = (1.93 +/- 0.01 (stat) +/- 0.05 (syst)) x 10(-4), which significantly improves on previous measurements. No clear signal is observed for the J/Psi -> KSKS process, and the upper limit at the 95% confidence level for its branching fraction is determined to be B(J/Psi -> KSKS) < 1.4 x 10(-8), which improves on the previous searches by 2 orders in magnitude and reaches the order of the Einstein-Podolsky-Rosen expectation.
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4.
  • Ablikim, M., et al. (författare)
  • Measurement of e(+)e(-) -> K(K)over-barJ/psi cross sections at center-of-mass energies from 4.189 to 4.600 GeV
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
  • Tidskriftsartikel (refereegranskat)abstract
    • We investigate the process e(+)e(-) -> K (K) over barJ/psi at center-of-mass energies from 4.189 to 4.600 GeV using 4.7 fb(-1) of data collected by the BESIII detector at the BEPCII collider. The Born cross sections for the reactions e(+)e(-) -> K(+)K(-)J/psi and K(S)(0)K(S)(0)J/psi are measured as a function of center-of-mass energy. The energy dependence of the cross section for e(+)e(-) -> K(+)K(-)J/psi is shown to differ from that for pi(+)pi(-)J/psi in the region around the Y(4260). In addition, there is evidence for a structure around 4.5 GeV in the e(+)e(-) -> K(+)K(-)J/psi cross section that is not present in pi(+)pi(-)J/psi.
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5.
  • Ablikim, M., et al. (författare)
  • Measurement of the absolute branching fraction of D*(s0) (2317)(+/-) -> pi D-0(s)+/-
  • 2018
  • Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 97:5
  • Tidskriftsartikel (refereegranskat)abstract
    • The process e(+)e(-) -> D*D-+(s)*(s0) (2317)(-) + c.c. is observed for the first time with the data sample of 567 pb(-1) collected with the BESIII detector operating at the BEPCII collider at a center-of-mass energy root s = 4.6 GeV. The statistical significance of the D*(s0) (2317)(+/-) signal is 5.8 sigma and the mass is measured to be (2318.3 +/- 1.2 +/- 1.2) MeV/c(2). The absolute branching fraction B(D*(s0) (2317)(+/-) -> pi D-0(s)+/-) is measured as 1.00(-0.14)(+0.00) (stat)(-0.14)(+0.00) (syst) for the first time. The uncertainties are statistical and systematic, respectively.
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6.
  • Ablikim, M., et al. (författare)
  • Measurement of the Absolute Branching Fraction of the Inclusive Decay Lambda(+)(c) -> Lambda plus X
  • 2018
  • Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:6
  • Tidskriftsartikel (refereegranskat)abstract
    • Based on an e(+)e(-) collision data sample corresponding to an integrated luminosity of 567 pb(-1) taken at the center-of-mass energy of root s = 4.6 GeV with the BESIII detector, we measure the absolute branching fraction of the inclusive decay Lambda(+)(c) -> Lambda + X to be B(Lambda(+)(c) -> Lambda + X) = (38.2(-2.2)(+2.8) +/- 0.9)% using the double-tag method, where X refers to any possible final state particles. In addition, we search for direct CP violation in the charge asymmetry of this inclusive decay for the first time, and obtain A(CP) [B(Lambda(+)(c) -> Lambda + X) - B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)]/[B(Lambda(+)(c) -> Lambda + X) + B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)] = (2.1(-6.6)(+7.0) +/- 1.6)%, a statistically limited result with no evidence of CP violation.
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7.
  • Ablikim, M., et al. (författare)
  • Measurements of the branching fractions of the singly Cabibbo-suppressed decays D0→ωη, η(')π0 and η(')η
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:5
  • Tidskriftsartikel (refereegranskat)abstract
    • By analyzing a data sample of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector operated at the BEPCII storage rings, we measure the branching fractions B(D-0 -> omega eta) = (2.15 +/- 0.17(stat) +/- 0.15(sys)) x 10(-3), B(D-0 ->eta pi(0)) = (0.58 +/- 0.05(stat) +/- 0.05(sys)) x 10(-3), B(D-0 ->eta'pi(0)) = (0.93 +/- 0.11(stat) +/- 0.09(sys)) x 10(-3), B(D-0 -> eta eta) = (2.20 +/- 0.07(stat) +/- 0.06(sys)) x 10(-3) and B(D-0 -> eta'eta) = (0.94 degrees +/- 0.25(stat) +/- 0.11(sys)) x 10(-3). We note that B(D-0 -> omega eta) is measured for the first time and that B(D-0 -> eta eta) is measured with much improved precision.
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8.
  • Ablikim, M., et al. (författare)
  • Observation of h(1)(1380) in the J/psi -> eta ' K(K)over-bar pi decay
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
  • Tidskriftsartikel (refereegranskat)abstract
    • Using 1.31 x 10(9) J/psi events collected by the BESIII detector at the BEPCII e(+)e(-) collider, we report the first observation of the h(1)(1380) in J/psi -> eta'h(1)(1380) with a significance of more than ten standard deviations. The mass and width of the possible axial-vector strangeonium candidate h(1)(1380) are measured to be M = (1423.2 +/- 2.1 +/- 7.3) MeV/c(2) and Gamma = (90.3 +/- 9.8 +/- 17.5) MeV. The product branching fractions, assuming no interference, are determined to be B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)K-+(-)+c.c.) = (1.51 +/- 0.09 +/- 0.21) x 10(-4) in eta'K+K-pi(0) mode and B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)(K) over bar +c.c.) =(2.16 +/- 0.12 +/- 0.29) x 10(-4) in eta'(KSK +/-)-K-0 pi(-/+) mode. The first uncertainties are statistical and the second are systematic. Isospin symmetry violation is observed in the decays h(1)(1380) K*(892)K-+(-) + c.c. and h(1)(1380) -> K*(892)(0)(K) over bar (0) + c.c.. Based on the measured h(1)(1380) mass, the mixing angle between the states h(1)(1170) and h(1)(1380) is determined to be (35.9 +/- 2.6)degrees, consistent with theoretical expectations.
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9.
  • Ablikim, M., et al. (författare)
  • Study of the decays D+-> eta(('))e(+)nu(e)
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:9
  • Tidskriftsartikel (refereegranskat)abstract
    • The charm semileptonic decays D+ -> eta e(+)nu(e) and D+ -> eta'e(+)nu(e) are studied with a sample of e(+)e(-) collision data corresponding to an integrated luminosity of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector. We measure the branching fractions for D+ -> eta e(+)upsilon(e) to be (10.74 +/- 0.81 +/- 0.51)x10(-4), and for D+ -> eta'e(+)nu(e) to be (1.91 +/- 0.51 +/- 0.13) x 10(-4), where the uncertainties are statistical and systematic, respectively. In addition, we perform a measurement of the form factor in the decay D+ -> eta e(+)nu(e) . All the results are consistent with those obtained by the CLEO-c experiment.
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10.
  • Ablikim, M., et al. (författare)
  • Amplitude analysis of D0 -> K -π+π+π-
  • 2017
  • Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 95:7
  • Tidskriftsartikel (refereegranskat)abstract
    • We present an amplitude analysis of the decay D-0 -> K- pi(+)pi(+)pi(-) based on a data sample of 2.93 fb(-1) acquired by the BESIII detector at the psi(3770) resonance. With a nearly background free sample of about 16000 events, we investigate the substructure of the decay and determine the relative fractions and the phases among the different intermediate processes. Our amplitude model includes the two-body decays D-0 -> (K) over bar*(0)rho(0), D-0 -> K- a(1)(+) (1260) and D-0 -> K-1(-)(1270)pi(+), the three-body decays D-0 -> K-1(-)*(0)pi(+)pi(-) and D-0 -> K- pi(+)rho(0), as well as the four-body nonresonant decay D-0 -> K- pi(+)pi(+)pi(-). The dominant intermediate process is D-0 -> K(-)a(1)(+)(1260)accounting for a fit fraction of 54.6%.
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