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The tuatara genome reveals ancient features of amniote evolution

Gemmell, Neil J. (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Rutherford, Kim (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Prost, Stefan (author)
Senckenberg Museum, LOEWE Ctr Translat Biodivers Genom, Frankfurt, Germany; Natl Zool Garden, South African Natl Biodivers Inst, Pretoria, South Africa
show more...
Tollis, Marc (author)
Arizona State Univ, Sch Life Sci, Tempe, AZ USA; No Arizona Univ, Sch Informat Comp & Cyber Syst, Flagstaff, AZ 86011 USA
Winter, David (author)
Massey Univ, Sch Fundamental Sci, Palmerston North, New Zealand
Macey, J. Robert (author)
Peralta Genom Inst, Oakland, CA USA
Adelson, David L. (author)
Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia
Suh, Alexander (author)
Uppsala universitet,Evolutionsbiologi,Systematisk biologi
Bertozzi, Terry (author)
Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia; South Australian Museum, Evolutionary Biol Unit, Adelaide, SA, Australia
Grau, José H. (author)
Amedes Med Dienstleistungen, Amedes Genet, Berlin, Germany; Humboldt Univ, Leibniz Inst Evolut & Biodiversitatsforsch, Museum Nat Kunde Berlin, Berlin, Germany
Organ, Chris (author)
Montana State Univ, Dept Earth Sci, Bozeman, MT 59717 USA
Gardner, Paul P. (author)
Univ Otago, Dept Biochem, Dunedin, New Zealand
Muffato, Matthieu (author)
European Bioinformat Inst, European Mol Biol Lab, Hinxton, England
Patricio, Mateus (author)
European Bioinformat Inst, European Mol Biol Lab, Hinxton, England
Billis, Konstantinos (author)
European Bioinformat Inst, European Mol Biol Lab, Hinxton, England
Martin, Fergal J. (author)
European Bioinformat Inst, European Mol Biol Lab, Hinxton, England
Flicek, Paul (author)
European Bioinformat Inst, European Mol Biol Lab, Hinxton, England
Petersen, Bent (author)
Univ Copenhagen, Fac Hlth & Med Sci, GLOBE Inst, Sect Evolutionary Genom, Copenhagen, Denmark
Kang, Lin (author)
Edward Via Coll Osteopath Med, Blacksburg, VA USA
Michalak, Pawel (author)
Edward Via Coll Osteopath Med, Blacksburg, VA USA; Virginia Maryland Coll Vet Med, Ctr One Hlth Res, Blacksburg, VA USA; Univ Haifa, Inst Evolut, Haifa, Israel
Buckley, Thomas R. (author)
Manaaki Whenua Landcare Res, Auckland, New Zealand; Univ Auckland, Sch Biol Sci, Auckland, New Zealand
Wilson, Melissa (author)
Arizona State Univ, Sch Life Sci, Tempe, AZ USA
Cheng, Yuanyuan (author)
Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia
Miller, Hilary (author)
Biomatters, Auckland, New Zealand
Schott, Ryan K. (author)
Smithsonian Inst, Natl Museum Nat Hist, Dept Vertebrate Zool, Washington, DC 20560 USA
Jordan, Melissa D. (author)
New Zealand Inst Plant & Food Res, Auckland, New Zealand
Newcomb, Richard D. (author)
New Zealand Inst Plant & Food Res, Auckland, New Zealand
Arroyo, José Ignacio (author)
Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago, Chile
Valenzuela, Nicole (author)
Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA USA
Hore, Tim A. (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Renart, Jaime (author)
CSIC UAM, Inst Invest Biomed Alberto Sols, Madrid, Spain
Peona, Valentina (author)
Uppsala universitet,Evolutionsbiologi,Systematisk biologi
Peart, Claire R. (author)
Uppsala universitet,Evolutionsbiologi,Ludwig Maximilian Univ Munich, Fac Biol, Div Evolutionary Biol, Planegg Martinsried, Germany
Warmuth, Vera M. (author)
Uppsala universitet,Evolutionsbiologi,Ludwig Maximilian Univ Munich, Fac Biol, Div Evolutionary Biol, Planegg Martinsried, Germany
Zeng, Lu (author)
Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia
Kortschak, R. Daniel (author)
Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia
Raison, Joy M. (author)
Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia
Velásquez Zapata, Valeria (author)
Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA USA
Wu, Zhiqiang (author)
Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA USA
Santesmasses, Didac (author)
Univ Pompeu Fabra UPF, Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Barcelona, Spain
Mariotti, Marco (author)
Univ Pompeu Fabra UPF, Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Barcelona, Spain
Guigó, Roderic (author)
Univ Pompeu Fabra UPF, Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Barcelona, Spain
Rupp, Shawn M. (author)
Arizona State Univ, Sch Life Sci, Tempe, AZ USA
Twort, Victoria G. (author)
Manaaki Whenua Landcare Res, Auckland, New Zealand; Univ Auckland, Sch Biol Sci, Auckland, New Zealand
Dussex, Nicolas (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Taylor, Helen (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Abe, Hideaki (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Bond, Donna M. (author)
Univ Otago, Dept Anat, Dunedin, New Zealand
Paterson, James M. (author)
Univ Canterbury, Sch Biol Sci, Christchurch, New Zealand
Mulcahy, Daniel G. (author)
Smithsonian Inst, Natl Museum Nat Hist, Global Genome Initiat, Washington, DC 20560 USA
Gonzalez, Vanessa L. (author)
Smithsonian Inst, Natl Museum Nat Hist, Global Genome Initiat, Washington, DC 20560 USA
Barbieri, Charles G. (author)
Peralta Genom Inst, Oakland, CA USA
Demeo, Dustin P. (author)
Peralta Genom Inst, Oakland, CA USA
Pabinger, Stephan (author)
Austrian Inst Technol AIT, Ctr Hlth & Bioresources, Mol Diagnost, Vienna, Austria
Van Stijn, Tracey (author)
AgResearch, Invermay Agr Ctr, Mosgiel, New Zealand
Clarke, Shannon (author)
AgResearch, Invermay Agr Ctr, Mosgiel, New Zealand
Ryder, Oliver (author)
San Diego Zoo Inst Conservat Res, Escondido, CA USA
Edwards, Scott V. (author)
Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA.;Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA
Salzberg, Steven L. (author)
Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD USA
Anderson, Lindsay (author)
Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand
Nelson, Nicola (author)
Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand
Stone, Clive (author)
Ngatiwai Trust Board, Whangarei, New Zealand
Smillie, Jim (author)
Edmonds, Haydn (author)
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 (creator_code:org_t)
2020-08-05
2020
English.
In: Nature. - : Springer Nature. - 0028-0836 .- 1476-4687. ; 584:7821, s. 403-409
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The tuatara (Sphenodon punctatus)—the only living member of the reptilian order Rhynchocephalia (Sphenodontia), once widespread across Gondwana1,2—is an iconic species that is endemic to New Zealand2,3. A key link to the now-extinct stem reptiles (from which dinosaurs, modern reptiles, birds and mammals evolved), the tuatara provides key insights into the ancestral amniotes2,4. Here we analyse the genome of the tuatara, which—at approximately 5 Gb—is among the largest of the vertebrate genomes yet assembled. Our analyses of this genome, along with comparisons with other vertebrate genomes, reinforce the uniqueness of the tuatara. Phylogenetic analyses indicate that the tuatara lineage diverged from that of snakes and lizards around 250 million years ago. This lineage also shows moderate rates of molecular evolution, with instances of punctuated evolution. Our genome sequence analysis identifies expansions of proteins, non-protein-coding RNA families and repeat elements, the latter of which show an amalgam of reptilian and mammalian features. The sequencing of the tuatara genome provides a valuable resource for deep comparative analyses of tetrapods, as well as for tuatara biology and conservation. Our study also provides important insights into both the technical challenges and the cultural obligations that are associated with genome sequencing.

Subject headings

NATURVETENSKAP  -- Biologi -- Evolutionsbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Evolutionary Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Genetik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Genetics (hsv//eng)

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